Incucyte® Spheroid Analysis Software Module (Cat. No. 9600-0019)
Analyze growth, viability and invasion of single spheroids in round-bottom, multi-well format or measure multi-spheroids in flat bottom plates to detect changes in growth and viability inside your tissue culture incubator.
Incucyte® Spheroid Analysis Software Overview
The Incucyte® Spheroid Analysis Software Module enables kinetic acquisition and objective assessment of spheroids within a physiologically relevant environment. Together with the Incucyte® CX3 Live-Cell Analysis System, featuring spinning disk confocal imaging, researchers are empowered to capture clear, kinetic 3D images of spheroids with minimal disruption to culture conditions—enabling richer and more consistent analysis over time. For widefield fluorescence and brightfield imaging, this software remains fully compatible with Incucyte® SX1, S3, and SX5 systems.
The integration of optimized protocols and Incucyte® software enables you to standardize your entire spheroid workflow, from generation, manipulation, and analysis, using imaged-based, label-free or fluorescence measurements. Characterize and assess spheroid growth or invasion and morphology during cultures and probe the effects of treatments through unbiased assessment of size, count and morphology. Incucyte® purpose built software automatically monitors and quantifies changes in two different assays models, depending on your scientific question:
Scaffold-free grown in round-bottom ULA plates
- Single spheroids tumor models to evaluate growth, shrinkage and death in 96- and 384-well formats
- Single spheroid invasion tumor model to quantitate invasive growth and shrinkage in a 96-well format
Learn more about Incucyte® Tumor Spheroid Assays and Spheroid Invasion
Scaffold-based grown in flat bottom plates
- Multi- spheroids (MS) grown on a layer of Matrigel® or embedded in Matrigel® on a base to quantify tumor spheroid formation, growth and health in a 96-well format.
Learn more about Incucyte® Multi-Spheroid Assays
| Additional Products | Qty. | Cat. No |
| Incucyte® Spheroid Analysis Software Module (Module available for Incucyte® SX5, S3, SX1, and CX3 Live-Cell Analysis System.) | 1 module | 9600-0019 |
| Incucyte® CX3 Live-Cell Analysis System | 1 instrument | Explore the CX3 |
| Incucyte® SX5 Live-Cell Analysis System | 1 instrument | 4816 |
Incucyte® Spheroid Analysis Software Features
Kinetically monitor and quantify spheroid changes overtime with purpose-built acquisition and analysis software
Figure 1: Process images of every well with easy to use guided interface (A) and assess experimental conditions using Incucyte® vessel view (B). Integrated kinetic analysis shows powerful insight for the characterization of advanced cell models (C).
Generate Reproducible, Quantitative Data
Lab-tested protocols for Single- or Multi-Spheroid Assays, high quality images, and unbiased analysis deliver robust data suitable for pharmacological analysis.
MCF-7
MS on a Layer of Matrigel® (10x magnification)
MDA-MB-231
MS on a Layer of Matrigel® (10x magnification)
MS Embedded in Matrigel® on a Base (4x magnification)
MS Embedded in Matrigel® on a Base (4x magnification)
Figure 2: Representative videos indicating label-free multi-spheroid growth and reveal differential spheroid morphologies over time. MCF-7 and MDA-MB-231 cells were seeded in media on a top of Matrigel® layer or embedded in Matrigel® on a top of base layer (1K cells/well) and allowed to form multi-spheroids (3 days). Time-lapse videos monitoring spheroid growth over time (0 – 7 days post formation) demonstrate morphological differences between round (MCF-7) and stellate (MDA-MB-231) multi-spheroids.
Learn more about Protocols and Assays: Incucyte® Tumor Spheroid Assays or Incucyte® Multi-Spheroid Assays
Unlock Your Productivity
Automatically acquire, analyze, and graph thousands of images from up to six 96- or 384-well plates in parallel and get to answer faster.
Figure 3: Perform robust, reproducible pharmacological analysis in physiologically relevant conditions. MCF-7-Nuclight red multi-spheroids were formed over 3 days prior to 7-day treatment with known cytotoxic compounds. Time-course plate views enable rapid visualization of treatment effects on both spheroid size (Total BF Area) and viability (red FLU Intensity within BF Boundary). Concentration response curves represent area under curve (AUC) analysis of the time-course data 0 - 7 days post-treatment. All compounds caused a concentration dependent inhibition of growth and viability with rank order of potency CMP > CHX > OXA.
Ordering Information
Additional Products
| Product | Qty. | Cat. No. |
|---|---|---|
| Incucyte® Nuclight Red Lentivirus (puro) 2.0 | 0.6 mL | BA-04887 |
| Incucyte® Nuclight Green Lentivirus (puro) 2.0 | 0.6 mL | BA-04888 |
| Incucyte® Nuclight Red Lentivirus (bleo) 2.0 | 0.6 mL | BA-04889 |
| Incucyte® Nuclight Green Lentivirus (bleo) 2.0 | 0.6 mL | BA-04890 |
| Incucyte® Nuclight Orange Lentivirus (puro) 2.0 | 0.6 mL | BA-04891 |
| Incucyte® Caspase-3/7 Green Dye | One vial (20 µL) | 4440 |
| Incucyte® Caspase 3/7 Red Dye | One vial (20 µL) | 4704 |
| Incucyte® Annexin V Red Dye | One vial (100 tests) | 4641 |
| Incucyte® Annexin V Green Dye | One vial (100 tests) | 4642 |
| Incucyte® Annexin V Orange Dye | One vial (100 tests) | 4759 |
| Incucyte® Annexin V NIR Dye | One vial (100 tests) | 4768 |
| Incucyte® Cytotox Red Reagent | Five vials (5 µL) | 4632 |
| Incucyte® Cytotox Green Reagent | Five vials (5 µL) | 4633 |
| Incucyte® Cytotox NIR Dye | One vial (100 µL) | 4846 |
