Incucyte® 3D Object Classification Analysis Software Module (Cat. No. BA-04907)
The Incucyte® 3D Object Classification Analysis Software Module introduces a new level of analytical power for 3D cell culture research. This module enables researchers to automatically identify and classify populations of spheroids, organoids, and other complex 3D biological structures based on key morphological and functional characteristics—including shape, size, and fluorescence intensity.
Incucyte® 3D Object Classification Analysis Software Overview
Purpose-built for advanced 3D culture systems, the module streamlines image analysis workflows by combining object segmentation with automated classification, allowing users to visualize and quantify distinct subpopulations with ease. Interactive tools, such as color-coded masks, histograms, and density plots, link classification results directly back to raw images for deeper contextual understanding.
The 3D Object Classification analysis is exclusively compatible with images acquired using purchased Incucyte® Spheroid* Analysis Software Module (Cat. No. 9600-0019) or Incucyte® Organoid Analysis Software Module (Cat. No. 9600-0034), and can be applied to both newly acquired and previously captured datasets. By integrating seamlessly with these modules, it enhances experimental precision, accelerates discovery, and empowers researchers to uncover new insights into complex cellular dynamics over time.
*Spheroid – includes Embedded Multi-Spheroid and Multi-Spheroid on Top scan types.
Incucyte® 3D Object Classification Analysis Software is available for purchase for all Incucyte® CX3 and S-Series instruments and requires software version 2025C or higher.
Incucyte® 3D Object Classification Analysis Workflow
Figure 1: Typical workflow using the Incucyte® 3D Object Classification Analysis Software Module. 3D objects (spheroids and organoids) are segmented and classified into population subsets based on area, eccentricity, brightfield darkness, or fluorescence intensity of segmented objects. Incucyte® Caspase-3/7 Green reagent was used to detect apoptosis in Mouse Hepatic Organoids (expressing Nuclight Orange) in the presence of Camptothecin. Images show vehicle (top) versus Camptothecin-treated (3.3 µM, bottom) organoids at 48 hours.
Automated 3D Cell Culture Analysis for Deeper Biological Insights
Built to enhance high-content imaging workflows, the Incucyte® 3D Object Classification Analysis Software Module offers a streamlined approach to quantifying and comparing 3D culture characteristics. Researchers can efficiently analyze large datasets, visualize cell populations with flexible plotting tools, and extract meaningful trends across experimental conditions—all within the Incucyte® environment. This integration accelerates decision-making and supports more reproducible insights in cancer, organoid, and regenerative medicine research.
| Product | Qty. | Cat. No. |
| Incucyte® 3D Object Classification Analysis Software Module | 1 module | BA-04907 |
| available for Incucyte® CX3, SX5, S3, SX1 Live-Cell Analysis System. | ||
Incucyte® 3D Object Classification Analysis Software Features
Software Features
- Automated Object Classification – Segment and classify spheroids and organoids based on size, shape, brightfield darkness, and fluorescence intensity, eliminating the need for manual annotation.
- Customizable Subset Gating – Define and analyze specific object populations with flexible gating parameters, enabling precise evaluation of heterogeneous cultures.
- Dynamic Visualization Tools – Track changes over time using density plots and histograms, with color-coded classification masks directly linked to raw images for intuitive interpretation.
- Integrated Graphing – Quantify and compare phenotypic subsets using built-in analysis and customizable graphing tools to streamline data interpretation.
- Time-Lapse Tracking – Monitor subpopulation dynamics and functional changes across long-term experiments for deeper insight into 3D culture behavior.
- Streamlined Workflow – Navigate a guided, purpose-built interface that simplifies advanced 3D culture analysis while reducing workflow complexity.
