Monolithic Chromatography

Process Improvement With Monolithic Chromatography

CIM® (Convective Interaction Media) monoliths are designed as unique, single-unit structures – making them ideal for purifying very large biomolecules with a very small lab footprint. Large molecules are used commonly in modern gene therapy and vaccines products, and include virus particles, vesicles, RNA, plasmids, and other forms of DNA. These molecules require dedicated solutions for chromatographic purification.

The Sartorius family of CIM® monoliths is ideal for preparatory and analytical applications as well as immobilization screening. Pre-configured product packs are also available, a combination of columns and a protocol to suit specific needs.


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Higher resolution applications

High resolution capabilities in combination with low shear flow properties for quality yields

Enhanced productivity

Increased throughput from improved yields and faster run times, benefitting from high capacity and ready-to-use formats

Scalable plug-and-play format

Ready-to-use cGMP formats with proven scalability from 1 mL to 40 L

Ideal for large molecule applications

A robust tool for the purification of biomolecules in the nanometer range 

Monoliths by Chemistry and Application

A Selection of Monoliths By Chemistry

IEX

HIC

Affinity

Mixed-Mode

CIMmultus® QA 

CIMmultus® OH 

CIMmultus® Oligo-dT 

CIMmultus® PrimaS 

CIMmultus® DEAE 

CIMmultus® C4-HLD 

CIMmultus® H-Bond 

CIMmultus® EV 

CIMmultus® EDA 

CIMmultus® SO3 

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Choose Your Product

Choose Your Product

Choose Your Product

Monolithic Chromatography Across Multiple Applications

AAV & Other Viral Vectors 

mRNA 

pDNA 

Exosomes 

CIMmultus® OH

CIMmultus® Oligo dT

CIMmultus® DEAE

CIMmultus® EV

CIMmultus® SO3

CIMmultus® PrimaS 

CIMmultus® C4-HLD

Cornerstone® Exosome
Process Development Pack

CIMmultus® QA

CIMmultus® C4-HLD

HiP2 Plasmid Process Pack

 

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Faster High-Capacity Purification

CIM® monoliths contain highly cross-linked, porous poly-methacrylate with well-defined channel size distribution. High surface accessibility of binding sites allows capacities beyond resin-based columns for large biomolecules, as well as rapid convection-based mass transfer.

With flow-independent performance, low back-pressure, and versatility our monolithic columns facilitate faster purification of your viruses or nucleic acids. CIM® monoliths are available in a variety of chemistries and sizes ranging from screening to commercial processing for higher yields and improved speed.

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Analytical Chromatography Columns

Provide information in real-time, through at-line monitoring of large biomolecules including viruses, pDNA & mRNA.

Analytical Chromatography
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Cornerstone® Biomanufacturing Development Services

Sartorius experts represent extensive experience in chromatography and purification of biomolecules as well as analytical methods development.

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Monoliths for IEX Chromatography

IEX Monolithic Chromatography

Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.
 

CIMmultus® SO3

CIMmultus® QA

CIMmultus®
DEAE

CIMmultus® EDA

CIMmultus® EV

Channel sizes 

1.3 | 2 | 6 µm 

1.3 | 2 | 6 µm

1.3 | 2 | 6 µm

2 µm

2 µm

Column volume 

1 mL – 8 L

1 mL – 8 L

1 mL –8 L

1 mL – 8 L

1 mL – 8 L

Working range, pH 

2 – 13

2 – 13

2 – 9

2 – 9

2 – 13

Functionality 


Strong cation exchange 

Strong anion exchange 

Weak anion exchange 

Weak anion exchange 

Strong anion exchange 

Typical application 


Viruses, (i.e. AAV),
and VLPs

Viruses, (i.e. AAV),
and VLPs

pDNA

pDNA

Exosomes

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CIMmultus® SO3 – Strong CEX

  • Strong cation exchanger

  • Working range, pH 2–13

  • Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode

  • High capacity for purification of virus and virus-like particles

  • Sanitizable with 1 M NaOH

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CIMmultus® QA – Strong AEX

  • Strong anion exchanger 

  • Working range, pH 2–13 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for virus particles, IgM, exosomes 

  • High-efficiency removal of endotoxin and DNA 

  • Outstanding for preparative applications or analytical fingerprinting of process samples 

  • Sanitizable with 1 M NaOH

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CIMmultus® DEAE – Weak AEX

  • Weak anion exchanger 

  • Working range, pH 2–9 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for plasmid DNA 

  • Sanitizable with 1 M NaOH

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CIMmultus® EDA – Weak AEX & Activated Group

  • Weak anion exchanger 

  • Working range, pH 2–9 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for plasmid DNA 

  • Sanitizable with 1 M NaOH

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CIMmultus® EV – Strong AEX

  • Strong anion exchanger 

  • Working range, pH 2–13 

  • High capacity for exosomes 

  • High-efficiency reduction of host cell DNA 

  • High efficiency removal of viruses, endotoxins, and non-exosomal vesicles 

  • Sanitizable with 1 M NaOH

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Monoliths for HIC

The Choice for Hydrophobic Interaction Chromatography (HIC)

Hydrophobic interaction chromatography (HIC) is a must for nucleic acid separations. When combined with the advantages of monolithic chromatography, HIC meets this need while also providing an excellent solution for the purification of large biomolecules including adenoassociated viruses (AAV).


CIMmultus® OH

CIMmultus® C4-HLD

Channel sizes 

1.3, 2, 6 µm

2, 6 µm

Column volume 

1 mL – 8 L

1 mL – 8 L

Working range, pH 

2 – 10

2 – 10

Functionality 

Weak hydrophobic

Strong hydrophobic

Typical application 

Viruses, (i.e. AAV), and VLPs 

Nucleic acids (pDNA, mRNA) 

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CIMmultus® OH – Hydrophobic Interaction Chromatography

  • Uncharged (neutral) ligand

  • Working range, pH 2–10

  • Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol

  • Elutes large solutes with high resolution in order of increasing size

  • Elution with descending salt or polyethylene glycol

  • Sanitizable with 1 M NaOH

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CIMmultus® C4-HLD - Hydrophobic Interaction Chromatography

  • Strongly hydrophobic, (uncharged) ligand

  • Working range, pH 2–10

  • Highly effective for removing proteins from nucleic acids (pDNA, RNA)

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Monoliths for Affinity Purification

Highly Specific Monolithic Affinity Purification

Monolithic columns combined with unique affinity ligands offer an unmatched solution for affinity purification of large biomolecules.

  • Channel sizes:  2 µm
  • Column volume: 1 mL – 8 L
  • Working range, pH: 2 - 13
  • Functionality: Thymine oligomer based affinity
  • Application:  mRNA

CIMmultus® Oligo dT – Affinity for RNA

  • Oligo dT ligands covalently bound to solid support

  • Hybridizes to poly-adenylated tail found on most eukaryotic mRNAs, or synthetized onto the molecule during IVT

  • Fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources

  • Selectively removes impurities from samples

  • Convenient approach for initial purification of mRNA

  • Available with:


Mixed Mode Monolithic Columns

Unique Selectivity with Mixed Mode Monolithic Columns

The unique selectivity of mixed-mode monolithic columns ensures the proper purification of the most challenging large biomolecules.


CIMmultus® PrimaS

CIMmultus® H-Bond

Channel sizes 

2 µm

1.3, 2 µm

Column volume 

1 mL – 8 L

1 mL – 8 L

Working range, pH

2 – 13

2 – 13

Functionality 

Hydrogen bonding and anion exchange

Multimodal hydrogen donor-acceptor

Application

mRNA

Viruses and extra-cellular vesicles

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CIMmultus® PrimaS

  • Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography 

  • Unique selectivities in many important product areas 

  • Supports purification of single-stranded RNA at ambient temperature

  • Fractionates ssRNA by size

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CIMmultus® H-Bond ADC

  • Multimodal hydrogen donor-acceptor

  • Working range, pH 2–13

  • Enables size-based separation of product and contaminants at acidic pH

  • Elution with increasing salt, pH, or sorbitol

  • Highly tolerant of salt

  • Outstanding DNA removal

  • Unique selectivity for preparative and analytical applications

  • Sanitizable with 1 M NaOH

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Monolith Process Packs

Process Packs Kick-Start Your Purification Process

Process Packs support the development and scale-up of large molecule purification. They come with recommended CIMmultus® columns and detailed process instructions to achieve high-quality purification in a short time.


HiP2 Plasmid Process Pack

Cornerstone® Exosome Process Development Pack

Column volume

1 – 80 mL

1 mL (larger on request)

Included chemistries

CIMmultus® DEAE

CIMmultus® C4-HLD

CIMmultus® EV

Additional components

Detailed process instructions

Kryptonase DNAse

Detailed process instructions

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HiP²  Plasmid Process Pack

HiP²  Plasmid Process Pack is a ready-to-use, adjustable pDNA purification pack, to help develop your own pDNA purification process and transfer it to production.

Detailed experiments are included, saving save time and money.

The process is extremely flexible, and allows choosing only parts of the protocol that fit your needs. CIMmultus® HiP² Plasmid Process Pack contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) column and a purification protocol of your plasmid.


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Cornerstone® Exosome Process Development Pack

Cornerstone® Exosome Process Development Pack is a ready-to-use, adjustable exosome purification pack to help you develop your own purification process and transfer to production.

Detailed experiments are included, saving time and reducing cost. Cornerstone® Exosome Process Development Pack contains enzyme-based reagent (Kryptonase), one ion-exchange (EV) 1 mL column, and a purification protocol of your sample.

For bigger volumes, please contact our sales team.


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