Plasmid DNA Purification by Alkaline Lysis and Vivapure® IEX Membrane Adsorbers Protocol
Overview
This protocol describes a simple method to isolate and purify plasmid DNA in recombinant cloning workflows. Plasmids are first released from E. coli using a traditional alkaline lysis process. The lysate is then applied to Vivapure® IEX spin columns, where the plasmids are rapidly captured on the innovative Sartobind® Lab membrane adsorber matrix. Subsequent washing and elution steps ensure sufficient purity for downstream use, including transformation or transfection into prokaryotic or eukaryotic cells, respectively, for protein expression, or in restriction enzyme digests.
Page count: 3
Read time: 3 minutes
Last updated: April 2023
Target audience: Researchers in laboratories using protein expression and purification for structural and functional analyses
Applications covered: Cloning, Alkaline lysis, Plasmid DNA purification, Recombinant protein expression
In this protocol, you will find:
- A list of the materials required for alkaline lysis and purification
- General guidelines to consider when performing alkaline lysis and membrane chromatography
- Visual overviews and step-by-step instructions of the alkaline lysis and purification processes
- Exemplary agarose gel showing samples isolated and purified by the described method
