Handbook: 1st Edition Advanced Flow Cytometry

First Edition Advanced Flow Cytometry Handbook —  A Guide to iQue® Advanced Flow Cytometry Assays and Workflows

Flow cytometry is a powerful, laser-based method for analyzing the physical and chemical characteristics of individual cells in a mixed cell population. Yet, non-intuitive workflows, limited throughput, and complicated data analysis schemes of conventional systems, often act as bottlenecks, hindering the progress of countless researchers.

Our easy-to-use digital handbook is designed as a go-to reference for every flow cytometry user. Scientists using traditional cytometers will learn how routine workflows can benefit from the speed, throughput, and superior multiplexing offered by the iQue® Advanced Flow Cytometry Platform. iQue® Platform users can stay up to date on new reagents, assay kits, and software releases, as well as the latest Sartorius publications.

Easily navigate to topics of interest, with informative workflow illustrations, detailed application examples, videos and, direct links to external resources for continued learning. Explore multiplexed analysis of cell phenotype and secreted proteins, in common applications.

  • Document type: Digital eBook / PDF / 19.56 MB
  • Page count: 149
  • Read time: 12hr 00min

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First Edition Advanced Flow Cytometry Handbook: Table of Contents

1. Introduction

2. Advanced Flow Cytometry Cell Health Assays

  • Proliferation and Encoding Dyes
  • Viability Assays
  • Cell Cycle Assessment
  • Apoptosis and Cytotoxicity Assays

3. Advanced Flow Cytometry for Secreted Protein Quantification

4. Advanced Flow Cytometry Assays to Support Cell Line Development

  • Viable Cell Count
  • Titer and Viability Determination
  • Hybridoma Screening

5. Advanced Flow Cytometry Assays for Antibody Discovery

  • Antibody Binding
  • Antibody Internalization (ABI)
  • Antibody-Dependent Cellular Cytotoxicity (ADCC)
  • Antibody-Dependent Cellular Phagocytosis (ADCP)
  • Complement-Dependent Cytotoxicity (CDC)
  • Antibody Cytotoxicity

6. Advanced Flow Cytometry Assays for Immune Cell Profiling

  • T Cell Activation
  • T Cell Killing
  • T Cell Exhaustion
  • T Cell Memory
  • T Cell Killing of Tumor Cells in Advanced 3D Cell Models
  • Tumor Infiltrating Lymphocytes (TILs)
  • Mixed Lymphocyte Reaction (MLR)
  • Natural Killer Cells

7. Advanced Flow Cytometry Assays to Support Cell Therapy

  • Induced Pluripotent Stem Cells (iPSCs) Maintenance and Differentiation
  • Chimeric Antigen Receptor (CAR) T Cells

8. Appendix | Advanced Flow Cytometry Application Notes, Product Guides, and Other Resources

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