Incucyte® Cell-By-Cell Analysis Software Module

Incucyte® Cell-by-Cell Analysis Software

The Incucyte® Cell-by-Cell Analysis Software Module enables a range of applications for mixed cultures of of adherent and non-adherent cells. Perform label-free cell counts on adherent and non-adherent cells and subsequent cell-by-cell classification based on shape, size or fluorescence intensity to quantify dynamic changes in subpopulations within a mixed culture, opening a new world of discovery. 

Identification, classification, and analysis of subsets of cells is easily accomplished with a suite of purpose-built software tools and guided workflow.


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*Not available for the Incucyte® Zoom or FLR Live-Cell Analysis Systems


The Incucyte® Advanced Label-free Classification Analysis Software Module (Cat. No. BA-04867) is an add on module to Cell-by-Cell Analysis module and enables identification of label-free cells with morphologies of interest.

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Incucyte® Cell-by-Cell Analysis Workflow

Workflow indicates typical steps taken for use of the Incucyte® Cell-by-Cell Analysis Software Module to segment individual cells and classify population subsets using area, eccentricity or fluorescence intensity of labeled cells. Incucyte® Annexin V NIR reagent was used to detect total dead cell number in the presence of compound treatment over time. Additional functionality using total cell morphology can be accomplished using the Advanced Label-free Classification Analysis module.

Software Features

Identify, count and classify cells

  • Novel image segmentation algorithm identifies individual, unlabeled, adherent and non-adherent cells in HD phase images
  • One- or two-parameter classification tools group cells into subsets using size, shape or fluorescence intensity

Verify and graph changes in subsets over time

  • Visually verify classifications
  • Easily graph data to reveal trends and generate insight

Improve productivity

  • Define parameters once, then re-use or adjust
  • Automatically analyze and classify cells from thousands of images with a few clicks

Ordering information

Related Resources, Products, and Applications

1-Sheet: Incucyte® Morphological Analysis

Identify, classify and analyze subsets of label-free cells or cells exhibiting fluorescence with a suite of purpose-built software tools and guided workflows.

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Related Applications

Cell-by-Cell Analysis

Answer more questions about your adherent or non-adherent cell models with Incucyte® Cell-by-Cell Analysis.

Morphological Analysis 

Label-free cell counts and subsequent cell-by-cell classification of adherent or non-adherent cell models on area, eccentricity, or fluorescence inten...

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