Resolute® Linear Columns with AutoPak
Resolute® Linear columns with the AutoPak system deliver fully automated Packing, Repacking, Unpacking and CIP operations which eliminates many of the pain points of packed bed chromatography, resulting in maximum uptime for the customer process. They are available from 350 mm to 2000 mm internal diameter (ID).
- Fully automated “one button” operations ensure repeatable and robust performance
- Multi-axis piston control eliminates the damage to the column from skew
- Complete resin usage
- Air assisted unpacking to save buffer
- “Repack in place” saves expensive media and leads to fast turnaround
Resolute® Linear columns with AutoPak are designed for large scale pilot and production use. With a unique combination of active multi-axis piston control, precise linear actuation and patented pack-in-place nozzle, the columns can greatly minimize human errors and deliver a high packing repeatability and minimal downtime. The proven wetted flow path of the columns can provide very low pressure drop and high-resolution chromatography over a wide range of normal operating flow rates (typically from 30 to 800 cm/h).
A typical Resolute® Linear columns is configured with the AutoPak system, 20 μm bed support, an acrylic tube, 3 barg operating pressure and 50 –550 mm packed bed height capability. The columns are available from 350 mm to 2000 mm internal diameter (ID). A range of material options and accessories are available to configure Resolute® Linear columns with AutoPak to your requirements.
The Resolute® Linear column with AutoPak was developed in direct response to an industry need for fully automated operations. Often key column preparation operations, particularly packing, are regarded as high risk clean room events. These risks include human error, packing failure, and absence of experienced operators; they can result in plant downtime, loss of product and inefficient use of resources.
By adopting a fully automated approach, the Resolute® Linear column with AutoPak greatly simplifies operation, effectively eliminates operator error and reduces the burden on key operators. The automated sequences assure that reproducible and consistent operation is the norm, plus the ability to utilize all chromatography resin during packing results in cost savings and less financial variance.
Weights and Dimensions
The table below provides indicative dimensions and weights for the standard range.
For additional diameters and bed heights, please contact Sartorius for an engineered solution.
Resolute® Linear Column, Bed Height Range: 50-550 (mm)
Maximum Height During Maintenance (mm)
Maximum Height During Maintenance (mm)
Materials of Construction
The table below lists typical materials of construction. If a material is not compatible with your process, please contact Sartorius for an engineered solution.
Process Wetted Fixed Components
Acrylic (PMMA) | stainless steel*
400-1000 mm ID column: PVDF
1200 mm ID column: polypropylene (PP)
Mobile phase termination
400-1000 mm ID column: PEEK
1200 mm ID column: stainless steel 316L
Slurry nozzle trip
Slurry inlet port
Stainless steel 316L*
EPDM (peroxide cured) | FEP
Nozzle tip seals
FEP encapsulated silicone
All non-wetted materials are suitable for wipe down and clean room use.
Abbreviations: PMMA = Polymethyl methacrylate, PVDF = Polyvinylidene fluoride, PEEK = Polyetheretherketone,
EPDM = Ethylene-propylene-diene monomer (elastomer), FEP = Fluorinated ethylene propylene, PTFE = Polytetrafluoroethylene
* Different grades of stainless steel are available.
Certification and Technical Construction File
Resolute® Linear columns are designed to meet CE marking requirements using PD5500 design code. Other certifications and standards can be quoted on request, including ASME, AD Merkblatter, and UL listing.
All columns and accessories come with a full technical documentation file. Examples are available on request.
Additional documentation requirements can be quoted on request. The hard copy of the Resolute® AutoPak software validation documentation is available to review on Sartorius premises if required.
Sartorius has a dedicated network of chromatography experts, who are highly experienced on chromatography process and the column packing.
- Through our Field Applications network, we have the ability to wet demonstrate columns
- Demonstrate column packing in our facilities
- Perform customer specific packing trials
- Recipe development
- Qualification documentation
- Customer training
- Support for the lifetime of the hardware