Stem Cell QC Non and Pluripotent Masks - purple and pink masks, Incucyte software screencapture

Incucyte® AI Stem Cell QC Analysis Software Module

Incucyte® AI Stem Cell QC Analysis Software Module (Cat. No. BA-04909)

Accelerate stem cell research with the Incucyte® AI Stem Cell QC Analysis Software Module - an AI-powered solution for label-free assessment of feeder-free pluripotent stem cell cultures. This purpose-built software automatically segments stem cell colonies and classifies them as pluripotent or non-pluripotent directly from HD phase-contrast images, eliminating the need for fluorescent labels, endpoint staining, or destructive quality control methods.

Designed to support routine stem cell culture monitoring and quality control, the module delivers objective, reproducible analysis with minimal user input. Researchers can evaluate colony growth, morphology, and pluripotency in real time while preserving valuable cell samples for downstream applications.

The Incucyte® AI Stem Cell QC Analysis Software Module is available for purchase for all Incucyte® CX3 and S-Series instruments running software version 2026B or higher.

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Incucyte® AI Stem Cell QC Analysis Software Overview

Automated, Label-Free Pluripotency Assessment

The Incucyte® AI Stem Cell QC Analysis Software Module leverages AI to automatically segment stem cell colonies and classify them as pluripotent or non-pluripotent from HD phase-contrast images. This purpose-built module provides a simple, non-destructive workflow for objective stem cell culture quality control. This module offers:

  • Label-free segmentation and classification of feeder-free pluripotent stem cell colonies
  • Automated identification of pluripotent and non-pluripotent colonies using pre-trained AI models
  • Objective and reproducible assessment of stem cell culture quality with minimal user input
  • Non-destructive evaluation that preserves valuable stem cell samples for downstream applications
  • Real-time monitoring of colony growth, morphology, and pluripotency throughout the experiment
  • Seamless integration into existing Incucyte® workflows for streamlined analysis and reporting
     

Incucyte® AI Stem Cell QC  workflow


 

Figure 1: The Incucyte® AI Stem Cell QC Analysis Software Module is an all-in-one analysis tool that segments stem cell colonies in HD phase-contrast images and classifies them as pluripotent or non-pluripotent. It allows users to perform analyses in real-time without the need for fluorescent labels or destructive protocols, generating accurate and objective data with minimal user input and reduced time requirements.  

ProductQtyCat. No.
Incucyte® AI Stem Cell QC Analysis Software Module1 ModuleBA-04909
available for Incucyte® CX3, SX5, S3, SX1 Live-Cell Analysis System.

Incucyte® AI Stem Cell QC Analysis Software Features

Automatically segments pluripotent stem cell colonies from HD phase-contrast images using purpose-built AI algorithms.

  • Classifies colonies as pluripotent or non-pluripotent within a single integrated analysis workflow
  • Generates colony-level metrics including area, shape, and growth characteristics for phenotypic profiling
  • Tracks colony growth and pluripotency changes longitudinally across the entire experiment
  • Supports fluorescence measurements within colony boundaries to enable multiparametric analysis
  • Visualizes segmentation and classification masks directly alongside source images for rapid result verification
  • Provides customizable graphs, image overlays, and data export options for streamlined reporting and downstream analysis
  • Delivers consistent, reproducible results through pre-trained neural networks without requiring user model training

 

 

Incucyte® AI Applications

Stem Cell QC Non and Pluripotent Masks - purple and pink masks, Incucyte software screencapture

Incucyte® Stem Cell QC Assays

Monitor stem cell pluripotency in real time with label-free live-cell imaging. Quantify iPSC colony growth, morphology, and quality control.

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Product Resources

Brochure: Incucyte® — Reagents, Consumables and Software
Brochure

Incucyte® — Reagents, Consumables and Software

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