Application Note: Lab-Scale Harvest and Clarification of Lentiviral Vectors
Authors: Jennifer J. Labisch, Andreas Pickl, Franziska Bollmann, Karl Pflanz | Last updated: January 12, 2023
Overview
This study evaluated the use of Sartoclear Dynamics®️ Lab V50 (Sartorius) comprising the diatomaceous earth (DE) filter aid for clarification of lentiviral vectors (LV) produced by HEK293 suspension cells. The effect of the DE concentration and incubation time on filtration time, yield, and turbidity reduction was analyzed in a design of experiment (DOE) approach.
Moreover, the use of Sartoclear Dynamics®️ Lab V50 was compared against the standard laboratory method (centrifugation and subsequent filtration) to examine if DE can effectively clarify LV as this filter aid is easy to handle.
- Document type: Application Note
- Page count: 8
- Read time: 14 minutes
Key Takeaways
- Higher diatomaceous earth (DE) concentrations are beneficial for higher turbidity reduction and faster filtration times.
- Lower DE concentrations are advantageous for recovering high of lentiviral vectors (LV) titers.
- The use of Sartoclear Dynamics® Lab V50 filtration systems resulted in better turbidity reduction, greater removal of contaminants and higher membrane capacity when compared to the standard method.