Live-Cell Imaging & Analysis Software
Incucyte® software makes the process of acquiring, viewing, analyzing and sharing images of living cells easier than ever before. Enabling researcher teams to convert images into insight with the base software’s purpose-built analysis tools to comprehensive software modules that enable powerful phenotypic cellular analysis.
Incucyte® Base Analysis Software
The Incucyte® Base Analysis Software simplifies the complex yet provides a powerful workflow that supports your entire research team.
A guided interface and purpose-built tools make the process of acquiring, viewing, analyzing and sharing images of living cells easier than ever before using the Classic or new AI-driven Analysis.
Stress-free Image Acquisition
- Guided interface enables even first-time users to set up long-term, live-cell experiments
- Flexible scheduling tool allows multiple users to run multiple applications in parallel
Insight-generating Visualization
- Incucyte® Vesselview displays a birds-eye view of the entire vessel to give you a snapshot of your experiment, plus tools to quickly navigate through images and time
- Quickly assess trends and outliers, and validate with metric overlays
Powerful, Purpose-built AI-driven Analysis
- Guided interface makes processing thousands of wells of data easier, driven by AI or Classic segmentation
- Graphing tools designed by biologists enable publication-ready graphs without the need for third-party software
Effortless Data Management with Auto Archive
- Automatically archive and manage your data with Incucyte® Auto Archive
- Included free with S3 and SX5 systems for seamless, stress-free data storage
Incucyte® Base Software Workflow
Incucyte® AI Confluence Analysis Workflow
AI-driven Confluence Analysis provides a simple workflow for highly accurate segmentation of cells in phase contrast images, adapting to a wide range of cell types and morphologies with minimal user input. AI Confluence Analysis is available with Incucyte® Software v2022A or newer software version.
Incucyte® Classic Confluence Analysis Workflow
The Classic Confluence Analysis enables you to tailor phase segmentation parameters to individual cell types and experimental conditions to accurately distinguish between background and positive fluorescent objects across a wide range of intensities, and depending on the optical module, allows for the quantification of green, orange, red, and/or NIR fluorescent objects within each image.
Incucyte® Base Software Overview
Acquire
Incucyte® Acquisition interface guides users through experimental set up using your choice of microplates, flasks and dishes. Simply select parameters and hit “Start” for fool-proof, continuous live-cell image acquisition.
View
Incucyte® VesselView enables users to navigate and explore thousands of timelapse images with unprecedented efficiency. By viewing images of all locations in your experiment at once, you can quickly assess treatment effects or identify outliers. You can also overlay metrics for rapid assessment and verification of image processing parameters. Then, generate presentation-ready images and movies with just a few clicks.
Easily view images of all locations in an experiment at once to scan for trends and outliers, then zoom in on wells of interest.
Analyze
Incucyte® Analysis interface enables even first-time users to convert images into insights. By providing purpose-built tools to answer your scientific questions, image processing and analysis is uncomplicated yet extremely powerful. Create analysis definitions once, then reapply to subsequent experiments to generate real-time metrics that enable decision-making. Easily view all trends in an experiment at once with Incucyte® Microplate Graphs, and create customized response curves that are ready for publication and presentation
Guided analysis interface enables even first-time users to convert images into insights using powerful AI-driven or classic segmentation for confluence measurements.
Graphing tools enable review of trends and rapid generation of publication- and presentation-ready graphs.
Incucyte® Featured Software Modules
Featured Software Modules
Incucyte® Additional Software Modules
Additional Software Modules
Incucyte® System Software Updates
| Incucyte® Software (v2025B) | The latest Incucyte® software update (v2025B) is now available, introducing powerful new capabilities to advance your image-based research. | Explore More |
| Incucyte® Software (v2025A) | The Incucyte® software release (v2025A) delivers powerful new functionality to make data management easier and more secure than ever. | Explore More |
| Incucyte® Software (v2024B) | Incucyte® software update (v2024B) is available for Incucyte® Live-Cell Analysis Systems, providing new features that have been highly requested. | Explore More |
| Incucyte® Software (v2020C) | The new Incucyte® software update (v2020C) is available for Incucyte® Live-Cell Analysis Systems, providing new and highly requested security and image processing features. | Explore More |
| Incucyte® Software (v2019B) | The Incucyte® Software update (v2019B) is available for Incucyte® Live-Cell Analysis Systems, providing highly requested features. | Explore More |
| Incucyte® Software (v2019A) | The Incucyte® Software update (V2019A) is available for the Incucyte® S3 Live-Cell Analysis System to provide you with new features that have been highly requested by our users plus the ability to classify adherent cell populations with our Cell-by-Cell Analysis Software Module. | Explore More |
| Incucyte® S3 Software (v2018B) | The Incucyte® S3 Software update (V2018B) is available for the Incucyte® S3 Live Cell Analysis System, providing metric normalization, new processing definitions from existing, and archive analysis. | Explore More |
| Incucyte® S3 Software (v2018A) | The Incucyte® S3 Software update (V2018A) is available for the Incucyte® S3 Live-Cell Analysis System to provide you with new features that have been highly requested by our users plus more versatility when using the Spheroid Software Module to acquire and analyze images of three-dimensional tumor spheroids. | Explore More |
