Continuous Chromatography

Multi-Column Chromatography Solutions for Minimizing Costs

The Sartorius chromatography systems portfolio gives the ability to intensify a process through two pathways. At the unit operation level, the BioSMB leverages Mult-Column Chromatography (MCC) to intensify any chromatography unit operation through a decrease in media usage or processing time and the ability to expand the performance envelope chromo step outside of that delivered by the batch equivalent. This could be as part of a batch or continuous process.
The other path through the Resolute® BioSC technology (Multi-Step Chromatography) allows the intensification at process level, where multiple unit operations are joined together – saving time and footprint through the consolidation of unit operations within one integrated system and the ability to conduct parallel unit operations. The Resolute® BioSC also provides a route for multi-use multi-column operations.

Choose the Right System

Find the Right Multi-Column Chromatography Solution

The BioSMB platform is a fully scalable solution for your manufacturing environment and is supported by a global Field Application Services team with MCC experts who can provide installation support. BioSMB PD is designed for process development and small-scale manufacturing, while BioSMB Process systems are designed for clinical and commercial manufacturing. In the latter, there are two flow range variants available — BioSMB Process 80 and BioSMB Process 350. The two systems cover typical single-use perfusion and fed-batch bioreactor-based processes.


BioSMB PD

BioSMB Process 80 | 350

Resolute® BioSC

Scale

PD / Clinical

Clinical | Commercial Manufacturing

Clinical | Commercial Manufacturing

Intensification LevelUnit operationUnit operationUnit operation | Integrated process

Core Capability

Multi-column chromatography

Multi-column chromatography

Multi-step chromatography

Mode

Single-use

Single-use

Multi-use

Explore BioSMB PD

Explore BioSMB Process

Explore Resolute® BioSC


BioSMB PD

BioSMB PD is an integrated single-use device for process development to fully automate a wide variety of chromatographic processes, including bind/elute chromatography and flow-through polishing.

The BioSMB PD system is designed specifically for lab-scale process development and features:

  • Up to 16 column positions
  • Flow rate range of .06–6 L/hr
  • 2 sizes of disposable flow kit, changed as part of preventive maintenance

Explore BioSMB PD     Request a Demo

BioSMB Process 80/350

The BioSMB Process 80 and 350 systems are sized and specified for clinical and commercial manufacturing under GMP. They feature a unique single-use, eight-column architecture for a highly flexible chromatography system that delivers high throughput and very efficient resin utilization.

There are two flow range variants available — BioSMB Process 80 and BioSMB Process 350. The two systems cover typical single-use perfusion and fed-batch bioreactor-based processes:

  • Up to 8 column positions
  • Flexible flow configuration through single-use valve cassette
  • Flow rates ranging from 5-350 L/hr
  • Interchangeable pump modules that can be swapped between systems

Explore BioSMB Process     Request a Demo

Resolute® BioSC

Resolute® BioSC low pressure liquid chromatography (LPLC) platform enabling batch, multiple multi-column processes (parallel batch, continuous chromatography) or multi-step integration resulting in Process Intensification. 

Explore Resolute® BioSC

Virtual Demo of the BioSMB MCC Platform

To learn more about how the BioSMB platform reduces resin utilization by 80% versus batch chromatography and enables rapid process scale-up, watch the...

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Traditional Batch vs. Multi-Column Chromatography

Continuous Multi-Column Chromatography: Better Than Batch Chromatography

In traditional single-column batch chromatography, there are inherent limitations because the system rarely uses the full capacity of the stationary phase during loading. In multi-column chromatography, the large column is split into a number of smaller columns that can be processed individually, eliminating idle zones. In addition, the flexible valve configuration allows breakthrough of the product stream to a secondary column, which allows the full capacity of the column to be used through overloading, leading to improved process efficiency with increased resin capacity utilization. 

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Batch Chromatography

  • Single large column
  • Column capacity limited to prevent product loss
  • Cycle times influenced by high operational residence times and low linear flow rates
  • Resin requirements heavily influenced by batch sizes and processing time (desired throughput)
  • Large columns require significant clean room space and support infastructure

Multi-Column Chromatography

  • Number of smaller columns  
  • Efficiency Gain 1: Column overloading reduces risk of product loss and increases ability to operate at higher binding capacities
  • Efficiency Gain 2: Continuous cycling operation eliminates non-loading idle time
  • Resin volume requirements reduced by up to 80% without impacting process throughput
  • Small footprint, eliminating the need for packing skids and other support infrastructure

How to Select Your DSP Chromatography Technology

Making the switch from single batch to multi-column chromatography can dramatically reduce resin utilization, reducing costs and eliminating bottlenecks without sacrificing quality. This animated feature compares the traditional, single column batch approach to a multi-column alternative for mAb capture.

Learn More About Continuous Chromatography

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Featured Assets

Success Story - BioSMB for Increased Productivity at mAB Capture Step

Our experts recommended that a CDMO upgrade from traditional batch chromatography to the BioSMB multi-column chromatography platform to achieve higher productivity at the mAb capture step. Read how the CDMO saw dramatic improvements in both resin utilization and measured productivity.

Read the Success Story

Case Study - Merck Process Transfer and Scale-Up of Batch Chromatography to Multi-Column Platform

The driver for the study was an exploratory evaluation of the impact and benefits of multi-column chromatography. Product quality consistency was considered, as well as the possible cost and resin savings associated with transitioning from batch to continuous.

Read the Case Study

Related Assets

Poster

MCC Process Modelling for Process Performance Prediction

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Infographic

5 Reasons to Switch to Multi-Column Chromatography

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Article

In with the New: Embracing Multicolumn Chromatography

PDF 595.7 KB

Consult Our Experts

Consult Our Experts