Enabling Cell Line Selection, Process Characterization and Process Optimization in a Quick and Efficient Way
Comparing cultures in identically-sized, multi-parallel bioreactors allow scientists to find out more process information in a shorter timeline. Multiple experiments can be set up to evaluate different culture strains and the effect of process parameters, such as temperature, feeding, media composition, gassing rates and inoculation densities.
The industry-leading range of Ambr® multi-parallel bioreactors has been developed to take you through the early steps of your upstream process from cell or strain selection, through to process optimization. The technology ensures optimum scalability of your processes, as the design of the bioreactors means that functionality translates well into systems such as the Biostat STR® 2000 and larger.
Find Your Multi-Parallel Bioreactor
A range of analyzer technologies compatible with our Ambr® 15 and Ambr® 250 systems
Ambr® systems are compatible with a variety of integrated and data-connected analyzers to support a comprehensive range of bioprocessing needs.
Follow the link below to find out which analyzers are compatible with each Ambr® system and learn about the key features and benefits.
High Throughput Bioprocess Development
Kistler, Pollard, Lee, Streefland (2016) Genetic Engineering & Biotechnology News (Vol. 36, No. 7) Apr 2016
Bioprocess engineers are achieving better process understanding and more optimal cell culture conditions by using high throughput scale-down methods and design of experiment (DOE) approaches.
This bioprocessing tutorial explains how process development scientists can use the Ambr® 250 automated bioreactor system to screen a wide process design space while minimizing resources and materials.
FAQs - Ambr® | Multi-Parallel Bioreactors
Multi-parallel bioreactors allow multiple comparable experiments to take place at low volumes, at the same time. This is important for many areas of upstream research including clone selection, feed / media optimization, process characterization, process intensification, advanced therapies and quality, consistency / reproducibility.
Ambr® 15 reduces the cost per experiment by saving substantial amounts on facility space, capital, labor, media and consumables. Ambr® 15 helps teams to select better clones, screen more media and optimize processes, faster.
Ambr® 250 systems reduce the cost per experiment by substantial savings on facility space, capital, labour, media and consumables. The systems provide a high precision environment that supports the demands of microbial fermentation and mammalian cultures.
The Ambr® 250 High Throughput is a fully automated platform on which scientists can perform efficient and rapid scale-down studies to explore a wide range of conditions and strains with scalability to both bench top and larger stirred tank bioreactors.
Ambr® 250 Modular is not fully automated: it uses the same advanced stirred tank bioreactor technology as Ambr® 250 High Throughput, but with manual filling and sampling. Each vessel has integrated sensors, liquid reservoirs and syringe pumps which make it possible for experiments to be set up and turned around rapidly.
Each bioreactor vessel can run a different culture or different conditions if required:
An Ambr® 15 Cell Culture system has capacity to run 24 or 48 microbioreactor vessels simultaneously.
An Ambr® 250 system has capacity to run 12 or 24 bioreactor vessels simultaneously.
An Ambr® 250 Modular system has capacity to run 2 bioreactor vessels per module. Starting with 1 module, the system can be extended to run up to 4 modules (8 bioreactor vessels).
Small scale multi-parallel bioreactors can be used for early studies, process optimization and characterization of intensified culture conditions and processes.
When the process has been fully characterized at small scale the intensified manufacture can take place. Early studies can be done in Ambr® 15 in perfusion-mimic mode, with process optimization and characterization and optimization in Ambr® 250 High Throughput Perfusion. The smart single-use bioreactor also includes an industry standard hollow fibre membrane for the best prediction of large scale process performance.
This highly scalable but small volume system allows users to predict large scale perfusion performance, while dramatically reducing the very high media costs of perfusion bioreactor studies. With a compact design, users can fit many more perfusion bioreactors into the laboratory, increasing capacity for perfusion experiments without complex infrastructure projects. Fully integrated irradiated consumables save time, reduce staff costs and free up scientists to focus on the science. The finalized intensified process can be run in a large bioreactor such as Biostat STR®.
Using multi-parallel bioreactors to design a process suitable for scale up or for scale down model verification can help reduce project timelines and resources, as many more experiments can be carried out at the same time and at a smaller (and less expensive to run) scale.
To further enhance the scalability of Sartorius bioreactors, BioPAT® Process Insights software can be used to simplify process transfer and predict the scaling risk as you scale up or down.