Chromatography Columns: Designed for Every Molecule and Every Scale
Whether you're developing next-generation biologics, biosimilars, or high-purity small molecules, Sartorius chromatography columns are engineered to deliver reliable performance at any scale.
- Low-pressure chromatography columns provide gentle flow and maintain bed integrity—ideal for large molecules such as mAbs, ADCs, vaccines, and blood or plasma-derived products.
- High-pressure chromatography columns enable high-performance purification of small molecules, with perfectly packed beds and robust flow paths—ideal for insulin, GLP-1, peptides, and oligonucleotides.
Chromatography Columns Portfolio
Smart Chromatography Columns for Every Molecule and Every Process
Whether you're purifying sensitive biologics or small molecules, Sartorius delivers chromatography columns tailored to your resin type, process scale, and regulatory requirements. From fully automated and manual columns for soft bead resins to dynamic axial compression (DAC) columns for hard bead resins, we simplify column packing and streamline performance at every step.
| Prepacked Low-Pressure Chromatography Columns | High-Pressure Chromatography Column
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| Packing | Prepacked | Manual pack-in-place | Manual pack-in-place | Automatic pack-in-place | Dynamic axial compression (DAC) |
| Bed Height | Fixed | Manual adjustment | Automatic adjustment | Automatic adjustment | Automatic adjustment |
| Type of Chromatography Resin | Affinity, ion-Exchange, and mixed-mode | Any low-pressure chromatography resin | Any high-pressure chromatography resin | ||
| Application | Screening and process development | optimization - mAbs, recombinant proteins, and peptides | mAbs, ADCs, vaccines, recombinant proteins, blood | plasma, mRNA, and gene therapy | Insulin, GLP1, oligonucleotides, and peptides | ||
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Supporting Products & Services
Learn More About Chromatography Columns
Decades of Experience. Countless Molecules Purified.
With decades of expertise and engineering leadership, Sartorius has helped shape the evolution of process-scale preparative chromatography. Today, we support the world’s leading biotech, pharma, biosimilar, and CDMO companies. Our chromatography columns are installed in GMP facilities around the globe, powering downstream processes from early-phase development to commercial-scale manufacturing.
- Automated and manual column solutions designed for gentle resin handling and high-resolution performance for large-molecule biologics at production scale
- Dynamic axial compression (DAC): A trusted standard in insulin production, our DAC columns deliver unmatched bed stability and consistency, and are present in virtually every insulin plant worldwide
- Pack-in-place nozzle design: Invented by engineers now part of Sartorius, this innovation revolutionized large-column packing—making high-performance purification faster, safer, and more reliable
- Global support services including process optimization, validation, and training
- Seamless compatibility with leading resins, skids, and automated platforms
Frequently Asked Questions
Sartorius provides a comprehensive range of process-scale chromatography columns, designed for both large molecule (soft bead resins) and small molecule (hard bead resins) applications. Our portfolio includes:
- Fully automated (Resolute® AutoPak) and manual (Resolute® ManuPak or Resolute® Prochrom) columns for gentle handling of sensitive soft bead resins
- Dynamic Axial Compression (DAC) columns for hard bead resins and high-pressure purification processes
- Pilot-scale columns for process development and scale-up
Sartorius columns are compatible with all leading chromatography resins, from soft agarose-based resins used for mAbs, viral vectors, and fusion proteins to rigid polymer- or silica-based resins ideal for small molecules.
Sartorius columns are designed for scalability from pilot-scale models to full production-scale systems. This ensures consistent performance across development, scale-up, and tech transfer stages.
Our experts will work closely with you to recommend the best-fit column based on your specific process needs, including:
- Molecule type (e.g., protein, mAb, ADC, peptide, or small molecule)
- Resin characteristics (soft vs. hard beads)
- Scale of operation
- Regulatory and automation requirements
Both technologies are used in liquid chromatography. High-performance liquid chromatography (HPLC) is typically used for the separation and purification of small molecules. It operates at high pressures, offering high-resolution performance and fast processing times.
Low-pressure liquid chromatography (LPLC) is ideal for purifying large molecules. It operates at lower pressures, allowing for gentler handling of soft bead resins.
Proper maintenance is essential to ensure consistent performance, extended column lifespan, and reliable product quality. Please refer to the user manual for regular spare part replacement and instructions for short- and long-term storage.
