Lab-Scale Concentration of a Clarified AAV8 Lysate by TFF Application Note
Authors: Lena Rübeling, Caroline Trebini, John S Cashman, Klaus Schöne
Last updated: October 2024
Overview
Adeno-associated viruses (AAV) are pivotal in gene therapy due to their non-pathogenic nature and ability to transduce various cell types. The aim of this study was to identify and characterize suitable membranes for the concentration of AAV8 by tangential flow filtration (TFF) using Vivaflow® SU cassettes.
We compared two membrane types, polyethersulfone (PES) and Hydrosart® regenerated cellulose (RC), and three molecular weight cut-offs (MWCO), 30, 100 and 300 kDa. TFF performance, AAV8 recovery (genome copies and capsid titers) and total protein removal were evaluated. The 100 kDa Hydrosart® RC membrane demonstrated the highest AAV8 recovery at 95% with no detectable loss to the permeate. In addition, 29% of the total protein was removed from the retentate.
These results demonstrate the efficacy of our 100 kDa RC membrane for concentration of AAV8 and suggest its suitability for laboratory-scale production of other AAV serotypes with similar capsid sizes.
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- Document type: Application note
- Page count: 6
- Read time: 9 minutes
Key Takeaways
- Learn how AAV8 can be efficiently concentrated by tangential flow filtration (TFF)
- Discover how membrane chemistry and MWCO can influence AAV recoveries
- See how Vivaflow® SU performs in terms of process time, recovered titers and protein removal
- Find expert advice on the optimal solution to concentrate your AAV sample
This Resource is Designed for:
- Research Scientists
- AAV Development Scientists
- Viral Vector Downstream Processing Scientists
Applications Supported:
- Optimizing Crossflow Ultrafiltration
- Tangential Flow Filtration (TFF)
- Virus Concentration
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Figure 1. Cover image of Lab-Scale Concentration of a Clarified AAV8 Lysate by TFF application note.
