Endotoxin Removal by Sartobind® Lab Q and S Membrane Adsorbers - Application Guide
The presence of endotoxins in protein samples, cell culture media and reagents used for animal studies and cell culture assays can lead to significantly increased costs and delayed reporting. Therefore, a depyrogenation strategy is critical for reagents of solutions containing proteins, peptides and other macromolecules.
Overview
Document type: Application Guide
Page count: 6
Read time: 5 minutes
Last updated: December 2022
Audience: Researchers in laboratories expressing and purifying proteins which must be pyrogen-free for downstream cell-based assays and animal studies
Applications covered: Protein purification, Protein polishing, Contaminant removal, Endotoxin clearance, Depyrogenation, Pyrogen removal, Ion exchange chromatography, Membrane chromatography
In this application guide, you will learn:
- The difference between capture and flow-through polishing
- How Sartobind® Lab membrane adsorber units can be effectively removed for either method
- Summaries and data from several sample depyrogenation studies
