Comprehensive Cell Culture Media Solutions

For Your Cell-, Viral-, and Protein-Based Therapies​​.

Choose a winning culture for your therapy development. At Sartorius, we offer a wide range of media, raw materials, and services to meet your cell culture needs. Backed by a global manufacturing network, we provide what’s vital to your cells and your success - from screening to commercial production.

  • Find the right media, raw materials, and supporting services
  • Transition to GMP with compliant media and reagents
  • Scale seamlessly with flexible formats
  • Work with our dedicated technical and regulatory experts
  • Get consistent quality from our global manufacturing network

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A Winning Culture Combines What’s Vital to Your Cells — And Your Success.

Nourish

A winning culture nourishes, and our comprehensive media solutions portfolio cultivates cells for your modality. We offer a wide variety of media and raw materials, along with the technical and regulatory support needed to catalyze your therapy development. Start off strong by finding the right materials and supporting services from our extensive offering.

Deliver

A winning culture delivers the performance you need. Set your therapy development up for success with our proven and process-compatible media, raw materials, and services. We provide a variety of formats - powders and liquids in bottles, bags, and buckets - to help you overcome process development gaps quickly, scale seamlessly, and ensure robust performance.

Assure

A winning culture assures. Your commercial success depends on a reliable supply of high-quality materials, and our established global manufacturing and distribution network supports your needs. Sustain and scale up your manufacturing with consistent quality that minimizes product variability and meets relevant regulatory standards.

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Explore Solutions by Application

Media Solutions for Your Cell-, Viral-, and Protein-Based Therapies

Sartorius offers an extensive portfolio of high-quality media, raw materials, and services to support specialized cell types used in protein-based, viral-based, and cell- and gene-based applications.

Comprehensive Media Solutions for Your Cell-Based Therapy

Maximize cell quality attributes, ensure reproducible results, and reduce your development timelines. Whether you’re working in immune or stem cell therapies, we offer GMP-grade advanced therapy cell culture media, critical raw materials, as well as storage and preservation solutions to cover all major cell types.

T-Cell and Immunotherapy Media

Serum-free, xeno-free media for immune cells. Performance and reliability from research to clinic

Explore T-Cell Media

Stem Cell Media

Promote cell maintenance and health while ensuring a smooth transition to clinical applications.

Explore Stem Cell Media

Cell Biopreservation Solutions

Store a variety of cells with high-quality freezing and cold storage solutions.

Explore Biopreservation

Recombinant Human Albumin

A versatile stabilizer designed to overcome formulation challenges for cell-based therapy.

Explore Recombinant Albumin

Linkit® AX Aliquoting System

Fully closed aseptic system that distributes into ten bags with simultaneous and fast filling.

Explore Linkit® AX

Spent Media Analysis

Breakdown of analytes including amino acid (20+), trace metal (30+), and vitamin (10+) consumption.

Explore Analysis Portfolio

Preclinical and GMP Growth Factors and Cytokines

A large variety of preclinical and GMP growth factors and cytokines for ex vivo cell culture. 

Explore Growth Factors

Buffer Solutions

Buffers to optimize your downstream processes with standard buffer or a customized solution.

Explore Buffer Solutions

Comprehensive HEK Media, Raw Materials, and Services

Maximize titer production for your viral-based media with a complete upstream solution. We support most common cell line, reagent, and serotype combinations with our four chemically defined media, feed supplements, and reagents. Find solutions for your AAV, LVV, and vaccine development and manufacturing, along with custom media development, optimization, and characterization services.

HEK293 Media

For long-term, high-performance suspension culture in research and GMP manufacturing.

Explore HEK293 Media

Recombinant Human Albumin

A versatile stabilizer designed to overcome formulation challenges for gene therapy and vaccines.

Explore Recombinant Albumin

Spent Media Analysis

Breakdown of analytes including amino acid (20+), trace metal (30+), and vitamin (10+) consumption.

Explore Analysis Portfolio

Custom Media Development

Customized cell culture media and buffers based on existing formulations or improvement of recipes.

Explore Custom Media

Buffer Solutions

Buffers to optimize your downstream processes with standard buffer or a customized solution.

Explore Buffer Solutions

Comprehensive CHO Media, Raw Materials, and Services

Chinese hamster ovary (CHO) cells are ideal for therapeutic protein production in biopharmaceutical research and development. Our comprehensive offering of seven CHO cell culture media and four feeds, combined with our advanced cell line development and supporting media optimization services, enables simplified clone selection, optimized titer production, and seamless scale-up to your commercial needs.

CHO Cell Culture Media and Feeds

Chemically defined, high-performing, and off-the-shelf for various cell lines and applications.

Explore CHO Media

CHO Platform Services

Combine cell line development, cell banking, and testing services for protein expression.

Explore CHO Platform Services

Spent Media Analysis

Breakdown of analytes including amino acid (20+), trace metal (30+), and vitamin (10+) consumption.

Explore Analysis Portfolio

Custom Media Development

Customized cell culture media and buffers based on existing formulations or improvement of recipes.

Explore Custom Media

Buffer Solutions

Buffers to optimize your downstream processes with standard buffer or a customized solution.

Explore Buffer Solutions
icon-media-bottles
Test Cell Culture Media Sample

Sample for cell culture media and raw materials.
 

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Accelerated Global Capacity Expansion to Meet Your Manufacturing Needs

Investing in Our Global Manufacturing and Distribution Network

Discover how Sartorius facilitates global supply of quality materials through a growing, responsive global manufacturing and distribution network.

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Customized Solutions to Ease Your Scale-Up

Rely on our custom media and buffer manufacturing capabilities, complete with flexible packaging and shipping solutions.

Explore

Explore Media, Reagents, and Supplements

Specialty Media

Optimize cell proliferation and maintenance for protein-, viral-, and cell-based applications.

Explore Specialty Media

Stem Cell Media

Promote highly pluripotent and multipotent stem cells for research and clinical use.

Explore Stem Cell Media

Classical Media

Media designed to support growth and maintenance of a variety of cell lines.

Explore Classical Media

Custom Media Development

Customized cell culture media and buffers based on existing formulations or improvement of recipes.

Explore Custom Media

Reagents & Supplements

Optimize attachment, dissociation, growth, and propagation in controlled conditions.

Explore Reagents & Supplements

Microcarriers

Support adherent cell growth with animal protein-coated and animal component-free microcarriers.

Explore Microcarriers

Learn More About Cell Culture Media

Meet the Media – Our Series on High Quality Media

In this series, we talk to our media experts about the challenges our customers face in finding suitable media, feeds, and raw materials for their cell culture projects. 

Audiocast

4Cell® NutriT GMP Medium | Meet the Media Episode 1

In this episode, we talk to our media experts about the challenges in growing and activating T cells and how to overcome these challenges.

Listen Episode
Audiocast

CellGenix® GMP TCM | Meet the Media Episode 2

In this episode, we talk to our media experts about the applications and flexibility of this T-cell media.

Listen Episode
Audiocast

HEK 293 Cell Media Portfolio | Meet the Media Episode 3

We talk to our media experts about the challenges in gene therapies and gene-modified cell therapies and how your HEK293 media can help.

Listen Episode

Featured Assets

White Paper

Optimize Your Cell Culture Performance Through Spent Media Analytics

Accelerate media development, monitor nutrient consumption, optimize feeding strategies, and improve cell culture performance.

Download White Paper
eBook

A Comprehensive Guide to Finding the Right Cell Culture Media

In this eBook, our experts reveal valuable insights to help you develop an optimal cell culture media strategy for your bioprocess.

Download eBook
Publication

Advancing Bioprocessing With Automation and Digitalization

Continued advances in cell culture processing – learn how automation and digitalization are pushing bioprocessing forward.

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Article

Increasing Service With New Media Services

Sartorius recently acquired cell culture company Xell AG. We’re leveraging this acquisition to support our customers in the Indian market with increas...

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White Paper

Integrated Technologies to Accelerate Process Intensification for Vira...

PDF 1.1 MB
White Paper

Intensifying Upstream Processing and the Implications for Media Manage...

PDF 4.4 MB
Webinar

Cell and Gene Therapy - From Concept to Clinical Use

Watch Webinar
Brochure

Comprehensive Media Solutions for Your Protein-Based Therapy

Browse CHO media, feeds, and services that meet your needs. We provide what’s vital to your cells — and your success.

Download Brochure
Article

Mitigating Cell Culture Variability With Robust Screening of Raw Mater...

Learn how to Mitigate Cell Culture Variability with Robust Screening of Raw Materials and Adhere to GMP Guidelines.

Download Article

FAQs - Cell Culture Media

A primary cell line is produced from a single cell type isolated from tissue and cultured under optimal and controlled conditions. 

Cell medium ingredients include nutrients (both organic and inorganic), vitamins, salts, O2 and CO2 gas phases, proteins (from defined or undefined sources), carbohydrates, cytokines, growth factors, and cofactors.

Media should be changed every 2 to 3 days. However, media is best changed when cell density reaches a healthy level, so this also depends on the growth rate of your cell culture. Most media also contain a pH indicator that changes color to yellow when pH drops, which is a sign of nutrient depletion and waste accumulation.

  1. Serum-containing media - basal media supplemented with undefined biological materials such as serum or cell extracts.
  2. Serum-free, animal component-containing media - basal medium supplemented with defined proteins, growth factors, and cytokines from animal sources;
  3. Serum-free, xeno-free medium - basal media supplemented with defined proteins, growth factors, and cytokines from human origin only.
  4. Animal component-free, chemically defined medium - basal medium composed of material from chemically defined sources.

Cell culture medium provides nutrients and growth factors necessary for cell proliferation. A typical basal cell culture medium is composed of amino acids, carbohydrates, vitamins, inorganic salts, glucose, trace elements, and a buffer system to maintain a stable pH. Traditionally, animal-derived serum is used to supplement basal media to provide a source of growth factors. Now, serum-free, chemically defined formulations are highly favored.

Serum-free media are media designed to grow cells in the absence of animal-derived serum. Serum-free media include chemically defined media, which are free of any animal components or hydrolysates. 

Serum-free media allows cell culture to be performed under a defined set of conditions, limiting batch-to-batch variability. It also simplifies purification and downstream processing.

Xeno, derived from the Greek word “xenos”, means “foreigner.” A xeno-free media is a culture medium with a formulation that only contains human-derived components and not any other species such as bovine or porcine.

Serum-free media are free from any serum (from all animal species) but can include other animal components (such as HSA and BSA), while xeno-free is free of components from non-human animal species but can include human serum.

Each medium should be stored according to the manufacturer’s protocols and recommendations. Most basal media require storage under controlled conditions at 2-10°C, light protected, and secured to avoid degradation and contamination of the components. Once opened and supplemented with additional components under sterile conditions, the medium can typically be stored for 1-4 weeks at 2-8°C and light protected. 

Each medium should be stored according to the manufactured protocols and recommendations. The typical expiration date is within 12-18 months of manufacture. When open and supplemented with other components under sterile conditions, media can generally be stored for 1-4 weeks, at 2-8°C, and protected from light. Classical media supplemented with serum antibiotics can be kept at 4°C for up to 4 weeks; serum-free media can be kept for up to 2 weeks.

Bleach should be added to unused media at ≥10% final concentration to treat any microorganism present before disposal.

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