Automated Chromatography Columns
Attribute | Resolute® Prochrom | Resolute® Prochrom | Resolute® AutoPak |
|---|---|---|---|
Scale | 150-300mm | 450-1200mm | 400-2000mm |
Application | Small to mid scale packed bed chromatography processes | Mid scale packed bed chromatrography processes | Mid to large scale packed bed chromatography processes |
Automated Operations | Semi-automatic elemental packing technology using syringe effect | Semi-automatic packing and unpacking using packing units | Fully automated packing, unpacking, CIP and in-place repacking |
Max Operating Pressure | 5 barg | 5 barg | 3 barg |
Tube Material | Stainless steel or acrylic | Stainless steel or acrylic | Stainless steel or acrylic |
Explosion Proof | Optional | Optional | No |
| Request a Quote |
Resolute® Prochrom
Resolute® Prochrom columns have been designed to run for decades without any loss of performance. This performance consistency over time is enabled by Dynamic Axial Compression (DAC) technology. Our columns are engineered for ease of cleaning and maintenance operations while maximizing operator safety.
- Efficiency and scalability - complete range from lab to industrial scale
- Fast and safe packing and unpacking - packing in 15 to 30 min maximum
- Explosion proof design when organic solvent is used
Resolute® AutoPak
Resolute® AutoPak columns deliver fully automated Packing, Repacking, Unpacking and CIP operations which eliminates many of the pain points of packed bed chromatography, resulting in maximum uptime for the customer process. They are available from 400 mm to 2000 mm internal diameter (ID).
- Fully automated “one button” operations ensure repeatable and robust performance
- Multi-axis piston control eliminates the damage to the column from skew
- Complete resin usage
- Air assisted unpacking to save buffer
- “Repack in place” saves expensive media and leads to fast turnaround
Specifications Resolute® AutoPak
Resolute® AutoPak columns are designed for large scale pilot and production use. With a unique combination of active multi-axis piston control, precise linear actuation and patented pack-in-place nozzle, the columns can greatly minimize human errors and deliver a high packing repeatability and minimal downtime. The proven wetted flow path of the columns can provide very low pressure drop and high-resolution chromatography over a wide range of normal operating flow rates (typically from 30 to 800 cm/h).
A typical Resolute® AutoPak is configured with the AutoPak system, 20 μm bed support, an acrylic tube, 3 barg operating pressure and 50 –650 mm packed bed height capability. The columns are available from 400 mm to 2000 mm internal diameter (ID). A range of material options and accessories are available to configure Resolute® AutoPak columns to your requirements.
The Resolute® AutoPak column was developed in direct response to an industry need for fully automated operations. Often key column preparation operations, particularly packing, are regarded as high risk clean room events. These risks include human error, packing failure, and absence of experienced operators; they can result in plant downtime, loss of product and inefficient use of resources.
By adopting a fully automated approach, the Resolute® AutoPak column greatly simplifies operation, effectively eliminates operator error and reduces the burden on key operators. The automated sequences assure that reproducible and consistent operation is the norm, plus the ability to utilize all chromatography resin during packing results in cost savings and less financial variance.
Weights and Dimensions
The table below provides indicative dimensions and weights for the standard range.
For additional diameters and bed heights, please contact Sartorius for an engineered solution.
Resolute® AutoPak Column, Bed Height Range: 50-650 (mm)
Column Size | Length | Depth | Min Height | Max Height | Maximum Height During Maintenance (mm) |
400 | 840 | 1050 | 2000 | 2750 | 2550 |
600 | 1010 | 1060 | 2000 | 2750 | 2510 |
800 | 1360 | 1450 | 2140 | 2890 | 2700 |
1000 | 1510 | 1510 | 2160 | 2910 | 2700 |
1200 | 1800 | 1780 | 2410 | 3160 | 2960 |
Resolute® Linear Drive Station
Weight Empty | Length | Depth | Min Height | Max Height | Maximum Height During Maintenance (mm) |
90 | 510 | 600 | 850 | - | - |
Materials of Construction
The table below lists typical materials of construction. If a material is not compatible with your process, please contact Sartorius for an engineered solution.
Process Wetted Fixed Components | Material | |
Column tube | Acrylic (PMMA) | stainless steel* | |
Distribution cell | Polypropylene | |
Nozzle body | 400-1000 mm ID column: PVDF | 1200 mm ID column: polypropylene (PP) |
Mobile phase termination | 400-1000 mm ID column: PEEK | 1200 mm ID column: stainless steel 316L |
Slurry nozzle trip | PEEK | |
Slurry inlet port | Stainless steel 316L* | |
Main seals | EPDM (peroxide cured) | FEP | |
Nozzle tip seals | FEP encapsulated silicone | |
Wiper ring | PTFE |
All non-wetted materials are suitable for wipe down and clean room use.
Abbreviations: PMMA = Polymethyl methacrylate, PVDF = Polyvinylidene fluoride, PEEK = Polyetheretherketone,
EPDM = Ethylene-propylene-diene monomer (elastomer), FEP = Fluorinated ethylene propylene, PTFE = Polytetrafluoroethylene
* Different grades of stainless steel are available.
Certification and Technical Construction File
Resolute® AutoPak columns are designed to meet CE marking requirements using PD5500 design code. Other certifications and standards can be quoted on request, including ASME, AD Merkblatter, and UL listing.
All columns and accessories come with a full technical documentation file. Examples are available on request.
Additional documentation requirements can be quoted on request. The hard copy of the Resolute® AutoPak software validation documentation is available to review on Sartorius premises if required.
Sartorius has a dedicated network of chromatography experts, who are highly experienced on chromatography process and the column packing.
- Through our Field Applications network, we have the ability to wet demonstrate columns
- Demonstrate column packing in our facilities
- Perform customer specific packing trials
- Recipe development
- Qualification documentation
- Customer training
- Support for the lifetime of the hardware
