Incucyte^® Advanced Label-Free Classification Analysis Software Module  (Cat. No. BA-04867)

The Incucyte^® Advanced Label-Free Classification Analysis add-on for the Incucyte^® Cell-by-Cell Analysis or AI cell Health Analysis enables automated identification of cell morphology changes using label-free, integrated image acquisition, segmentation and multi-variate analysis of cell shape. Track biological changes using unbiased, smart defaults – opening up new possibilities in comparison of test results across experiments to:

• Classify cells automatically and label-free based on morphology
• Perform unbiased analysis of cell morphology
• Conduct label-free phenotypic screening
• Label-free analysis of morphology changes within live or dead cell subpopulations

Capturing data-rich information without the use of fluorescent labels has never been easier!

Incucyte^® Advanced Label-Free Classification Analysis Workflow shows the steps required to run a label-free live/dead assay. Images are automatically acquired and segmented using the Incucyte^® Cell-by-Cell Analysis or AI cell Health Analysis Software Modules.  With the Incucyte^® Advanced Label-Free Classification add-on, morphological features are obtained and a classifier is trained using live and dead control wells. This classifier is then applied to all wells and timepoints to achieve number and % class A and % class B cells per image.

• Make informed decisions on morphological changes with ease
• Combine with AI Cell Health Analysis for classification of accurately segmented live or dead cells
• Utilize integrated multivariate analysis and smart default settings for classifying morphological differences
• Track adherent cell populations kinetically and easily visualize changes in morphology
• Achieve consistent results with objective analysis using microplate formats, unlocking significant throughput

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