Application Note: Rapid Mammalian Cell Harvest without Centrifugation for Antibody Purification Using the Sartoclear Dynamics® Lab Filtration System

Authors: James Stephenson, Catherine Bladen, Ian Wilkinson | Read time: 8 min | Last Updated: May 18, 2020

The production of antibodies from mammalian cells requires separation of the cells from the antibody-containing cell culture fluid.

The most widely cited method for research scale clarification is an initial centrifugation step followed by filtration of the cleared supernatant through a 0.2, 0.22 or 0.45 μm filter, either using a hand-operated syringe or a vacuum driven bottle top filter.

But there are challenges with the conventional centrifugation and filtration process: the approaches prove to be slow, prone to clogging and low throughput and to be of low quality (e.g. endotoxin contaminated).

In this study, we describe our tests of Sartoclear Dynamics® Lab V, a novel system for rapid clarification of cell culture media without the need for centrifugation or any other costly equipment.
 

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Key Takeaways

Download our app note to learn more about how when compared to conventional methods, the Sartoclear Dynamics Lab V:

  • Enables rapid clarification of mammalian cell cultures without the need for centrifugation
  • Reduces time spent by approximately 85%
  • Maintains the same quality of the sample as conventional methods

Figure 1. Principles of clarifying cell cultures using conventional filtration 
and Sartoclear Dynamics® Lab filtration.
 

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