Centrifuge-Free Clarification of Antibodies from Cell Cultures Using Sartoclear Dynamics® Lab Decimates Working Time

A Comparative Study

This study focuses on the clarification of 50 mL suspension adapted HEK-293-6E cells, transiently expressing human IgG4 by comparing a conventional harvest method and the new Sartoclear Dynamics® Lab method. While the conventional method comprises a centrifugation step and a filtration step by using Steriflip®, the Sartoclear Dynamics® Lab method using diatomaceous earth as a filter aid in combination with either a 150 mL or a 250 mL bottle-top filter. For more information about this study, fill out the form below to download the application note. 

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