Scalable Process Chromatography Across the Entire Drug Lifecycle
Chromatography Systems, Columns, and Consumables: All From One Trusted Supplier
Unlock the full potential of your bioprocess with innovative chromatography solutions. Whether you're working with small or large molecules, in batch or continuous mode, our systems, columns, resins, membranes, and monoliths deliver scalable, high-performance purification. Designed for flexibility and efficiency, our technologies support process intensification activities from development to commercial manufacturing.
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Driving Innovation in Process Chromatography
Sartorius brings decades of expertise to process chromatography, delivering innovative solutions for both batch and continuous purification. With over 30 years of experience in bioseparation, we support biopharmaceutical and pharmaceutical companies worldwide. Our portfolio includes advanced resins, membranes, monoliths, chromatography columns, and systems designed for scalability, efficiency, and regulatory compliance, from clinical scale to commercial production.
- Trusted by Leaders: A partner to top global biopharma firms across all phases of drug development.
- Proven at Scale: Technologies used in the commercial production of mAbs, vaccines, insulin, oligonucleotides, and gene therapy molecules, such as lentiviruses.
- Intensification Ready: Pioneers in multicolumn continuous chromatography with Resolute® BioSMB and Resolute® BioSC platforms.
- Integrated Solutions: End-to-end workflow support—from resin or membrane to system—backed by global service and automation expertise.
- Versatile Chromatography Media: Solutions for mAbs, small molecules, and gene therapy applications, implemented in commercial processes.
- Innovative Ligands: Advanced ligands for purifying novel molecules and enhancing commercialized downstream processes.
Related Assets
Sartobind® Rapid A as an Efficient, Cost-Effective Alternative to Traditional Protein a Resin Chromatography for Smaller-Scale mAb Production
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Integrated | Connected Downstream Processing of mAbs With Resolute® BioSC for Improved Productivity, Cost, Footprint, and Facility Utilization
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A New Way to Perform mAb Capture: Scaling Rapid Cycling Membrane Chromatography From Bench to Commercial Manufacturing
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Re-Imagining Chromatography - Building Efficient, Connected Downstream Processes
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Implementing Chromatographic Methods for Evaluating Large-Scale Monolithic Columns for AAV Capsid Separation
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Comparability Study of an Ion Exchange Monolith and Affinity Resin for the Purification of AAV8
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How DISCOVER® Surveys Optimize End-to-End Processes to Reduce Costs and Improve Yields
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Proof of Concept for MAbs Purification: From Batch to Intensified and Connected Process
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Frequently Asked Questions
In biopharmaceutical manufacturing, process chromatography is a key technique used for the purification of biomolecules such as mAbs, vaccines, nucleic acids, and polypeptides. It employs chromatographic methods to separate these target molecules from complex mixtures, including contaminants generated during cell culture.
The mixture passes through a stationary phase, such as a resin bed, membrane, or monolith. Different components interact differently with the stationary phase, leading to their separation based on properties such as size, charge, hydrophobicity, or other characteristics. A downstream process typically includes one or more chromatographic steps.
Process chromatography is essential to producing high-purity biopharmaceuticals and represents a critical step in ensuring product quality and patient safety.
- Affinity Chromatography: Leverages specific binding interactions between a molecule and a ligand (e.g., antigen-antibody, enzyme-substrate) to selectively separate the target molecule from contaminants. It is highly selective and often used for purifying proteins and antibodies.
- Ion Exchange Chromatography: Separates molecules based on charge by using positively or negatively charged stationary phases. It is widely used in intermediate polishing steps of therapeutic proteins and for the purification of nucleic acids.
- Hydrophobic Interaction Chromatography (HIC): Separates molecules based on hydrophobicity. It is often used to purify proteins and peptides with strong hydrophobic regions.
- Mixed-Mode Chromatography: Combines different separation principles, such as ion exchange and hydrophobic interactions, to achieve improved selectivity and purification. It is used for complex biomolecule purification.
Other types of chromatography, such as size-exclusion or reverse-phase chromatography, are used less frequently in bioprocessing.
Both process chromatography and analytical chromatography use chromatographic techniques to separate biomolecules, but they serve different purposes and are used in different contexts:
- Process Chromatography is primarily used for bulk purification of biomolecules, handling larger volumes of material. These unit operations focus on separation, producing purified product ready for further processing.
- Analytical Chromatography is used to analyze the composition of a sample, identifying and quantifying the components present. It operates at a much smaller scale, with a focus on precisely detecting and measuring the components of a mixture, typically for research or quality control purposes.
Continuous chromatography is a specific separation technique, while process intensification is a broader concept that encompasses various strategies to improve the efficiency and sustainability of bioprocesses.
