Passivation of Vivaspin to Improve Protein Recoveries
Authors: Rik McRae, Claudia Naumann, Kristin Hoehne, Robert Zeidle | Last updated: 05 November 2020
Overview
When a protein is only available in low quantities, it must be handled with care to minimize loss and maximize the amount which will be available for your experiments.
While filter housing and membrane materials are selected for their low binding properties, very low levels of adsorption can still occur. As a result, any losses can become more significant and noticeable when working with nanogram to microgram quantities of protein.
This Application Note presents a simple method and suggested reagents for the treatment of Vivaspin® centrifugal concentrators prior to sample concentration. By following this process, time and cost savings can be realized by increasing the recovery of low protein quantities.
- Document type: Application Note
- Page count: 4
- Read time: 6 minutes
Key Takeaways
- Summary of non-specific protein adsorption
- Suggested reagents for passivation
- Passivation process for Vivaspin® centrifugal concentrators
- Protein recoveries from untreated vs. passivated ultrafilters
This Resource is Designed for:
- Researchers in laboratories expressing and purifying proteins for structural and functional analyses, assays, and biologics discovery/cell line/molecule development
Applications Supported:
- Protein concentration
- Passivation of ultrafilters
- Minimizing non-specific protein adsorption
- Minimizing protein loss during concentration