Monolithic Chromatography
Process improvement with monolithic chromatography
We designed our convective interaction media (CIM®) monoliths as unique, single-unit structures – making them ideal for purifying very large biomolecules with a very small lab footprint. Large molecules are commonly used in modern gene therapy and vaccine products, and include virus particles, vesicles, RNA, plasmids, and other forms of DNA. These molecules require dedicated solutions for chromatographic purification.
Our CIM® monoliths are ideal for preparative and analytical applications, as well as high-throughput purification screening.
Monolithic Chromatography Solutions
- Higher resolution applications: High resolution capabilities in combination with low shear flow properties for quality yields
- Enhanced productivity: Increased throughput from improved yields and faster run times, benefitting from high capacity and ready-to-use formats
- Scalable plug-and-play format: Ready-to-use cGMP formats with proven scalability from 1 mL to 40 L
- Ideal for large molecule applications: A robust tool for the purification of biomolecules in the nanometer range
CIM® Monolithic Chromatography in 3D
Large biomolecules are becoming increasingly popular in the biotherapeutic industry. To purify these new modalities, we need innovative solutions like monolithic chromatography.
Join us on a journey through the CIM® monolith matrix to discover how convective chromatography supports the robust separation of large biomolecules.
Monolithic Chromatography Portfolio
Monoliths by Chemistry and Application
| IEX | HIC | Affinity | Mixed-Mode |
|---|---|---|---|
| CIMmultus® QA | CIMmultus® OH | CIMmultus® Oligo-dT | CIMmultus PrimaS® |
| CIMmultus® QA HR | CIMmultus® C4-HLD | CIMmultus PrimaS® HR | |
| CIMmultus® DEAE | CIMmultus® PrimaT | ||
| CIMmultus® SO3 | CIMmultus® Swiper | ||
| CIMmultus® SO4 | |||
| Select Your Product | Select Your Product | Select Your Product | Select Your Product |
Faster High-Capacity Purification
CIM® monoliths contain highly cross-linked, porous poly-methacrylate with well-defined channel size distribution. High surface accessibility of binding sites allows capacities beyond resin-based columns for large biomolecules, as well as rapid convection-based mass transfer.
With flow-independent performance, low back-pressure, and versatility, our monolithic columns enable you to purify viruses and nucleic acids faster. CIM® monoliths are available in a variety of chemistries and sizes ranging from screening to commercial processing for higher yields and improved speed.
CIM® Monoliths for IEX Chromatography
IEX Monolithic Chromatography
Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers designed to purify large biomolecules.
| CIMmultus® SO3 | CIMmultus® QA | CIMmultus® QA HR | CIMmultus® DEAE | |
|---|---|---|---|---|
| Channel sizes | 1.3 | 2 | 6 µm | 1.3 | 2 | 6 µm | 2 µm | 1.3 | 2 | 6 µm |
| Column volume | 1 mL – 8 L | 1 mL – 8 L | 1 mL – 8 L | 1 mL – 8 L |
| Working range, pH | 2 – 13 | 2 – 13 | 2 – 13 | 2 – 9 |
| Functionality | Strong cation exchange | Strong anion exchange | Strong anion exchange | Weak anion exchange |
| Typical application | Viruses (e.g., AAV, Flu, NDV, ORF) and VLPs | Viruses (e.g., AAV, Lenti, Adeno), VLPs, and plasma proteins | AAV | pDNA, Lenti and plasma proteins |
| View Products | View Products | View Products | View Products |
CIMmultus® SO3 – Strong CEX
- Strong cation exchanger
- Working range, pH 2–13
- Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for purification of virus and virus-like particles
- Sanitizable with 1 M NaOH
CIMmultus® QA – Strong AEX
- Strong anion exchanger
- Working range, pH 2–13
- Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for virus particles, IgM, and exosomes
- High-efficiency removal of endotoxin and DNA
- Designed for preparative applications or analytical fingerprinting of process samples
- Sanitizable with 1 M NaOH
CIMmultus® QA HR – Strong AEX
- Strong anion exchanger
- Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
- Use for polishing steps in either bind-elute or flow-through mode
- Column release criteria based on the AAV8 elution profile
- Selected to meet the need for highly reproducible purification processes (e.g., separation of AAV empty from AAV full capsids)
- Sanitizable with 0.5 M NaOH
CIMmultus® DEAE – Weak AEX
- Weak anion exchanger
- Working range, pH 2–9
- Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
- Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
- High capacity for plasmid DNA
- Sanitizable with 1 M NaOH
Monoliths for HIC
The Choice for Hydrophobic Interaction Chromatography
Hydrophobic interaction chromatography (HIC) is a must for nucleic acid separations. When combined with the advantages of monolithic chromatography, HIC meets this need while providing an excellent solution for purifying large biomolecules, such as viruses, and polishing nucleic acids (pDNA and mRNA).
| CIMmultus® OH | CIMmultus® C4-HLD | |
| Channel sizes | 1.3 | 2 | 6 µm | 2 | 6 µm |
| Column volume | 1 mL – 8 L | 1 mL – 8 L |
| Working range, pH | 2 – 10 | 2 – 10 |
| Functionality | Weak hydrophobic | Strong hydrophobic |
| Typical application | Viruses (e.g., AAV) and VLPs, LNP | Nucleic acids (pDNA, mRNA) |
| View Products | View Products |
CIMmultus® OH
- Uncharged (neutral) ligand
- Working range pH: 2 - 10
- Retains very large solutes (virus particles, VLPs, vesicles) in the presence of precipitating salts or polyethylene glycol
- Elutes large solutes with high resolution in order of increasing size
- Elution with descending salt or polyethylene glycol
- Sanitizable with 1 M NaOH
CIMmultus® C4-HLD
- Strongly hydrophobic (uncharged) ligand
- Working range pH: 2 - 10
- Highly effective for removing proteins from nucleic acids (pDNA, RNA)
Monoliths for Affinity Purification
Highly Specific Monolithic Affinity Purification
Monolithic columns combined with unique affinity ligands offer a robust solution for affinity purification of large biomolecules.
- Channel sizes: 2 µm
- Column volume: 1 mL – 40 L
- Working range pH: 2 - 13
- Functionality: Thymine oligomer-based affinity
- Application: mRNA
CIMmultus® Oligo dT – Affinity for RNA
- Oligo dT ligands covalently bound to solid support
- Hybridizes to polyadenylated tail found on most eukaryotic mRNAs, or synthesized onto the molecule during IVT
- Fast and efficient capture and purification of mRNA with a polyadenylated tail, from various sources
- Selectively removes impurities from samples
- Convenient approach for initial purification of mRNA
- Available with:
Mixed-Mode Monolithic Columns
Unique Selectivity with Mixed Mode Monolithic Columns
The unique selectivity of mixed-mode monolithic columns ensures effective purification of the most challenging large biomolecules.
| CIMmultus ® Swiper | CIMmultus PrimaS® | CIMmultus PrimaS® HR | CIMmultus® PrimaT | CIMmultus® SO4 | |
|---|---|---|---|---|---|
| Channel sizes | 2 µm | 2 µm | 2 µm | 2 µm | 2 µm |
| Column volume | 1 mL – 8 L | 1 mL – 8 L | 1 mL – 8 L | 1 mL – 800 mL | 1 mL – 40 L |
| Working range, pH | 2 – 11 | 2 – 13 | 2 – 11 | 4.5 – 13 | 4 – 13 |
| Functionality | Multimodal: anion exchange, hydrogen bonding | Hydrogen bonding and anion exchange | Multimodal: anion exchange, hydrogen bonding | Multimodal: anion exchange, hydrogen bonding, metal affinity coordination | Strong cation exchange, hydrophobic, hydrogen bonding |
| Application | mRNA | mRNA | AAV | Viruses (e.g., AAV) and bacteriophages | Plasma proteins |
| View Products | View Products | View Products | View Products | View Products |
CIMmultus ® Swiper
- Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
- Offers versatile RNA purification for various modalities
- Presents a valuable tool for the purification of non-polyadenylated RNA
- Ensures high recovery rates, typically above 80%
- Tailored for sensitive RNA constructs, ensuring optimal performance and integrity
- Purification conducted under mild loading conditions and neutral pH elution
CIMmultus® PrimaS
- Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography
- Unique selectivities in many important product areas
- Supports purification of single-stranded RNA at ambient temperature
- Fractionates ssRNA by size
CIMmultus PrimaS® HR
CIMmultus PrimaS® HR is a new generation of pre-packed chromatographic monolithic products developed to meet the most demanding separation and purification needs. HR stands for high reproducibility between batches and different scales.
- Multimodal chromatography ligand that combines elements of hydrogen bonding with weak anion exchange chromatography
- Selected to meet the need for highly reproducible purification processes (e.g., separation of full AAV capsids from empty AAV capsids)
- Column release criteria based on the AAV8 elution profile
- AAV binding at neutral or closer to neutral pH compared to QA-based ligands: Multimodal properties of CIMmultus PrimaS® HR allow binding of AAV in neutral or closer to neutral pH values compared to QA-based ligands
- cGMP compliant and suitable for clinical and commercial production
CIMmultus® PrimaT
CIMmultus® PrimaT is a multimodal chromatography column designed for the purification of large biomolecules, such as AAV and bacteriophages.
- Multimodal chromatography ligand that combines elements of hydrogen bonding with weak anion exchange chromatography and metal affinity coordination
- Provides high viral vector genome recovery, robust full AAV enrichment, and efficient hcDNA and protein clearance across a range of scales
- Multimodal properties enable AAV binding at neutral or near-neutral pH values
- Supports separation of different AAV subpopulations
- Enables superior endotoxin removal
CIMmultus® SO4
- Multimodal: Selective binding of proteins at moderate to high ionic strength (100–400 mM NaCl).
- Scalable: Proven at industrial scale (IgG) with superior FXIa removal. Strict and unified column release criteria across scales together with flow-independent performance, ensure high quality columns and consistent resolution and capacity regardless of column batch and scale.
- Gentle and rapid: Exclusively laminar flow in CIM monoliths minimizes shear, leading to improved recovery of physiologically active components (IgG, coagulation factors). Flow-independent column performance and high back-pressure tolerance enable rapid purification cycles with flow rates up to 2,400 L/h.
- High reusability: Supports cost-effective, affordable purification of IgG and coagulation factors.
Monolith Process Packs
Process packs kick-start your purification process
Process packs support the development and scale-up of large molecule purification. They come with recommended CIMmultus® columns and detailed process instructions to achieve high-quality purification in a short time.
HiP² Plasmid Process Pack is a ready-to-use, adjustable pDNA purification pack designed to help you develop your own pDNA purification process and transfer it to production.
Your process pack comes with detailed experiments to help you save time and money.
The process is extremely flexible, and you can pick and choose parts of the protocol to tailor it to your needs. CIMmultus® HiP² Plasmid Process Pack contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) column, and a purification protocol of your plasmid.
| HiP2 Plasmid Process Pack | |
| Column volume | 1 – 80 mL |
| Included chemistries | CIMmultus® DEAE CIMmultus® C4-HLD |
| Additional components | Detailed process instructions |
| View Products |
Chromatography Screening
Automated Precision with CIM® Octa Columns
Enhance your process development and screening workflow with efficiency and versatility tailored to your bioprocess needs. You can easily transfer methods developed on CIM® Octa columns to other monolith formats. With plug-and-play and multi-use potential, CIM Octa columns provide a robust solution for both research and industrial applications.
Condition Screening and HTP Purification with CIM® Monolithic Well Plates
We coupled CIM monolith technology with a standard multi-well plate design. Whether your goal is to screen multiple chromatographic conditions at once or high-throughput purification of your target molecule, our CIM plates offer a solution in either a 24-well or 96-well plate design.
Learn More About Monolithic Chromatography
Highlight asset
Related assets
Revolutionizing AAV8 Purification: Scalable Two-Step Chromatography Process
PDF | 1.1 MB
Miniaturisation of Trypsin Immobilised Enzymatic Reactor for Protein Digestion
PDF | 314.1 KB
Increasing Productivity of pDNA DSP Using Sample Displacement Chromatography
PDF | 459.9 KB
Recovery of pDIKE2 Plasmid for Hepatitis C Vaccine Using CIMmultus® C4 HLD Monolith
PDF | 338.5 KB
Implementing Chromatographic Methods for Evaluating Large-Scale Monolithic Columns for AAV Capsid Separation
PDF | 531.9 KB
Comparability Study of an Ion Exchange Monolith and Affinity Resin for the Purification of AAV8
PDF | 2.7 MB
