Resolute® BioSMB Platform

Single-Use Multi-Column Chromatography from Process Development to Manufacturing

Process intensification is an optimal solution to address chromatography challenges, providing substantial improvements to productivity, cost, timelines, and facility footprint. Our flexible portfolio lets you choose an intensification strategy that aligns with your unique process goals.

Multi-column chromatography supports better resin utilization, meaning more product is bound in less time and at a reduced cost. The Resolute® BioSMB is the perfect solution for those seeking lower production costs and limit resin usage with minimal disruption to current chromatography methods.

Up to 80% reduction in resin volumes and costs

Improved resin utilization and reduced resin quantities, decreasing overall manufacturing costs

Reduced buffer consumption

Up to 2x reduction in buffer volumes, improving sustainability

Scalable single-use platform

Uses the same valve technology, recipes, and flowpath configuration for process development and manufacturing systems, streamlining scale-up

Resolute® BioSMB Platform for Both Batch and Continuous Processes

Resolute® BioSMB platform is a family of chromatography systems designed to purify biologics from process development to commercial manufacturing scale. Resolute® BioSMB is versatile and can be deployed as part of a traditional batch process or a continuous downstream process.

Resolute® BioSMB  PDResolute® BioSMB 80

Resolute® BioSMB 350


Process Development

Clinical | Commercial Manufacturing

Clinical | Commercial Manufacturing

Number of Column Positions

1 to 16

1 to 8

1 to 8

Flow Rate Range

1 mm cassette: up to 50 mL/min
3 mm cassette: up to 100 mL/min

5-80 L/hr (83-1300 mL/min)

30-350 L/hr (500– 5800 mL/min)


Process development

Typically for perfusion-based or smaller processes up to 500 L bioreactor

Typically for larger processes up to 2000 L bioreactor

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Request A Demo

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Request A Demo

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Enhanced Process and Quality Control for MCC Using UPLC Technologies

Learn how we integrated the Waters™️ PATROL UPLC Process Analysis System (an at-line PAT tool) into the Resolute®️ BioSMB to optimize continuous downstream operations.

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Learn More About Resolute® BioSMB Systems

Virtual Demo of the Resolute® BioSMB PD System

To learn about the ease of installation, set-up, and main features of the Resolute® BioSMB PD system, watch this video.

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Virtual Demo of the Resolute® BioSMB 80 | 350 System

To learn how the Resolute® BioSMB platform can be easily scaled up from early-stage process development through clinical manufacture and beyond, watch this video.

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Simplify the Management and Analysis of Resolute® BioSMB PD Data

With our Resolute® BioSMB Data Analysis and Reporting software, users can speed up processing large volumes of Resolute® BioSMB PD data utilizing a user-friendly interface. This software is designed to:

  • Perform a wide range of chromatography calculations on both peaks and transitions
  • View and analyze historical Resolute® BioSMB PD results rapidly
  • Visualize Resolute® BioSMB PD data from multiple cycles or runs

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Traditional Batch vs Multi-Column Chromatography

Continuous Multi-Column Chromatography: Better Than Batch Chromatography

In traditional single-column batch chromatography, there are inherent limitations because the system rarely uses the full capacity of the stationary phase during loading. In multi-column chromatography, the large column is split into a number of smaller columns that can be processed individually, eliminating idle zones. In addition, the flexible valve configuration allows breakthrough of the product stream to a secondary column, which allows the full capacity of the column to be used through overloading, leading to improved process efficiency with increased resin capacity utilization. 

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How to Debottleneck Downstream using Multi-Column Chromatography

Making the switch from single batch to multi-column chromatography can dramatically reduce resin volumes, reducing costs and eliminating bottlenecks without sacrificing quality. This animated feature compares the traditional, single column batch approach to a multi-column alternative for mAb capture.


The Medicine Maker Webinar: Downstream Process Intensification

Join Sartorius as we explore solutions for downstream process intensification. Our experts will discuss key technologies such as chromatography.

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Achieve 3-5x Higher Productivity in 5 Days or Less

The switch from batch to multi-column chromatography has long been a costly and time-consuming process. The BioSMB platform changes the game.

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Increase Throughput 6X with Multi-Column Chromatography

Explore how Resolute® BioSMB multi-column chromatography overcomes the limitations of batch chromatography and leads to sixfold gains in throughput.

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Featured Assets

Case Study

Protein A Resin Reduction Achieved with Transfer to Resolute® BioSMB

Read how Sanofi went from a batch to a MCC process, scaled up to continuous perfusion, and gained productivity while cutting resin use.

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Success Story

Resolute® BioSMB Increased Productivity at mAB Capture Step

A CDMO transformed their production capabilities after adopting the BioSMB process. Read how it lowered costs and increased productivity.

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White Paper

Moving to Multi-Column Chromatography: A Beginner’s Guide

This white paper introduces multi-column chromatography, describes its advantages over batch chromatography, and provides a step-by-step guide to tran...

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Articles,  Application Notes & White Papers

White Paper

Re-Imagining Chromatography - Building Efficient, Connected Downstream Processes

PDF | 2.4 MB
Case Study

Merck Process Transfer and Scale-Up of Batch Protein A Chromatography to Resolute® BioSMB Multi-Column Platform

PDF | 577.3 KB

(Re)Defining Process Intensification

PDF | 343.7 KB
Application Note

Resolute® BioSMB 80/350 System - Scale-up of Multi-Column Chromatography

PDF | 469.8 KB

Resolute® In with the New: Embracing Multicolumn Chromatography

PDF | 595.7 KB



Multi-Column-Chromatography Process Modelling for Process Performance Prediction

PDF | 233.8 KB

Simplify Adoption: Your First Week of Resolute® BioSMB

PDF | 522.4 KB



Resolute® BioSMB PD System - Continuous Single-Use, Multicolumn Chromatography

PDF | 408.3 KB

Resolute® BioSMB Data Analysis Software

PDF | 302.0 KB

Resolute® BioSMB 80 & 350 System

PDF | 1.0 MB

Consult Our Experts

Consult Our Experts

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