Pre-Packed Chromatography Columns

For High Throughput Chromatography Sorbent Screening and Optimization

ScreenExpert RoboColumns are miniaturized chromatography columns pre-packed with chromatography sorbents. They are designed for fully automated and parallel chromatographic separations with robotic liquid handling workstations, such as the Freedom EVO^® from Tecan.

ScreenExpert RoboColumns are packed with anion exchange, mixed-mode,affinity or any other chromatography sorbents upon request. 

Two formats are available, 200 μL and 600 μL, with a bed height of 10 and 30 mm respectively. The 200 μL column volume is recommended for sorbent and process conditions screening, while the 600 μL is used for purification process optimization or when a residence time above 4 minutes is required (e.g., capacity study on mixed-mode sorbents).

The columns are supplied as rows of eight pre-packed units, with two removable silicon cover seals for proper storage. A 96-well array plate is available to arrange up to 96 ScreenExpert RoboColumn units.

Benefits

• Column format, and high quality packing enables the ability to mimic all the steps of a chromatography separation in dynamic mode. Data obtained are comparable to those obtained in larger columns
• Miniaturized format facilitates screening of chromatography sorbents, reduces sample consumption, and saves time
• Perfectly fitted to Design of Experiment (DoE) requirements, allowing a large amount of experimental work in a minimum of time
• 96-well format allows easy integration with automation systems for High Throughput Process Development (HTPD)
• Multiple parameters can be tested in parallel, e.g., residence time
• Reusable
• Flexible use: the user makes column selection when needed

Handling

ScreenExpert RoboColumns are designed for robotic handling.

When using the high-throughput process development approach for ScreenExpert RoboColumns, it is suggested to use Design of Experiments (DoE) criteria for the experimental set-up. This enables to screen many different chromatographic parameters such as resin type, pH, conductivity / ionic strength, etc., efficiently and simultaneously.

For experimental set-up and the configuration of the 96-well array plate, the Te-Chrom Wizard is recommended. The Te-Chrom Wizard is a dialog-based graphic user interface offered by Tecan and based on the Freedom EVOware software. The Te-Chrom Wizard was developed for the use of ScreenExpert RoboColumns and provides the possibility to configure hardware, plate layout, and the chromatographic process as well as to set all process relevant chromatographic parameters, e.g., flow rate, volume without direct script writing.
 

* MediaScout and RoboColumn are trademarks of Atoll GmbH. LabChip is a trademark of PerkinElmer. Freedom EVO, Freedom EVOware, and Te-Chrom are trademarks of Tecan Group.

ScreenExpert RoboColumns are suitable for a large variety of applications such as:

• Parallel screening of chromatography sorbents
• Parallel screening and optimization of chromatographic conditions in downstream process development (conductivity, pH)
• Scale down experimental work
• Determination of dynamic binding capacity
• Determination of the optimal residence time
• Optimization of the regeneration step

¹ The Liquid Transfer menu of the Te-Chrom™ Wizard enables set up of the flow rate for each individual dispensing and chromatography step (5.1.1.6 Liquid Transfer, Te-Chrom Wizard Software Manual, 396076, en, V1.0).

Multi-Well Plates (empty)

AcroPrep Advance 96-Well Filter Plate for Ultrafiltration

PRC Prepacked Columns

Rapid selectivity screening of HyperCel STAR AX anion exchanger.

• Convenient – Ready-to-use 1 mL and 5 mL prepacked columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems.
• Efficient – High packing efficiency (≥ 2,500 plates/meter).
• Consistent – Screen HyperCel STAR AX sorbent under reliable and reproducible conditions, and guarantee performance run after run.

"Salt-Tolerant" Anion Exchange Chromatography Sorbent

HyperCel STAR AX sorbent is composed of a rigid cellulose matrix that has excellent flow properties and generates low backpressure. It provides:

• High dynamic binding capacity at short residence time
• Use in either capture, intermediate or polishing steps
• High dynamic binding capacity is maintained up to 15 mS/cm conductivity
• Direct scale up to pilot or production scale columns

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate separation conditions. Once the separation conditions are selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications. Further scale up can be achieved with minimal re-optimization by packing Pall LRC empty glass columns.

HyperCel STAR AX Anion Exchange Chromatography Sorbents

• Native and recombinant proteins
• Vaccines
• Monoclonal and polyclonal antibodies
• Plasma derivatives
• Biopharmaceuticals

¹ Determined at 10% breakthrough, using 5 mg/mL bovine serum albumin (BSA) in 50 mM Tris-HCl, pH 8.5 at 2 minute residence time (RT). Conductivity 2 – 15 mS/cm by supplementation of NaCl.

² Pressure at 600 cm/hr equivalent to: 2 mL/min for 1 mL column, 5 mL/min for 5 mL column, in 0.1 M NaCl.

Rapid selectivity screening of MEP, HEA, and PPA HyperCel mixed-mode sorbents

• Convenient – Ready-to-use 1 mL and 5 mL prepacked columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems.
• Efficient – High packing efficiency (> 2,500 plates/meter).
• Consistent – Screen mixed-mode chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

Mixed-mode Chromatography Sorbents for Antibody Capture and “No Salt” Hydrophobic Interaction

HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants. They provide a unique separation mechanism different from conventional methods. All ligands operate predominantly by hydrophobic interaction. This “HIC-like” interaction typically takes place without the addition of lyotropic salt.

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry. Once the chemistry is selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column in doubling the height.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications.

Mixed-mode Chromatography Sorbents for Antibody Capture and “No Salt” Hydrophobic Interaction

• MEP HyperCel sorbent: Antibody capture; alternative to conventional hydrophobic interaction
• MEP, HEA, PPA HyperCel sorbents: No salt/low salt alternative to hydrophobic interaction

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)
5 mL (8 mm ID x 100 mm)

Functionalities
Mixed-mode: MEP, HEA and PPA HyperCel sorbents

Storage Solution
30% isopropanol/100 mM sodium phosphate, pH 4.3

Working Pressure¹
< 0.5 barg (7 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns
11.5 x 140 mm for 5 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns
30 barg (435 psig) for 5 mL columns

¹ Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.

¹ Determined at 10% breakthrough using:
² 5 mg/mL human IgG in PBS, flow rate 60 cm/h
³ 5 mg/mL BSA in PBS, flow rate 100 cm/h

Sartorius offers a comprehensive line of chromatography columns from lab to process scale.

PRC Prepacked Sorbent Ordering Information

Rapid selectivity screening of Ceramic HyperD ion exchangers

• Convenient – Ready-to-use 1 mL prepacked columns.
• Easy to use – Direct connection to commonly-used laboratory chromatography systems.
• Efficient – High packing efficiency (> 2,500 plates/meter).
• Consistent – Screen Pall ion exchange chromatography sorbents under reliable and reproducible conditions, and guarantee performance run after run.

Ion Exchange Chromatography Sorbents

Q and CM Ceramic HyperD sorbents have a good capacity hydrogel polymerized within the gigapores of a rigid ceramic bead. Typical features include:

• Good dynamic binding capacity independent of flow rate
• Allowing a direct capture with high binding capacity for IgG at conductivities of 10 to 15 mS/cm on CM Ceramic HyperD sorbent

PRC columns help to determine the best selectivity and resolution in optimal conditions of packing and use.

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications.

Ion Exchange Chromatography Sorbents

• Native and recombinant proteins
• Plasmids
• Vaccines
• Monoclonal and polyclonal antibodies
• Plasma derivatives
• Biopharmaceuticals

Sorbent Specifications

Volume
1 mL (5 mm ID x 50 mm)

Functionalities
Ion exchange: Q and CM Ceramic HyperD F sorbents

Storage Solution
20% ethanol/150 mM NaCl

Working Pressure¹
< 1.5 barg (22 psig)

Column Specifications

Outer Dimensions
10 x 100 mm for 1 mL columns

Materials of Construction
Body and end caps: Molded polypropylene
17 µm frit: PP/PE (Polypropylene/Polyethylene)

Connections
Built-in 10-32 fittings

Maximum Operating Pressure
20 barg (290 psig) for 1 mL columns

¹ Pressure at 600 cm/h equivalent to 2 mL/min in 0.1 M NaCl.
 

¹ Determined at 10% breakthrough using:
² 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6, flow rate 200 cm/h
³ 5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7, flow rate 200 cm/h