Concentrating mAbs by Tangential Flow Filtration with Vivaflow® Cassettes Protocol
Last updated: February 2023
Overview
This lab filtration protocol describes the use of Vivaflow® TFF cassettes for concentrating antibodies - including mAbs and IgGs,- from clarified cell culture supernatants. This economical, plug-and-play solution makes tangential flow filtration more accessible to research labs for fast and easy ultrafiltration | diafiltration of larger sample volumes.
- Document type: Protocol
- Page count: 3
- Read time: 4 minutes
Key Takeaways
- A list of materials needed for antibody sample clarification, concentration and purification
- General guidelines, including tips on speeding up the process even further
- Visual overviews and step-by-step instructions of the complete workflow
- Exemplary data showing rapid reduction in sample volume during the concentration step
This Resource is Designed for:
- Researchers in laboratories expressing and purifying antibodies (IgGs, mAbs, etc.) and other proteins for structural and functional analyses, and biologics discovery/cell line/molecule development
Applications Supported:
- Cell culture clarification
- Antibody concentration
- Antibody purification
- Tangential flow filtration
- Crossflow ultrafiltration
- Membrane chromatography
- Protein A affinity chromatography
