Application Note: From Mammalian Cell Cultures to Pure Proteins: Sartoclear Dynamics® Lab Significantly Reduces Cell Harvest Time
In this study, the novel Sartoclear Dynamics® Lab V Kit was evaluated for the removal of transiently IgG expressing mammalian MEXi-293E (HEK293) cells from cell cultures. The method was directly compared to the present standard method that required two centrifugation steps. After clarification, recombinant IgG harboring a Twin-Strep-tag® was purified from all samples in parallel by a one-step Strep-Tactin®XT Superflow® high capacity affinity purification process. For more information, download the application note below.