Using Centrifugal Ultrafiltration to Efficiently Concentrate AAV8 | Application Note
Authors: Caroline Trebini, Jannifer Labisch, John S Cashman, Klaus Schoene | Last updated: February 2025
Overview
Adeno-associated viruses (AAV) are important vectors in gene therapy, known for their efficient delivery of genetic material without eliciting a strong immune response. The purification workflow for AAVs requires capsids to be concentrated to a sufficient level for cell-based assays, analytics, and other applications. Ultrafiltration using spin filters is a convenient approach to concentrating capsids from low starting sample volumes but should be optimized to maximize AAV yield. In this study, we assessed the performance of three different spin filters to concentrate AAV8. Based on the shortest process times and highest recovery rates, we have demonstrated that Vivaspin® Turbo with 50 kDa MWCO PES membranes provided optimal results in an AAV concentration process.
- Document type: Application Guide
- Page count: 4
Read time: 6 min
Key Takeaways
- See how the results of a sample concentration can vary between spin filters from different suppliers
- Understand that using 50 kDa PES membranes can optimize your AAV purification process
- Appreciate the 40-45% time saving that is possible by switching to Vivaspin® Turbo ultrafilters
- Recognize that AAV8 yields can be improved by opting for the higher recovery rates of Vivaspin® Turbo
This Resource is Designed for:
- Laboratory Researchers
- Viral Vector Scientists
- Purification Scientists
- Downstream Process Development Scientists
Applications Supported:
- AAV Purification
- Capsid Concentration
- Centrifugal Ultrafiltration
Using Centrifugal Ultrafiltration to Efficiently Concentrate AAV8
