Chromatography Resins

A Robust Portfolio of Resins Offering High Selectivity, Flexibility, and Productivity

Sartorius chromatography resins encompass ion exchange, affinity and mixed mode chemistries coupled with resin beads that offer high flow  and superior purification capabilities. Use of our salt-tolerant or mixed-mode resins can lead to significant time and cost savings by eliminating dilution and adjustment steps. Sartorius resins are industry proven to perform packing and purification from benchtop to commercial scale, with several formats to accommodate  screening through commercial production needs.

Flexible processing

Use of salt-tolerant resins reduce or eliminate the need for dilution or adjustment steps


Unique selectivities

Unique mixed-mode ligands offer powerful separation capability for the most complex purification challenges


Proven scalability

Industry-proven packing and purification performance from process development to commercial scale production


Chromatography Resins for Novel and Challenging Modalities

Sartorius chromatography resins deliver high purity within a simplified process design for small and medium-sized biomolecules. Our resins offer flexible process integration without the need for feedstream manipulation. Our broad portfolio covers our customers’ process requirements and addresses their purification challenges. Our unique selectivity with mixed-mode ligands for both capture and purification will increase the productivity of the existing standard purification platforms.

IEX

Affinity

Mixed-Mode

Ligands 

Q, DEAE, CM, STAR AX 

Heparin, Lysine, Blue Dye 

CMM, HEA, PPA, MEP, HA 

Common applications 

mAbs, recombinant proteins, viruses/VLPs, blood fraction, nucleic acids 

Recombinant proteins, viruses/VLPs, blood fraction 

mAbs, recombinant proteins, fabs, bispecific Abs 

Resin beads 


HyperCel, HyperD® 

HyperD®, Trisacryl® 

HyperCel, Ultrogel® 

Formats 

Bulk resin, PRC pre-packed columns 

Bulk resin 

Bulk resin, pre-packed columns 

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Resin 

Base Matrix

Ligand 

Bead Size 

Binding Capacity 

Q, DEAE, CM HyperD®

Ceramic polymer composite 

Ion exchange 

50 µm 

85 mg/mL* (AEX)
60 mg/mL** (CEX)

HyperCel™ STAR AX 

Rigid cellulose 

Salt-tolerant anion exchange 

80 µm 

100 mg/mL* 

HEA, PPA HyperCel™ 

Rigid cellulose 

Mixed-mode 

80 - 100 µm 

40 – 60* mg/mL 

MEP HyperCel™ 

Rigid cellulose 

Mixed-mode 

80 – 100 µm 

20 mg/mL** 

CMM HyperCel™ 

Rigid cellulose 

Mixed-mode 

50 – 80 µm 

50 – 100** mg/mL 

HA Ultrogel® 

HA agarose composite 

Mixed-mode (hydroxy-apatite) 

60 – 180 µm 

7 mg cytochrome C /ml

Blue Trisacryl®

Trisacryl  

 Affinity blue dye 

40 – 80 µm 

10 mg/mL* 

Heparin HyperD®

 Polymer composite 

Affinity heparin

80 µm 

25 mg ATII/mL 

Lysine HyperD® 

 Polymer composite 

Affinity lysine

70 µm 

* Binding capacity is related to BSA
** Binding capacity is related to IgG

IEX Resins

A Range of Resins for Efficient and Cost-Effective Process Design

Ion exchange resins are used in the capture and purification chromatography steps of various biomolecules. Sartorius offers a comprehensive range of chemistries and bead matrices. The resins provide high capacity at short residence times in a flexible range of conductivities up to 12 – 15 mS/cm. Unique salt-tolerant matrices, like HyperCel Star AX anion exchange resin, allow simple process integration without the need for additional feedstream manipulation. 

Q Ceramic HyperD®

HyperCel STAR AX

DEAE Ceramic HyperD®

CM Ceramic HyperD®

Ligand

Quaternary amine

Primary amine

Diethylaminoethyl

Carboxymethyl

IEX type

Strong anion exchange

Weak salt-tolerant anion exchange

Weak anion exchange

Weak cation exchange

Working conductivity

4-8 mS/cm

Salt tolerant: 2-15 mS/cm

4-8 mS/cm

Salt tolerant: up to 10-19 mS/cm

Bead material


Ceramic polymer composite (50 µm)

Rigid cellulose
(80 µm)

Ceramic polymer composite (50 µm)

Ceramic polymer composite (50 µm)

DBC at 10% breakthrough

BSA ≥85 mg/mL

BSA ≥100 mg/mL

BSA ≥85 mg/mL


IgG ≥60 mg/mL

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Q Ceramic HyperD®

Q Ceramic HyperD® is a strong anion exchanger that is suited for the removal of anionic impurities (i.e., host cell protein and DNA). Its 50 µm base bead offers a high resolution for purification applications when required.

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DEAE Ceramic HyperD®

DEAE Ceramic HyperD® is a weak anion exchanger for purification. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.

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CM Ceramic HyperD®

CM Ceramic HyperD® is a weak cation exchanger for polishing applications. Changes in conductivity and pH can optimize purification steps by utilizing the charge change on protein and ligand.  

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HyperCel STAR AX

HyperCel Star AX is a salt-tolerant anion exchanger with a primary amine as functional group. It can easily be integrated into a purification workflow without feedstream manipulation.

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High-Capacity Affinity Resins

Find the Right Solution for Your Affinity Chromatography Process

Sartorius offers multiple affinity chromatography resins with high binding capacities in a variety of selectivities to purify recombinant proteins, albumin, enzymes coagulation factors (ATIII), plasminogen and growth hormones.

Heparin HyperD®

Lysine HyperD®

Blue Trisacryl® M

Applications

Coagulation factors (ATIII), growth hormones and enzyme purification

Plasminogen, recombinant protein and glycoprotein purification

Albumin, enzymes, recombinant proteins and purification

Dynamic binding capacity (10 % BT)

> 25 mg/mL

-

> 10 mg/ml

Average particle size

80 µm

70 µm

60 µm

Working pH range

3 –13

3 –13

1 – 10

Operating pressure

<3 barg (44 psig)

<3 barg (44 psig)

-

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Heparin HyperD®

Heparin HyperD® M resin is suited for the purification of heparin-binding proteins like coagulation factors, growth hormones, lipoproteins, and DNA/RNA processing enzymes.

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Lysine HyperD®

Lysine HyperD® affinity resin is used for the purification of lysine-binding proteins such as plasminogen. It provides high protein-binding capacities at high flow rates.

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Blue Trisacryl® M

Blue Trisacryl® M is an affinity resin used for the purification of enzymes, interferons, and some coagulation factors as well as for the removal of albumin. The resin uses Trisacryl® based beads which carry the blue dye ligand.

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Mixed-Mode Resins

New Purification Challenges Require a Different Approach

Novel biopharmaceutical targets such as nanobodies, plural-specific mAbs, and fusion proteins require unique selectivities to achieve high product purity. Mixed-mode resins leverage multi-modal separation mechanisms such as IEX and HIC to achieve higher selectivity and purity in a single step than in traditional single-mode chromatography.

Sartorius offers a broad range of selectivities to address challenges with a high concentration of contaminants with the intensified feedstreams, aggregates issues with newer modalities, and to remove challenging process or product-related impurities. Achieve reduced process costs by streamlining pre- or post-diafiltration steps.

CMM HyperCel

MEP HyperCel

HEA HyperCel

PPA HyperCel

HA Ultrogel®

Modes

CEX

HIC

Hydrophobic charge
induction (HCIC)
pseudo-affinity
to IgG (thiophilic)

AEX

HIC

AEX

HIC

CEX

Pseudo-affinity

Application

B/E antibody
HCP, aggregate removal
recombinant molecule purification

Capture of antibodies in physiological conditions (pseudo affinity) only for MEP HyperCel
HCP, aggregate, variants, removal
antibody, fab, enzymes, recombinant proteins

 

Dynamic binding capacity

IgG
>60-100 mg/mL*

Human IgG
>20 mg/mL*

BSA
40-60 mg/mL*

BSA
40-60 mg/mL*

Cytochrome C
>7 mg/mL**

Average particle size (µm)

50-80

80-100

80-100

80-100

60-180

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* 10% BT
** 50% BT

CMM HyperCel

CMM HyperCel is a hydrophobic cation exchanger recommended for the capture of antibodies, antibody fragments, and recombinant proteins. It allows the separation of proteins with similar pI by modulation of pH and conductivity.

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MEP HyperCel

MEP HyperCel provides unique selectivity for monoclonal antibody capture or purification to remove aggregates, HCP, and DNA. The ligand facilitates the binding of target proteins at neutral pH under moderate salt concentrations.

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HEA HyperCel

HEA HyperCel is a mixed-mode ligand with a less hydrophobic hexylamine chain allowing protein binding at a moderate salt concentration under neutral pH.

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PPA HyperCel

PPA HyperCel exhibits a stronger hydrophobicity with an aromatic ligand, allowing protein binding at a moderate salt concentration under neutral pH.

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HA Ultrogel®

HA Ultrogel® hydroxyapatite is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed-mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. It is ideally suitable for general impurity removal.

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