CHO Cell Culture Media And Feeds
Chemically Defined, Animal Component-Free, and Ready-to-Use CHO Media
Chinese hamster ovary (CHO) cells are vital for therapeutic protein production in biopharmaceutical research and development and are consistently in high demand. Sartorius’ portfolio of CHO Cell Culture Media and Feeds includes a variety of high-performance, off-the-shelf culture media and feeds for CHO cell lines. The media and feeds are ideal for protein production using CHO-DG44, CHO-K1, CHO-S and CHO-GS cell lines and are suitable for research and routine large-scale GMP manufacturing.
Find the Right CHO Medium
CHO Media & Feeds Portfolio: Ready-to-Use for High Performance
The portfolio offers high performance, scalability, and high quality.
- Optimized for either stable or transient production
- 100% chemically defined, free of animal components, serum and hydrolysates
- Liquid and powder formulations available
- Little or no adaptation from other serum-free media needed
- Robust performance in batch, fed-batch and perfusion cultivations
- Customized solutions available
CHO Media Sample Kits
Sample media for recombinant proteins and monoclonal antibodies (mAbs).
Request Your Sample
4Cell® SmartCHO - All-in-one solution
4Cell® SmartCHO media and feeds are formulated to provide robust performances in batch/fed-batch and perfusion processes in various CHO clones (DG44, K1, S, and GS).
- Improved filterability and solubility
- High productivity and specificity
- High performance growth and protein yield
- Can be used in N-1 High-Inoculation Fed-Batch, Continuous perfusion, as well as clone selection in perfusion mimic
- Supporting Sartorius cell line development services in CHO-DG44 cell lines
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4Cell® CHO Medium TCX6D - One High-Performing Medium for Any CHO Clone
- For long-term, high-performance growth and protein yield of CHO cells
- Supports cell growth and production of recombinant proteins and antibodies in suspension culture
- Suitable for various CHO clones (e.g., DG44, K1, S) and other animal cell lines
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4Cell® CHOlean Medium - Lean and Robust Growth Medium for a Variety of Applications
- Lean medium formulation designed for batch and fed-batch processes
- Developed with minimum components to be lean, robust and flexible
- Free of L-glutamine, growth factors, serum, animal components and hydrolysates
- Supports cell growth and production of recombinant proteins and antibodies in suspension culture
- Suitable for various CHO clones
- Customization available
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4Cell® CHO Medium TC-42 - Workhorse Formulation Designed for CHO-DHFR- Cell Lines
- Growth medium suitable for various CHO cell lines
- Suitable from thawing, adaptation, growth and protein production — no need to use multiple media
- Available with added recombinant insulin, IGF, or without growth factor
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4Cell® CHO-GS TCX10D Medium w/o DS - For Long-Term High-Performance Growth with CHO-GS Cells
4Cell® CHO-GS TCX10D Medium w/o DS supports cell growth and production of recombinant proteins and antibodies in suspension culture.
- Suitable for CHO-GS cell lines and other CHO clones
- Contains no L-glutamine, serum, animal components or hydrolysates
- Can be used for research or manufacturing
- Customization is available
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4Cell® CHO TF Medium - Ideal Medium for Working With Transient CHO Cells
- Compatibility with cationic transfection reagents such as polyethylenimine facilitates transfection
- Does not contain L-glutamine, growth hormones, serum, animal components or hydrolysates
- Arginine and lysine are optional
- Minimum purchase amount 5L
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AdipoGen Life Sciences Testimonial
We were looking for chemically defined media to use with our CHO-K1 cells in suspension. After testing several Sartorius media, we selected 4Cell® SmartCHO for protein production. It has proven to be the best media used at Adipogen Lifesciences to date: it allowed cells to grow at a higher density compared to our current medium, resulting in a fourfold increase in protein yield. This makes it very useful for our stable clone production.
4Cell® CHO Feed Supplements
4Cell® Basic Feed
Basic Feed improves recombinant protein production in suspension culture by increasing cell density compared to batch processes.
- Chemically defined, serum-free, animal component-free and hydrolysate-free
- For use as a feeding solution in large-scale recombinant biopharmaceutical protein production fed-batch processes
- Highly concentrated nutrients increase the productivity of cultured cells
4Cell® CHO Feed TCX7D
4Cell® CHO Feed TCX7D is ideal for fed-batch processes, and, in combination with 4Cell® CHO-GS TCX10D Medium w/o DS, provides optimal nutrition for CHO-GS cells.
- Works with other CHO cell lines when L-glutamine is supplemented
- Supports stable growth of suspension cells at high viability in seed train culture
- Does not contain L-glutamine, animal-derived components, proteins or growth factors
- Contains hypoxanthine/thymidine
4Cell® SmartCHO Feeds
Ideal additives to 4Cell® SmartCHO Production Medium. 4Cell® SmartCHO Feed Medium A (FMA) and Feed Medium B (FMB) boost fed-batch productivity 3- to 5-fold compared to batch culture conditions.
- Available in powder and liquid
- Suitable for testing with the other Sartorius CHO media
4Cell® XtraCHO Media
4Cell® XtraCHO Media System was developed for our first generation of cell line development services. It is available for legacy applications needing continuity of supply.
FAQs - CHO Media
CHO cells are typically cultured in Ham's F12K or DMEM supplemented with 10% FBS. However, in commercially manufacturing, chemically defined and animal-derived component-free media are recommended for culturing Chinese hamster ovary (CHO) cell lines for consistency and quality assurance of the end product.
CHO cells became popular to study genetics because they have a low chromosome number. As a result, CHO cells have been used for a long time and we know a lot about their genetics and growth characteristics.
Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. Serum-free media are an important tool allowing scientists to culture cells with a defined set of conditions while minimizing variables.
Some advantages of using serum-free media include easier purification and downstream processing, precise evaluations of cellular function, increased definition, more consistent performance, and a more consistent supply chain.
