Endotoxin Removal by Sartobind Q | S Lab Membrane Adsorbers - Application Guide
Overview
Endotoxins (lipopolysaccharides from membranes of gram negative bacteria) make up the majority of pyrogens which must be removed or reduced from pharmaceutical products, biologics for injection, cosmetics, and cell culture media. There are many factors to consider when designing a depyrogenation strategy for media or solutions containing proteins, peptides or other macromolecules: from the type, concentration, molecular weight and isoelectric point (pI) of the target molecule of interest, to the electrolyte concentration, pH and buffer system, and interactions such as interference or aggregation. This review presents examples where Sartobind® Lab membrane adsorbers have been used for the effective removal of pyrogens from various sample types.
In this application guide, you will learn:
- The difference between capture and flow-through polishing
- How Sartobind® Lab membrane adsorber units can be effectively removed for either method
- Summaries and data from several sample depyrogenation studies
This Resource is Designed for:
- Researchers in laboratories expressing and purifying proteins which must be pyrogen-free for downstream cell-based assays and animal studies
- Pharmaceutical Industry Professionals
- Biologics Manufacturers
- Cosmetic Industry Professionals
- Laboratory Technicians and Managers
Applications Supported:
- Endotoxin Removal
- Lipopolysaccharide
- Pyrogen Removal
- Depyrogenation
- Ion Exchange Chromatography
- Membrane Adsorbers
- Membrane Chromatography
- Sartobind® Lab
- Protein Polishing
- Protein Purification
- Contaminant Removal
- Endotoxin Clearance
