Chromatography Resins for High Selectivity, Flexibility, and Productivity
Resin chromatography supports efficient processing by eliminating the need for dilution and adjustment steps, resulting in significant time and cost savings. Designed for scalability, they enable consistent packing and purification from benchtop development through to commercial manufacturing.
Sartorius industry-proven chromatography resins— available as mixed mode, ion exchange, affinity, and hydrophobic interaction—are high-performance resin beads that deliver superior purification and high flow rates.
Packing and purification performance from process development to commercial scale.
Ideal for addressing complex purification challenges while enabling step reduction and greater process efficiency.
Combines hyperdiffusion with ultra-high binding capacities to maximize process performance.
Chromatography Resins Portfolio
Chromatography Resins for Novel and Challenging Modalities
Sartorius chromatography resins enable high-purity recovery in combination with simplified process designs for small and medium-sized biomolecules. They support flexible integration into existing workflows without the need for feedstream adjustment. The broad portfolio addresses diverse process requirements and purification challenges. Unique selectivities offered by mixed mode ligands enhance both capture and polishing steps, increasing the productivity of standard purification platforms.
New Purification Challenges Require a Different Approach
Mixed mode resins combine more than one separation mechanism into one step to achieve higher selectivity and purity. Sartorius offers a broad range of selectivities to address challenges for novel biopharmaceutical targets such as single- and multi-specific mAbs, fusion proteins, high contaminant concentrations, and aggregates, streamlining pre- or post-diafiltration steps and reducing process costs. They are available as bulk resins and pre-packed columns.
Resin | CMM HyperCel | HEA HyperCel | PPA HyperCel | MEP HyperCel | HA Ultrogel® |
|---|---|---|---|---|---|
Modes | Cation exchange, hydrophobic interaction | Anion exchange, hydrophobic interaction | Anion exchange, hydrophobic interaction | Hydrophobic charge induction chromatography (HCIC), pseudo-affinity to IgG (thiophilic) | Cation exchange, Pseudo-affinity |
| Base Material |
| Cellulose | |||
Efficient Molecule Separation for Enhanced Purification
Today’s bioprocessing requires more precision and more efficient operations. Hydrophobic interaction chromatography (HIC) separates molecules based on differences in their surface hydrophobicity. This technology is widely used in chromatographic purification, especially as a more precise purification step from other methods like ion exchange (IEX) or affinity. HIC typically requires a high salt concentration to bind the target protein efficiently. Therefore, it is ideally used after chromatographic steps where elution is accomplished with high salt concentrations, i.e., ion exchange chromatography. Available as bulk resins.
| Resins | Methyl Ceramic HyperD® | SDR HyperD® |
|---|---|---|
| Modes | Hydrophobic interaction and cation exchange chromatography | Hydrophobic interaction and size exclusion chromatography |
| Base Material | Silica | |
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Find the Right Solution for Your Affinity Chromatography Process
Sartorius offers multiple affinity chromatography resins with high binding capacities in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen and growth hormones. Available as bulk resins.
| Resin | Heparin HyperD® | Lysine HyperD® | Blue Trisacryl® |
|---|---|---|---|
| Mode | Affinity Heparin | Affinity Lysine | Affinity Blue dye |
| Base Material | Silica | Silica | Trisacryl |
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A Range of Resins for Efficient and Cost-Effective Process Design
Ion exchange resins are used in the capture and purification of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity in a range of conductivities up to 12 – 15 mS/cm. Unique salt-tolerant matrices, like HyperCel STAR AX anion exchange resin, allow simple process integration without the need for additional feedstream manipulation. Available as bulk resins and pre-packed columns.
| Resin | Q Ceramic HyperD® | HyperCel STAR AX | DEAE Ceramic HyperD® | CM Ceramic HyperD® | SPEC70 SLS |
|---|---|---|---|---|---|
| Mode | Strong anion exchange | Anion exchange | Weak anion exchange | Weak cation exchange | Anion exchange |
| Base Material | Silica | Cellulose | Silica | Silica | Trisacryl |
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Supporting Products & Services
Learn More About Chromatography Resins
Robust Resin Portfolio
Our HyperD® silica platform ensures high productivity through hyperdiffusion and ultra-high binding capacities, whereas our mixed mode selectivities are essential for tackling complex purification challenges, enabling process intensification and step reduction. With industry-proven flexible processing, our resins deliver exceptional packing and purification performance, from process development to commercial scale, making them ideal for efficient and scalable solutions.
Frequently Asked Questions
Chromatography resins are materials used in chromatography to separate and purify molecules. They consist of a solid matrix packed into a column, interacting with sample molecules to separate them based on properties like size, charge, or affinity. Widely used in biopharmaceutical production, research, and diagnostics, they purify proteins, nucleic acids, and other biomolecules.
Chromatography resins are made through chemical and physical processes to create a solid matrix with specific properties. The process includes selecting matrix materials like agarose or silica, cross-linking for stability, functionalizing with chemical groups, forming beads, purifying, and packaging. The specifics vary by resin type and application.
Sartorius chromatography resins are used to purify small- to medium-sized molecules and capture and polish proteins. Mixed-mode resins capture antibodies, enzymes, and proteins. Ion exchange resins, with HyperD® matrix, capture peptides, mAbs, and remove impurities like host cell proteins and DNA. MEP HyperCel resin has a pseudo-affinity for mAbs.
For rigid resins like HyperD®, a compression factor (CF) of 1.05 to 1.1 is ideal. Compressible resins work well with a CF of 1.15 to 1.3. For solutions with varying osmotic properties, a CF closer to 1.3 is recommended. Each resin has an optimal CF, included in Sartorius packing protocols, ensuring progressive compression in hydraulic columns.
A Regulatory Support File (RSF) is available upon request for resin validation, similar to guides for membrane chromatography or filtration. Provided under a Confidential Disclosure Agreement (CDA), Sartorius adheres to current practices by resin suppliers, ensuring compliance and customer support.
