Monolithic Chromatography

Process Improvement With Monolithic Chromatography

CIM® (Convective Interaction Media) monoliths are designed as unique, single-unit structures – making them ideal for purifying very large biomolecules with a very small lab footprint. Large molecules are used commonly in modern gene therapy and vaccines products, and include virus particles, vesicles, RNA, plasmids, and other forms of DNA. These molecules require dedicated solutions for chromatographic purification.

The Sartorius family of CIM® monoliths is ideal for preparatory and analytical applications as well as immobilization screening. Pre-configured product packs are also available, a combination of columns and a protocol to suit specific needs.


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Monolithic Chromatography in 3D

Large biomolecules are becoming increasingly popular in the biotherapeutic industry. Innovative solutions - such as monolithic chromatography - are needed to purify these new modalities.  

Join us on a journey through the monolith matrix to discover how convective chromatography supports the robust separation of large biomolecules.

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Webinar: Why you need monoliths to purify your biomolecules

Watch our webinar to learn what makes monoliths ideal for the challenges of purifying large biomolecules. 

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Higher resolution applications

High resolution capabilities in combination with low shear flow properties for quality yields

Enhanced productivity

Increased throughput from improved yields and faster run times, benefitting from high capacity and ready-to-use formats

Scalable plug-and-play format

Ready-to-use cGMP formats with proven scalability from 1 mL to 40 L

Ideal for large molecule applications

A robust tool for the purification of biomolecules in the nanometer range 

Chromatography Screening

Automated precision in Chromatography Screening

Enhance your process development and screening workflow with the efficiency and versatility tailored to your bioprocess needs. Methods developed on CIM® Octa can be easily transferred to other monolith formats. With plug-and-play and multi-use potential, CIM Octa columns provide a robust solution for both research and industrial applications.

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Screening of Chromatographic Conditions and HTP Purification

We coupled CIM monolith technology with a standard multi-well plate design. Whether your goal is to screen multiple chromatographic conditions at once or high throughput purification of your target molecule, our CIM plates offer an answer in either a 24-well or 96-well plate design.

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Monoliths by Chemistry and Application

A Selection of Monoliths By Chemistry

IEXHICAffinityMixed-Mode
CIMmultus® QA CIMmultus® OH CIMmultus® Oligo-dT CIMmultus PrimaS®
CIMmultus® QA  HRCIMmultus® C4-HLD  CIMmultus H-Bond®
CIMmultus® DEAE   
CIMmultus® EDA    
CIMmultus® SO3    
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Faster High-Capacity Purification

CIM® monoliths contain highly cross-linked, porous poly-methacrylate with well-defined channel size distribution. High surface accessibility of binding sites allows capacities beyond resin-based columns for large biomolecules, as well as rapid convection-based mass transfer.

With flow-independent performance, low back-pressure, and versatility our monolithic columns facilitate faster purification of your viruses or nucleic acids. CIM® monoliths are available in a variety of chemistries and sizes ranging from screening to commercial processing for higher yields and improved speed.

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Analytical Chromatography Columns

Provide information in real-time, through at-line monitoring of large biomolecules including viruses, pDNA & mRNA.

Analytical Chromatography
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Cornerstone® Biomanufacturing Development Services

Sartorius experts represent extensive experience in chromatography and purification of biomolecules as well as analytical methods development.

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Monoliths for IEX Chromatography

IEX Monolithic Chromatography

Benefit from all the attributes of monolithic chromatography with a range of strong and weak anion and cation exchangers to meet the needs for purification of large biomolecules.

 CIMmultus® SO3CIMmultus® QACIMmultus® QA HRCIMmultus® DEAECIMmultus®  EDA
Channel sizes 1.3 | 2 | 6 µm 1.3 | 2 | 6 µm2 µm1.3 | 2 | 6 µm2 µm
Column volume 1 mL – 8 L1 mL – 8 L1 mL – 8 L1 mL –8 L1 mL – 8 L
Working range, pH 2 – 132 – 132 – 132 – 92 – 9

Functionality 

 

Strong cation exchange Strong anion exchange Strong anion exchange Weak anion exchangeWeak anion exchange 

Typical application 

 

Viruses, (i.e. AAV),
and VLPs
Viruses, (i.e. AAV),
and VLPs
AAVpDNApDNA
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CIMmultus® SO3 – Strong CEX

  • Strong cation exchanger

  • Working range, pH 2–13

  • Binds molecules with predominantly positive charge, repels molecules with predominantly negative charge

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode

  • High capacity for purification of virus and virus-like particles

  • Sanitizable with 1 M NaOH

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CIMmultus® QA – Strong AEX

  • Strong anion exchanger 

  • Working range, pH 2–13 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for virus particles, IgM, exosomes 

  • High-efficiency removal of endotoxin and DNA 

  • Outstanding for preparative applications or analytical fingerprinting of process samples 

  • Sanitizable with 1 M NaOH

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CIMmultus® QA HR – Strong AEX

  • Strong anion exchanger
  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge
  • Use for polishing steps in either bind-elute or flow-through mode
  • Column release criteria based on the AAV8 elution profile
  • Selected to meet the need for highly reproducible purification processes (e.g., separation of AAV empty from AAV full capsids)
  • Sanitizable with 0.5 M NaOH

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CIMmultus® DEAE – Weak AEX

  • Weak anion exchanger 

  • Working range, pH 2–9 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for plasmid DNA 

  • Sanitizable with 1 M NaOH

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CIMmultus® EDA – Weak AEX & Activated Group

  • Weak anion exchanger 

  • Working range, pH 2–9 

  • Binds molecules with predominantly negative charge, repels molecules with predominantly positive charge 

  • Capture, intermediate, or polishing steps in either bind-elute or flow-through mode 

  • High capacity for plasmid DNA 

  • Sanitizable with 1 M NaOH

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Monoliths for HIC

The Choice for Hydrophobic Interaction Chromatography (HIC)

Hydrophobic interaction chromatography (HIC) is a must for nucleic acid separations. When combined with the advantages of monolithic chromatography, HIC meets this need while also providing an excellent solution for the purification of large biomolecules including adenoassociated viruses (AAV).


CIMmultus® OH

CIMmultus® C4-HLD

Channel sizes 

1.3, 2, 6 µm

2, 6 µm

Column volume 

1 mL – 8 L

1 mL – 8 L

Working range, pH 

2 – 10

2 – 10

Functionality 

Weak hydrophobic

Strong hydrophobic

Typical application 

Viruses, (i.e. AAV), and VLPs 

Nucleic acids (pDNA, mRNA) 

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CIMmultus® OH – Hydrophobic Interaction Chromatography

  • Uncharged (neutral) ligand

  • Working range, pH 2–10

  • Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol

  • Elutes large solutes with high resolution in order of increasing size

  • Elution with descending salt or polyethylene glycol

  • Sanitizable with 1 M NaOH

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CIMmultus® C4-HLD - Hydrophobic Interaction Chromatography

  • Strongly hydrophobic, (uncharged) ligand

  • Working range, pH 2–10

  • Highly effective for removing proteins from nucleic acids (pDNA, RNA)

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Monoliths for Affinity Purification

Highly Specific Monolithic Affinity Purification

Monolithic columns combined with unique affinity ligands offer an unmatched solution for affinity purification of large biomolecules.

  • Channel sizes:  2 µm
  • Column volume: 1 mL – 8 L
  • Working range, pH: 2 - 13
  • Functionality: Thymine oligomer based affinity
  • Application:  mRNA

CIMmultus® Oligo dT – Affinity for RNA

  • Oligo dT ligands covalently bound to solid support

  • Hybridizes to poly-adenylated tail found on most eukaryotic mRNAs, or synthetized onto the molecule during IVT

  • Fast and efficient capture and purification of mRNA with a poly-adenylated tail, from various sources

  • Selectively removes impurities from samples

  • Convenient approach for initial purification of mRNA

  • Available with:


Mixed Mode Monolithic Columns

Unique Selectivity with Mixed Mode Monolithic Columns

The unique selectivity of mixed-mode monolithic columns ensures the proper purification of the most challenging large biomolecules.


CIMmultus® PrimaS

CIMmultus® H-Bond

Channel sizes 

2 µm

1.3, 2 µm

Column volume 

1 mL – 8 L

1 mL – 8 L

Working range, pH

2 – 13

2 – 13

Functionality 

Hydrogen bonding and anion exchange

Multimodal hydrogen donor-acceptor

Application

mRNA

Viruses and extra-cellular vesicles

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CIMmultus® PrimaS

  • Multimodal chromatography ligand that combines elements of hydrogen bonding with anion exchange chromatography 

  • Unique selectivities in many important product areas 

  • Supports purification of single-stranded RNA at ambient temperature

  • Fractionates ssRNA by size

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CIMmultus® H-Bond ADC

  • Multimodal hydrogen donor-acceptor

  • Working range, pH 2–13

  • Enables size-based separation of product and contaminants at acidic pH

  • Elution with increasing salt, pH, or sorbitol

  • Highly tolerant of salt

  • Outstanding DNA removal

  • Unique selectivity for preparative and analytical applications

  • Sanitizable with 1 M NaOH

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Monolith Process Packs

Process Packs Kick-Start Your Purification Process

Process Packs support the development and scale-up of large molecule purification. They come with recommended CIMmultus® columns and detailed process instructions to achieve high-quality purification in a short time.


HiP2 Plasmid Process Pack

Column volume

1 – 80 mL

Included chemistries

CIMmultus® DEAE

CIMmultus® C4-HLD

Additional components

Detailed process instructions

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HiP² Plasmid Process Pack

HiP² Plasmid Process Pack is a ready-to-use, adjustable pDNA purification pack, to help develop your own pDNA purification process and transfer it to production.

Detailed experiments are included, saving save time and money.

The process is extremely flexible, and allows choosing only parts of the protocol that fit your needs. CIMmultus® HiP² Plasmid Process Pack contains one weak anion-exchange (DEAE) and one hydrophobic (C4 HLD) column and a purification protocol of your plasmid.


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Miniaturisation of Trypsin Immobilised Enzymatic Reactor for Protein Digestion

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Recovery of pDIKE2 Plasmid for Hepatitis C Vaccine Using CIMmultus® C4 HLD Monolith

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Rapid and Effective Method for Separating Full and Empty AAV8 Particles With CIMmultus® QA Monolith 

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Implementing Chromatographic Methods for Evaluating Large-Scale Monolithic Columns for AAV Capsid Separation

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Comparability Study of an Ion Exchange Monolith and Affinity Resin for the Purification of AAV8

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Learn How to Achieve Better Vaccine Purity and Higher Yields With CIMmultus® QA Columns

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Preparation of Linear Plasmid DNA for in Vitro Transcription Reaction

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Webinars

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mRNA Production and Purification in the Aftermath of Pandemic

This webinar features an exclusive panel discussion where the speakers lay out their vision for truly optimized next-gen mRNA processes.

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Integrated Production Process of Highly Purified mRNA

Learn how a new, optimized purification approach for mRNA provides higher yields and lower costs

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