Transfection Reagents and pDNA Solutions

Transfection Reagents and Plasmid DNA

Transient transfection is a method of choice to modify the expression of specific gene(s) in eukaryotic cells. It requires three main components to create a transfection mix which are the nucleic acids (pDNA, mRNA, siRNA, etc), the transfection reagents and a complexation buffers/media.

The delivery system needs to be carefully selected based on the application and its challenges:

  • Gene and Cell Therapy rely on the use of modified viruses as vectors to transfer genes into cells that they are able to infect. Production of viral vectors is dependent on efficient transfection of one to several plasmid DNA containing the necessary viral genome to produce recombinant viral particles.
  •  RNA/DNA Therapeutics using lipid nanoparticles (LNPs), polymers or even liposomes as a delivery system to target different organs via different administration routes (in vivo) depending on the therapeutic pupose.
  • Protein Production (antibodies, peptides or proteins) that requires a screening phase using transient transfection of one or several plasmid DNA in CHO or HEK cell lines before moving to stable expression system at larger scale.
  •  Life Science Research that requires specific transfection system depending on various type of nucleic acids, cells, media and support of cell culture.

Choose among a broad range of powerful transfection reagents designed for specific application to be successful in your projects. Transfection reagents are ready-to-use with a pre-optimized protocol to ensure high transfection efficiency while preserving cell viability and morphology.

We assist academics, biotech and Pharma companies from R&D to manufacturing with end-to-end nucleic acid services, covering plasmid design, plasmid engineering, plasmid manufacturing (research to GMP), plasmid sequencing and process development and manufacturing of new biologics (viral vectors, recombinant proteins, antibody fragments, biosimilars and biobetters).

View ProductsConsult Our Experts

Highlight Products

FectoVIR®-AAV 

Available at research and GMP grade to intensify production of recombinant AAV.

Learn More

PEIpro®

A leading PEI-based DNA transfection reagent that offers flexibility and scalability  in viral vector manufacturing process.

Learn More

FectoVIR®-LV

Transfection reagent designed to improve Lentiviral vector (LV) titers in HEK-293 suspension cell systems.

Learn More

pPLUS® AAV-Helper 

 A novel plasmid helper designed for AAV production.

Learn More

pPLUS® AAV-RC2

A novel Helper plasmid for rAAV production enhancing AAV productivity, further improved through plasmid ratio optimization.

Learn More

Transfection Reagents and Plasmids

Explore Our Transfection Reagents and Plasmids

Take a look at our innovation solution that offers gold-standard products and services from research to commercialization that enhance the quality and reliability of: 


Viral Vector for CGT

The development of robust, scalable, cost-effective processes that yield high titers of high-quality viral vectors is crucial to the successful commercialization of safe and efficacious gene therapies that require large and/or multiple doses to treat more prevalent diseases. The successful commercialization of viral vector-based therapies relies on GMP-compliant and scalable, industrialized production platforms to rapidly advance high-quality products to market while also ensuring patient safety.

The most utilized approach to AAV (adeno-associated virus) and lentivirus (LV) production is through the transient transfection of mammalian cell lines, such as HEK293 or derivatives, followed by viral vector harvest, downstream purification processes and analytics for final product testing.

Since viral vector production is a significant cost driver in AAV and LV-based gene therapies, it is prudent to optimize each step of the upstream manufacturing process already at the process development phase to ensure compliance of critical raw materials (eg. plasmid DNA, transfection reagents), maximize yield and product quality while minimizing costs.

FectoVIR®-AAV 

Available at research and GMP grade to intensify production of recombinant AAV.

Learn More

FectoVIR®-LV

FectoVIR®-LV transfection reagent designed to improve Lentiviral vector (LV) titers in HEK-293 suspension cell systems.

Learn More

PEIpro®

A leading PEI-based DNA transfection reagent that offers flexibility and scalability  in viral vector manufacturing process.

Learn More

pPLUS® AAV-Helper

A novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in suspension.

Learn More

pPLUS® AAV-RC

A set of ready-to-use RepCap plasmids for AAV production of various serotypes through triple transfection. Optimized for use with pPLUS® AAV-Helper.

Learn More

Viral Vector DOE Service

Optimize your process and prepare for scale up with fewer trials.

Consult Our Experts

RNA|DNA Therapeutics

Non-viral in vivo delivery reagents are the most powerful alternative to viral vectors for nucleic acid delivery. They offer substantial advantages in terms of reliability, safety and costs for nucleic-acid based therapies. Different quality grades of in vivo transfection reagents are available for proof concept studies, preclinical studies and clinical trials.

LipidBrick® Library

A novel range of proprietary cationic lipids dedicated to the formulation of LNPs for the development of mRNA-based vaccines and therapeutics.

Learn More

in vivo-jetRNA®+

Ready-to-use transfection reagent composed of lipid-based nanoparticles specifically developed for both in vivo and ex vivo mRNA delivery.

Learn More

in vivo-jetPEI®

Ready-to-use cationic polymer reagent recommended for in vivo transfection of DNA, siRNA, miRNA, shRNA and other oligonucleotides.

Learn More

Protein Production

Transient production of recombinant protein or monoclonal antibodies is an important step in research to study the behavior of a protein of interest in a cell, to screen and candidate proteins in early stages of therapeutic research and to enable modulation of molecular mechanism in many other fields of application. 

Our goal is to help you make this protein production step as seamless as possible by optimizing the genetic engineering of your plasmid of interest and the transient expression system, whether bacterial, yeast or mammalian cell-based to meet your requirements for protein yield, function and quality.

What we can provide:

  • Solutions for transient protein production in mammalian cells: we offer ready to use cost-effective transfection solutions (FectoPRO reagent) for transient protein production in mammalian cells, mainly HEK-293 and CHO cell lines, as well as plasmid engineering service to generate any plasmid encoding for your protein of interest. 
  • CDMO protein manufacturing service in bacteria and yeast: development of production methods for recombinant proteins and manufacturing of proteins and antibody fragments to fit your needs from 100 mg to 50g (Research, GLP and GMP grade).

FectoPRO®

FectoPRO® is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells.

Learn More

FectoCHO® Expression System

FectoCHO® expression system is optimized for culture and transient protein expression of all CHO cells.

Learn More

Life Science Research

Explore our extensive range of life science delivery solutions for scientists, including ready-to-use transfection reagents suitable for most mammalian primary cells and cell lines, as well as off-the-shelf plasmids. These products ensure that you can conduct your research smoothly and rely on the following approaches:

  • A range of best-in-class transfection reagents to study the function of a protein of interest, its role in a signaling pathway, perform genome editing using plasmid-based or RNA-based proven methods, and to conduct cost-effective RNA interference to transfect guide RNA and express Cas9 protein in mammalian cells.
  • Tailored transfection solutions: Choose from our selection of specialized reagents, including jetPRIME®, INTERFERin®, and more, for targeted gene expression, genome editing, and RNA interference studies. Request samples and benefit from our scientific support to optimize your experiments and assist you during your evaluation phase.

jetPRIME®

A versatile DNA and siRNA transfection reagent that ensures high DNA transfection efficiency and excellent gene silencing.

Learn More

jetOPTIMUS®

Leverages innovative cationic nanotechnology developed to improve DNA transfection efficiency in hard-to-transfect cells.

Learn More

INTERFERin®

Achieves over 90% gene silencing in a wide variety of cells; suitable for transfecting miRNA and other oligonucleotides.

Learn More

jetMESSENGER®

An efficient and gentle mRNA transfection reagent for high mRNA transfection in primary cells, cancer cell lines, neurons, and stem cells.

Learn More

jetPEI®

A robust and reproducible transfection reagent, ideal for automated or manual high-throughput screening workflows.

Learn More

PULSin®

A powerful and efficient reagent for delivering proteins, antibodies, and peptides into mammalian cells.

Learn More

pDNA Services

Explore Our pDNA Solutions

Our pDNA services helps you to have  as much control as you want over the plasmids you use.  Allowing  you to progress with confidence in your research and ensure the level of performance you need, whether it be tissue-specific expression, high yield protein expression, RNA synthesis or high viral titers for therapeutic purposes. 

We offer tailor-made plasmids with fully flexible content, designed by you and our plasmid scientists, ready-to-use and manufactured at any scale and at any quality grade from research to GMP grade


Plasmid Engineering Service

Tailor-made DNA plasmid that really fits your needs from discovery grade to GMP grade manufacturing.

Consult Our Experts

Plasmid Manufacturing Service

Plasmid DNA manufacturing using an unique process for Research, HQ and GMP grades

Consult Our Experts

Whole Plasmid Sequencing Service

Full-lenght plasmids sequencing to provides high-quality and accurate sequencing of any plasmid.

Consult Our Experts

Easy Plasmid Service

Discover our range of easy & ready-to-use plasmids. Designed to cater to your specific needs by ensuring a seamless and cost-effective experience.

Consult Our Experts

Service Datasheets

Datasheet

Easy Plasmid Service - Plasmid Designed to Cater To Your Specific Requirements, Ensuring a Seamless And Cost-Effective Experience

PDF | 153.1 KB
Datasheet

Tailor-Made Plasmid Service - Engineering and Production Services for Plasmids From Discovery to GMP-Grade

PDF | 257.8 KB
Datasheet

Whole Plasmid Sequencing Service - Full-Length Sequencing of Multiple DNA Plasmids

PDF | 326.2 KB

Looking for Guided Support

icon-certificate
Where Can I Find Certificates Of Analysis?

You can find certificate of analysis for all product range in Sartorius Portal.  You can access them by creating an account.

Browse My.Sartorius

icon-coach
Troubleshoot Your Transfection

From starting transfection, changing plasmid DNA construct, working with a new cell line or isolated primary cells can require fine-tuning. Our transfection experts are always available to help you.
Contact Support Team

The Who, The Why and How of Transfection?

Scientists rely on transfection as a powerful technique to modulate gene expression in eukaryotic cells in vitro and in vivo. Transfection can be used from researchers in universities or research institutes to engineers in Biotechnology or Pharmaceutical companies.

Transfection is the process that allows exogenous nucleic acids to bypass the cell membrane to enter into cells. Exogenous nucleic acids commonly used are plasmid DNA, RNA, siRNA and oligonucleotides. Once delivered into cells, nucleic acids modulate gene expression by driving overexpression or silencing of a gene of interest.

Gene overexpression is an indispensable tool for several applications, from understanding the role of gene of interest (gene studies, high-throughput screening), to the production of biologics such as antibodies (protein production) and recombinant viral particles, particularly for therapeutic purposes (virus production for gene & cell therapy).

Gene silencing is a method used to prevent expression of a gene of interest. The expression of a gene can be partially reduced (gene knockdown) or completely blocked (gene knockout). Because any gene can potentially be targeted, gene silencing is a prevalent technique used to develop gene-based therapies to address monogenic pathologies, cancer and in immunotherapy strategies.

Transfection of nucleic acids is used to transiently or stably modifed cells by overexpressing or silencing specific gene(s). Several methods can be used to performed transfection that are generally divided in two different categories: Chemical and physical. There are several physical methods that exist such as electroporation, sonoporation or microinjection but these processes are complex and relatively toxic for mammalian cells. To solve these issues, chemical-mediated transfection offers a great alternative: easiness of use, high transfection efficiency and excellent cell viability. Chemical transfection are typically performed using cationic polymers or lipids that will protect the anionic nucleic acids.

  1. Encapsulation of Genetic Material with Transfection Reagent
    Nucleic acids are negatively charged due to their polyphosphate backbone and are thus able to interact with positively charged transfection reagents (polymers or lipids). This results in the formation of transfection complexes or nanoparticles, which protect nucleic acids from nuclease-mediated degradation.
  2. Cellular Uptake of Nanoparticle
    Most cells express negatively charged heparan sulfate proteoglycans on the external surface of their cell membrane, with which positively charged transfection complexes are able to interact. This interaction is key to trigger cellular uptake via an endocytosis process.
  3. Release into the Cytosol and if Needed Transport into the Nucleus for Transcription
    Upon cellular uptake, transfection complexes are sequestrated into intracellular vesicles. Our transfection reagents are able to induce the release of the nucleic acids into the cytoplasm through vesicle membrane rupture or fusion. Most nucleic acids (oligonucleotides, siRNA, mRNA, etc) stay in the cytoplasm where they are active. In case of gene transfer, plasmid DNA is transported into the nucleus for transient expression) which can become permanent after genome integration (stable expression).

More About Transfection Reagents 

Related Assets

Poster

Poster | pPLUS® AAV-Helper

Novel engineered pHelper plasmid to improve yield and quality of several AAV serotypes in suspension cell culture systems.

Download
Poster

Poster | FectoVIR®-AAV

Next-generation transfection reagent for large scale AAV manufacturing.

Download
Flyer

A Proven Combination for AAV Production - Transfection Reagent & C...

Download Now

Related Topics

Cell and Gene Therapy

Cell and gene therapy solutions driven by reproducibility, scalability, and support for regulatory compliance. 

Learn More

Polyplus Is Now Part of Sartorius

Improve process economics in cell and gene therapies.

Learn More

Cell Culture Media

Trust Sartorius for high quality cell culture media and supplements to nourish your cell line and simplify your progress.

Learn More

Consult Our Experts

Consult Our Experts

Ready to Buy?