Downloadable Lab Filtration Resources to Suit Your Individual Laboratory Needs
Download our free application notes and cutting-edge research.
The Sartorius Lab Filtration portfolio knowledgebase offers usable, informative, and innovative resources scientists can use today. Browse the selection below to find the resource best suited to your lab filtration needs.
How to Increase Recovery at Critical Protein Samples: Impact of Syringe Filter Membrane, Volume and pH
Protein loss during sample preparation can be an obstacle to reliable product quantitation in biological, biotechnological and biopharmaceutical settings.
Claristep Filtration Assembly: A Novel Sample Preparation Method Suitable for the Analytics of Phthalates in Solid Environmental Samples
The filtration clean-up of particle laden environmental samples for phthalates analytics is a common step in sample preparation prior to GC-MS. Here, we demonstrate that Claristep® syringeless filtration units 0.45 μm and 0.2 μm are suitable for the filtration clean-up of phthalate samples. A mix of eight phthalates and one adipate was filtered at a concentration of 10 μg/mL with neither detectable adsorption nor releasing of these compounds from the filter unit. Furthermore, it was shown by GC-MS screening that no detectable compounds were extracted.
Impact of the Claristep® Filtration System on Recovery and Adsorption of Various Therapeutic Proteins at Low Sample Volumes
We tested the novel filter device Claristep® for the preparation of protein samples containing one of four different biotherapeutic molecules. In comparison to the standard protocols for sample preparation prior to analysis by either reversed-phase or SEC HPLC, the Claristep® filter did not exhibit a significant difference in protein concentration if the target molecules were present with at least 1.0 g L-1. A significantly reduced product concentration was observed in the Claristep® filtered samples compared to the standard protocol for a monoclonal antibody, if the concentrations were lower. The protein adsorption to the filter material was well described by a Langmuir adsorption isotherm. Additionally, the Claristep® filters facilitated a rapid sample preparation with minimal volume losses.
Concentration to a defined final volume with Vivaspin® Turbo 15, Vivaspin® Turbo 4 and Vivaspin® 500
Learn how you can use Vivaspin® Turbo 15, Vivaspin® Turbo 4 and Vivaspin® 500 concentrators to concentrate to defined final volumes. By adding a particular volume to the filtrate vessel prior to the concentration, the final volume of the concentrate can be adjusted accurately.
Application Note: Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators
Vivaspin® centrifugal concentrators, with patented vertical membrane technology, combine fast filtration with high recovery of target proteins. This makes Vivaspin® the technology of choice for desalting or buffer exchange, avoiding lengthy dialysis steps.
Ergonomic and Efficient Filtration for Food Analytics
In food analytics, clarification by membrane filtration is often an important step in sample preparation for processes like HPLC. Designed for parallel processing of up to eight samples, the Claristep filtration system was examined to determine its suitability for the filtration of food samples. Fourteen commercially available food samples – among them very particle-rich dispersions – were filtered at various levels of dilution with the syringeless Claristep® 0.45 µm and 0.2 µm pore size filters and the manual Claristep® base. Suitable dilutions were also tested with an established syringe filtration system, and the two techniques were compared with regard to ease and comfort of use. The results show which levels of dilution can be expected to result in successful filtration. In comparison to conventional filtration systems, testers consistently reported better ergonomic handling and significant time savings in sample preparation with Claristep.
Laboratory Ultrafiltration: How to Choose the Optimal Device & Method
How to choose the optimal ultrafiltration device and method for proteins, viruses, DNA, polymers, nanoparticles, exosomes, and more!
Your Practical Guide To Basic Laboratory Techniques
“You need to learn to walk before you can run” is a saying many of us probably heard when we were children. The clear message here is that there are some basic skills we need to master before we can move on to the next level. And there are plenty of good reasons that following this mantra will set one up for success, not the least of which—to continue the metaphor—is avoiding tripping and falling on your face.
Treatment of Vivaspin® concentrators for improved recovery of low-concentrated protein samples
Read our application note, “Treatment of Vivaspin® concentrators for improved recovery of low-concentrated protein samples” to learn a pre-treatment process for improving recovery of low-concentration proteins samples.
Application Note: Lab Ultrafiltration – Tips and Tricks
Although performing sample concentration and buffer exchange using an ultrafiltration device is relatively simple, some tricks of the trade can improve your recovery or speed up your work flow considerably.
Laboratory Ultrafiltration FAQ
How can you reduce protein degradation during concentration? What ultrafiltration method is best suited for virus concentration? Get the answers to these questions and more in our FAQs for Laboratory Ultrafiltration guide.
How to choose the optimal housing and membrane material for your application
Labs use syringe filters for a variety of applications. These may include sterile filtration, sample preparation, and clarification of media additives, buffers, chemical reagents, drugs, and gases. Innovative design features coupled with the largest surface areas and fastest flow rates make Sartorius Minisart syringe filters the quintessential choice for all your syringe filtration needs.