App Note: Handbook for Scientists: Adeno-Associated Viruses: Lab-scale Processing and Analytics

Overview

Mastering AAV Manufacturing: From Upstream Efficiency to Downstream Purity

AAVs stand at the forefront of the gene therapy revolution, offering a proven pathway to treat diverse genetic disorders. As demand accelerates, so does the need for robust, end-to-end production strategies—from transfection to final purity—to safeguard genome integrity and maximize yield.

Explore our new eBook, delivering practical tools and advanced techniques to overcome core challenges in AAV production.

Inside, you will discover:

  • Upstream Optimization: Maximize rAAV yields with insights on plasmid ratios, transfection reagents, and culture conditions.
  • Downstream Purification: Best practices for harvesting, clarification, chromatography, and UF/DF to achieve high-quality, impurity-free vectors across serotypes.
  • Analytical Characterization & Quality Control: High-throughput methods to quantify titers, assess integrity and potency, and ensure safety.
  • Scalability & Solutions: Practical guidance for transitioning from R&D to large-scale manufacturing.

Whether at lab-scale or commercial production, this eBook outlines the critical technologies to balance yield, purity, and safety.

Download today to streamline your AAV gene therapy development and production.

  • Document type:  eBook
  • Page count: 106
  • Read time: 30 minutes

 

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Key Takeaways

  • Optimize Upstream Performance: Maximize rAAV yields through careful selection of plasmid ratios, transfection reagents, and controlled culture conditions in mammalian systems like HEK293 and SF9.
  • Streamline Downstream Purification: Achieve high-quality, impurity-free vectors using best practices in harvesting, chromatography, and UF/DF, tailored to address the unique challenges of diverse AAV serotypes.
  • Implement Robust Quality Control: Leverage high-throughput and orthogonal analytical methods to accurately quantify titers, assess genome integrity, confirm potency, and ensure safety through rigorous contaminant testing.
  • Scale Seamlessly from Lab to Commercial: Apply practical strategies to transition from process development to industrial manufacturing while maintaining balance between yield, purity, and safety.

This resource is designed for:

  • Gene therapy researcher and developers, Oncologists, Lab Researchers, CROs
  • Gene therapy developer, manufacturers, CDMOs, QC labs
  • Gene therapy, AAV R&S Academia labs

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Applications supported:

  • AAV characterization
  • Clarification, Tangential Flow Filtration (TFF)
  • AAV capture using CEX
  • Steric Exclusion chromatography
  • Empty/Full Separation
  • Sterile filtration