Application Note: Optimization of Protein Purification on Sartobind® Lab Membrane Adsorbers
Authors: Dr. Andreas Pickl, Dr. John Cashman | Last updated: April 27, 2018
Overview
Knowing that your chromatography process has been optimized ensures less time purifying proteins, so you can focus on the experiments needed to progress your research.
Switching from resin-based columns to Sartobind® membrane adsorbers enables plug-and-play optimization and flexible handling options, while virtually eliminating the effect of flow rate on binding capacity. This minimizes hands-on time, equipment demand and optimization effort, while shortening the preparative purification process when scaling up.
- Document type: Application Note
- Page count: 4
- Read time: 4 minutes
Key Takeaways
In this application note, we outline:
- Key considerations when optimizing ion exchange purification
- Effect of ionic strength on target molecule capture and recovery
- Ability to concentrate target molecules on Sartobind® Lab IEX units
- Binding capacities and optimal pH ranges for IEX purification of selected test molecules
This Resource is Designed for:
- Researchers in laboratories expressing and purifying proteins for structural and functional studies
Applications Supported:
- Protein purification
- Ion exchange chromatography
- Anion exchange chromatography
- Cation exchange chromatography
