Sartorius Has the Right Specialty Media for Your Application

Optimized Media Improve Bioprocess Efficiency

Specialized cell types used in biotechnology research have diverse nutritional requirements that are not met by classical media. Sartorius offers a range of high-performing specialty media designed to optimize cell proliferation and maintenance. The portfolio of specialty media includes products designed for protein-based, viral-based, and cell-based applications. Sartorius’ specialty media are chemically defined of animal component-free and serum-free.

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CHO Cell Culture Media

Chemically-defined, high-performing, off-the-shelf culture media and feeds for various mammalian cell lines and applications.

Explore CHO Media

HEK Cell Culture Media

Ideal for long-term, high-performance suspension culture to produce adeno-associated viruses (AAV) and lentiviruses, viral vector production, as well as for gene therapy application.

Explore HEK293 Media

T Cell & Immunotherapy Cell Culture Media

Xeno-free, serum-free media for cultivating various types of immune cells and immunotherapy applications.

Explore 4Cell® Nutri-T Media

Sartorius Specialty Media Portfolio

Simplify Your Process With Sartorius Specialty Media

Sartorius offers a complete portfolio of high-quality media developed and optimized specifically for culturing specialized cell types used in viral-, protein- and cell-based applications. Sartorius media facilitate transfer from research to the clinic and simplify your processes, reduce risks and ease your regulatory procedures.

Specialty Media for Protein-Based Applications

Sartorius offers high-performance, off-the-shelf culture media and feeds optimized for Chinese Hamster Ovary (CHO) cell lines. The media and feeds are designed to enhance protein production using CHO-DG44, CHO-K1, CHO-S and CHO-GS cell lines and are suitable for research and routine large-scale GMP manufacturing.

  • Chemically defined
  • Animal component-free
  • Serum-free

TCX6D

TC-42

CHOlean

TCX10D

CHO TF

CHO-DG44

 

  

 

 

CHO-K1

   

 

 

CHO-S

   

 

 

CHO-GS

   

For stable expression

    

For transient production

 

 

 

 

 

Volume

10L-500L

1L – 50L

1L – 100L

1L– 500L

1L – 50L

Packaging

Bottle; Bucket 

Bottle; Bucket 

Bottle; Bucket 

Bottle; Bucket 

Bottle; Bucket 

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Specialty Media for Viral-Based Applications

Sartorius offers a complete portfolio of media and feed to produce viral vaccines or viral vectors for vaccines, gene therapy and gene-modified cell therapy applications

  • Chemically defined
  • Animal component-free
  • Serum-free

HEK293 Media

MDCK Medium

Vero Medium

Insect Medium

BHK-21 Medium

Available in liquid

1L - 50L*

1L*

0,5L – 200L

50L**

0,5L*

Available in
powder

5L - 500L

10L – 100L

10L – 500L**

 

10L – 100 L

For vaccine
applications

 

 

 

 

 

For viral vector
applications

 

 

  
(Oncolytic Virus)

 

 

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More volumes upon request*

Available in the future **

Specialty Media for Cell and Gene Therapy Applications

Enhance cell therapies with specialty media systems to optimize the culture of immune and stem cells. Whether conducting basic research, pre-clinical or clinical trials, trust Sartorius’ expertise to accompany and support you. Sartorius offers high-quality media, technical and regulatory support and documentation, allowing an easy and seamless transition from research to clinic and commercialization.

  • Serum-free, xeno-free formulations
  • Manufactured in cGMP-compliant facility
4Cell® Nutri-T MediaNutriStem® hPSC XF MediaMSC NutriStem® XF Media

EndoGo™ XF Media

Cell Type

Immune cells

Human pluripotent stem cells

Human mesenchymal stem/ stromal cells

Endothelial Cells

DMF

Pending

Available

Available

 

Volume

1L*

0.5L - 10L*

0.5L – 20L*

0.5L*

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* More volumes upon request

Speciaty Media for Viral-Based Applications

High-Performing Specialty Media for Viral-Based Applications

Sartorius’ specialty media for viral-based therapeutics support:

  • Viral Vaccines | Influenza, FMD, Covid-19
  • Viral Vector Vaccines (including oncolytic virus) | Adenovirus
  • Gene Therapy | AAV
  • Gene Modified Cell Therapy Vectors | Lentivirus

Sartorius’ specialty media for virus production are chemically-defined, animal component-free, serum-free and hydrolysate-free.


High-Performing Specialty Media for HEK293 Cell Lines

Sartorius offers four high-performing suspension HEK media that are designed for use with the high variability of HEK293 cell lines, the versatility of adeno-associated virus and complexity of viral vector processes. The portfolio also includes a unique feed supplement to boost production of viral vectors and virus for gene therapy and vaccines.

Explore HEK293 Media

4Cell®MDCK Medium: One Ready-to-Use Medium for All Upstream Steps

4Cell® MDCK CD is a ready-to-use specialty medium designed to support Madin-Darby canine kidney (MDCK) cell growth and infection in suspension for both research and manufacturing applications.

  • Chemically defined
  • Serum-free
  • Protein-free
  • Animal component-free
  • Hydrolysate-free
  • Available in liquid and powder format

Explore MDCK Media

Next-Gen Vero Media for Consistent Results, Maximum Control

The chemically defined 4Cell® NutriVeroFlex 10 Medium is developed with ultrapure inorganic and organic ingredients, without animal components or any plant, yeast extract or peptone.

  • Increase the safety of biological products
  • Facilitate downstream product purification
  • Consistently provide superior cell growth and enhance virus titer productivity for vaccines and oncolytic manufacturing

Explore Vero Media

BHK-21 Media

The 4Cell® BHK-21 CD Medium is a complete medium designed and to reduce costly product purification steps of BHK to support high-density growth and maintenance and suspension cell lines used for viral vaccine production.

  • Ready-to-use
  • Chemically defined
  • Serum-free
  • Animal component-free
  • Antibiotic-free
  • Hydrolysate-free

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Insect Media

The 4Cell® Insect CD Medium is a complete medium designed to support the growth of insect cell lines derived from Spodoptera frugiperda for vaccines applications (BEVS).

  • Chemically defined
  • Serum-free
  • Protein-free
  • Animal component-free
  • Hydrolysate-free

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Specialty Media for Protein-Based Therapeutics

CHO Cell Culture Media Portfolio — Ready-to-Use for High Protein Yields

The Sartrorius CHO media and feeds are developed for long-term, high-performance suspension cultures. They are suitable for research and large-scale GMP manufacturing.

  • 100% chemically defined and animal component-free
  • Designed as all-in-one solutions: thawing, expansion and production
  • Little or no adaption required from other serum-free media
  • Optimized for stable or transient production
  • Available as liquid and powder formulations
  • Customized solutions are available on demand


TCX6D — One High-Performing Medium for Any CHO Clone

  • For long-term, high-performance growth and protein yield
  • Supports cell growth and production of recombinant proteins and antibodies in suspension culture
  • Suitable for various CHO clones (e.g., DG44, K1, S) and other animal cell lines

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TC-42 —Optimized for CHO-DHFR- Cell Line

TC-42 Medium is the optimal medium for various CHO processes.

  • Supports stable growth of suspension cells at high viability in seed train culture
  • Available with IGF or recombinant insulin, or without growth factor
  • Customization available for SILAC/deficiency variant

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CHOlean — Lean and Robust Growth Medium for a Variety of Applications

  • Lean medium formulation designed for batch and fed-batch processes
  • Developed with minimum components to be lean, robust and flexible
  • Free of L-glutamine, growth factors, serum, animal components and hydrolysates
  • Supports cell growth and production of recombinant proteins and antibodies in suspension culture
  • Suitable for various CHO clones
  • Customization available

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TCX10D — For Long-Term High-Performance Growth With CHO-GS Cells

TCX10D medium supports cell growth and production of recombinant proteins and antibodies in suspension culture.

  • Suitable for CHO-GS cell lines and other CHO clones
  • Contains no L-glutamine, serum, animal components or hydrolysates
  • Can be used for research or manufacturing
  • Customization is available
  • Little or no adaptation required from other serum-free media

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CHO TF — Ideal Medium for Working With Transient CHO Cells

  • Suitable for various CHO clones (CHO-DG44, CHO-K1, CHO-S, CHO-GS)
  • For transfection applications and virus production
  • Supports cell growth and production of recombinant proteins and antibodies in high-density suspension culture
  • Compatibility with cationic transfection reagents (e.g. PEI) facilitates transfection
  • Does not contain L-glutamine or growth hormones
  • Arginine and lysine are optional

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Specialty Media for Cell and Gene Therapy Applications

Choose the Right Specialty Media for Your Cell and Gene Therapy Application

Sartorius’ specialty media support cell expansion and activation for immunotherapy and stem cell applications. Sartorius offers high-quality media, developed and optimized specifically for culturing stem cells, PBMCs, T cells, CARTs and TILs. With the serum-free, xeno-free NutriStem® family and 4Cell® Nutri-T Media, you can simplify your process, reduce the risks associated with serum, and ease your regulatory procedures.


Specialty Media for T Cells and Immunotherapy Applications

4Cell® Nutri-T media are ideal for the development and scale-up of immune cell-based therapeutic applications.

  • Optimized for T cell, TIL, CAR-T and PBMC applications
  • Serum-free, xeno-free media
  • Excellent performance without the addition of human serum
  • cGMP manufactured
  • DMF available
  • Seamless transfer from research to the clinic

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Specialized Stem Cell Media

NutriStem® xeno-free culture media support high-quality cell maintenance and cell health, allowing smooth transfers to clinical applications.

  • Optimize for either hMSC or hiPSC, and hESC
  • High proliferation rate, long-term growth and differentiation ability
  • Complete protocols, application data and scientific references
  • DMF available
  • cGMP manufactured

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Freezing Media and Cryopreservation Solutions

NutriFreez® cryopreservation solutions are optimized to protect a wide range of cell types such as primary cells, lymphocytes, and stem cells, while assuring enhanced viability and high recovery rates.

  • Serum-free, animal component-free, protein-free, and fully defined formulations
  • Widely referenced
  • cGMP manufactured
  • Suitable for clinical applications
  • DMF available

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Learn More About Specialty Media

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How a Single Provider Can Reduce Protein Biologic Development Risk

In this webinar, Sartorius and Sonnet BioTherapeutics discuss the benefits of the Sartorius CHO Platform of integrated products and services.

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A Comprehensive Guide to Finding the Right Cell Culture Media

In this eBook, our experts reveal valuable insights to help you develop an optimal cell culture media strategy for your bioprocess.

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