Establishing Small Scale AAV E/F Separation using Sartobind® Q Lab
Overview
Adeno-associated viruses (AAVs) are small, non-enveloped viruses that have become promising vehicles for gene therapy. However, despite the increasing number of approved AAV-based therapies, several challenges remain in the production and purification process. The need to separate full particles (containing ssDNA) from empty particles (lacking nucleic acid cargo) is particularly important.
In this application note, we present a serotype-independent strategy for optimizing empty/full separation using Sartobind® Lab anion exchange (AEX) chromatography units. For routine separations, the optimized process can be performed equipment-free, using just a syringe and appropriate buffers, eliminating the need to set up a liquid chromatography system.
Document type: Application Note
Page count: 8
Read time: 12 minutes
Last updated: September 2024
Authors: Frederik Meierrieks, Benjamin Graf, John S Cashman
Key Takeaways
- Learn how to optimize the empty/full separation for AAV samples on Sartobind® Q Lab
- See how different buffer systems and elution gradients affect the resolution between empty and full particles
- Understand how the optimized separation process can be applied in equipment-free workflows
- View data collected for AAV8, AAV5 and AAV2, demonstrating how this strategy can be applied to any AAV serotype
Audience
- Laboratory researchers
- Viral Vector Scientists
- Purification Scientists
- Downstream Process Development Scientists
Applications
- AAV Purification
- Capsid Enrichment
- Empty/Full Separation
- Ion Exchange Chromatography
- Viral Particle Fractionation
Preview of Application Note
Figure 1: Establishing a Small Scale AAV Empty/Full Separation Process using Sartobind® Q Lab cover image