Faster Diafiltration and Ultrafiltration of LV with Vivaflow® SU Application Highlight
Authors: Jennifer J Labisch, John S Cashman | Last updated: March 2024
Overview
Lentiviral vectors (LV) are widely used for gene therapy and genetically modified cell therapies, with many clinical trials in the pipeline. A typical downstream processing workflow for LV comprises clarification, purification, diafiltration, ultrafiltration, and finally sterile filtration. The diafiltration and ultrafiltration steps are usually performed after purification, to formulate the LV into a suitable storage buffer and to concentrate it.
- Document type: Application Highlight
- Page count: 4
- Read time: 2 minutes
Key Takeaways
- Learn how TFF can be used for buffer exchange and concentration of lentiviral vectors
- Recognize the enhanced performance of Vivaflow® SU for diafiltration and ultrafiltration
- See how diafiltration process times can be cut by 36% compared to Vivaflow® 50
- Understand that switching from Vivaflow® 50 to Vivaflow® SU will not affect target recoveries
This Resource is Designed for:
- Process development scientists
- Research scientists
- Vaccine development scientists
Applications Supported:
- Diafiltration
- Lentiviral Vectors
- Ultrafiltration
