Sartorius Has the Right Specialty Media for Your Application
Optimized Media Improve Bioprocess Efficiency
Specialized cell types used in biotechnology research have diverse nutritional requirements that are not met by classical media. Sartorius offers a range of high-performing specialty media designed to optimize cell proliferation and maintenance. The portfolio of specialty media includes products designed for protein-based, viral-based, and cell-based applications. Sartorius’ specialty media are chemically defined of animal component-free and serum-free.
Highlight Products
CHO Cell Culture Media
Chemically-defined, high-performing, off-the-shelf culture media and feeds for various mammalian cell lines and applications.
HEK Cell Culture Media
Ideal for long-term, high-performance suspension culture to produce adeno-associated viruses (AAV) and lentiviruses, viral vector production, as well as for gene therapy application.
T Cell & Immunotherapy Cell Culture Media
Xeno-free, serum-free media for cultivating various types of immune cells and immunotherapy applications.
Sartorius Specialty Media Portfolio
Simplify Your Process With Sartorius Specialty Media
Sartorius offers a complete portfolio of high-quality media developed and optimized specifically for culturing specialized cell types used in viral-, protein- and cell-based applications. Sartorius media facilitate transfer from research to the clinic and simplify your processes, reduce risks and ease your regulatory procedures.
Specialty Media for Protein-Based Applications
Sartorius offers high-performance, off-the-shelf culture media and feeds designed for batch, fed batch and perfusion processes with different Chinese Hamster Ovary (CHO) cell lines. The Sartorius CHO Media and Feeds support cell growth and production of recombinant proteins and antibodies in suspension culture.
They are suitable for research and in manufacturing applications:
- Chemically defined solutions
- Animal component-free
- Protein-free
- Serum-free
4Cell® | TCX6D | CHOlean | TC-42 | TCX10D | 4Cell® | CHO TF | |
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Volume | 9 L - 150 L | 10 L - 500 L | 1 L - 100 L | 1 L - 50 L | 1 L - 500 L | 10 L - 250 L | 1 L - 50 L |
Packaging | Bottle; | Bottle; Bucket | Bottle; Bucket | Bottle; Bucket | Bottle; Bucket | Bucket | Bottle; Bucket |
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Specialty Media for Viral-Based Applications
Sartorius offers a complete portfolio of media and feed to produce viral vaccines or viral vectors for vaccines, gene therapy and gene-modified cell therapy applications
- Chemically defined
- Animal component-free
- Serum-free
HEK293 Media | MDCK Medium | Vero Medium | Insect Medium | BHK-21 Medium | |
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Available in liquid | 1L - 50L* | 1L* | 0,5L – 200L | 50L** | 0,5L* |
Available in | 5L - 500L | 10L – 100L | 10L – 500L** |
| 10L – 100 L |
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More volumes upon request*
Available in the future **
Specialty Media for Cell and Gene Therapy Applications
Enhance cell therapies with specialty media systems to optimize the culture of immune and stem cells. Whether conducting basic research, pre-clinical or clinical trials, trust Sartorius’ expertise to accompany and support you. Sartorius offers high-quality media, technical and regulatory support and documentation, allowing an easy and seamless transition from research to clinic and commercialization.
- Serum-free, xeno-free formulations
Manufactured in accordance with applicable cGMP guidelines
| 4Cell® Nutri-T Media | NutriStem® hPSC XF Media | MSC NutriStem® XF Media | EndoGo™ XF Media | CellGenix® | CellGenix® GMP DC Medium | CellGenix® GMP | |
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Cell Type | Immune cells | Human pluripotent stem cells | Human mesenchymal stem/ stromal cells | Endothelial cells | HSCs, NK cells, CIK cells | Dendritic cells | T cells, CAR-Ts, Tregs |
DMF | Pending | Available | Available |
| Available | Available | Pending |
Volume | 1L* | 0.5L - 10L* | 0.5L – 20L* | 0.5L* | 0.5L | 0.5L | 0.5 L*, 1L Pending |
| Learn More | Learn More | Learn More |
* More volumes upon request
Speciaty Media for Viral-Based Applications
High-Performing Specialty Media for Viral-Based Applications
Sartorius’ specialty media for viral-based therapeutics support:
- Viral Vaccines | Influenza, FMD, Covid-19
- Viral Vector Vaccines (including oncolytic virus) | Adenovirus
- Gene Therapy | AAV
- Gene Modified Cell Therapy Vectors | Lentivirus
Sartorius’ specialty media for virus production are chemically-defined, animal component-free, serum-free and hydrolysate-free.
High-Performing Specialty Media for HEK293 Cell Lines
Sartorius offers four high-performing suspension HEK media that are designed for use with the high variability of HEK293 cell lines, the versatility of adeno-associated virus and complexity of viral vector processes. The portfolio also includes a unique feed supplement to boost production of viral vectors and virus for gene therapy and vaccines.
4Cell®MDCK Medium: One Ready-to-Use Medium for All Upstream Steps
4Cell® MDCK CD is a ready-to-use specialty medium designed to support Madin-Darby canine kidney (MDCK) cell growth and infection in suspension for both research and manufacturing applications.
- Chemically defined
- Serum-free
- Protein-free
- Animal component-free
- Hydrolysate-free
- Available in liquid and powder format
Next-Gen Vero Media for Consistent Results, Maximum Control
The chemically defined 4Cell® NutriVero™ Flex 10 Medium is developed with ultrapure inorganic and organic ingredients, without animal components or any plant, yeast extract or peptone.
- Increase the safety of biological products
- Facilitate downstream product purification
- Consistently provide superior cell growth and enhance virus titer productivity for vaccines and oncolytic manufacturing
BHK-21 Media
The 4Cell® BHK-21 CD Medium is a complete medium designed and to reduce costly product purification steps of BHK to support high-density growth and maintenance and suspension cell lines used for viral vaccine production.
- Ready-to-use
- Chemically defined
- Serum-free
- Animal component-free
- Antibiotic-free
- Hydrolysate-free
Insect Media
The 4Cell® Insect CD Medium is a complete medium designed to support the growth of insect cell lines derived from Spodoptera frugiperda for vaccines applications (BEVS).
- Chemically defined
- Serum-free
- Protein-free
- Animal component-free
- Hydrolysate-free
Specialty Media for Protein-Based Therapeutics
CHO Cell Culture Media Portfolio — Ready-to-Use for High Protein Yields
The Sartorius CHO media and feeds are developed for long-term, high-performance suspension cultures. They are suitable for research and in manufacturing applications.
- 100% chemically defined and animal component-free
- Designed for thawing, expansion, and production
- Little or no adaption required from other serum-free media
- Optimized for stable or transient production
- Available as liquid and powder formulations
- Customized solutions are available on demand
4Cell® SmartCHO - All-in-one solution
4Cell® SmartCHO media and feeds are formulated to provide robust performances in batch/fed-batch processes in various CHO clones (DG44, K1, S, and GS).
- Improved filterability and solubility
- High productivity and specificity
- High performance growth and protein yield
- Supporting Sartorius 4Cell® CHO Platform cell line development services in CHO-DG44 cell lines
4Cell® SmartCHOpe - A CHO perfusion medium
4Cell® SmartCHOpe media and feeds are designed for perfusion processes and support intensified process formats.
- Can be used in high-inoculation fed batch
- Ideal for N-1 Perfusion and continuous perfusion
- Support clone selection in perfusion mimic protocol
TCX6D — One High-Performing Medium for Any CHO Clone
- For long-term, high-performance growth and protein yield
- Supports cell growth and production of recombinant proteins and antibodies in suspension culture
- Suitable for various CHO clones (e.g., DG44, K1, S) and other animal cell lines
TC-42 —Optimized for CHO-DHFR- Cell Line
TC-42 Medium is the optimal medium for various CHO processes.
- Supports stable growth of suspension cells at high viability in seed train culture
- Available with IGF or recombinant insulin, or without growth factor
- Customization available for SILAC/deficiency variant
CHOlean — Lean and Robust Growth Medium for a Variety of Applications
- Lean medium formulation designed for batch and fed-batch processes
- Developed with minimum components to be lean, robust and flexible
- Free of L-glutamine, growth factors, serum, animal components and hydrolysates
- Supports cell growth and production of recombinant proteins and antibodies in suspension culture
- Suitable for various CHO clones
- Customization available
TCX10D — For Long-Term High-Performance Growth With CHO-GS Cells
TCX10D medium supports cell growth and production of recombinant proteins and antibodies in suspension culture.
- Suitable for CHO-GS cell lines and other CHO clones
- Contains no L-glutamine, serum, animal components or hydrolysates
- Can be used for research or manufacturing
- Customization is available
- Little or no adaptation required from other serum-free media
CHO TF — Ideal Medium for Working With Transient CHO Cells
- Suitable for various CHO clones (CHO-DG44, CHO-K1, CHO-S, CHO-GS)
- For transfection applications and virus production
- Supports cell growth and production of recombinant proteins and antibodies in high-density suspension culture
- Compatibility with cationic transfection reagents (e.g. PEI) facilitates transfection
- Does not contain L-glutamine or growth hormones
- Arginine and lysine are optional
Specialty Media for Cell and Gene Therapy Applications
Choose the Right Specialty Media for Your Cell and Gene Therapy Application
Sartorius’ specialty media support cell expansion and activation for immunotherapy and stem cell applications. Sartorius offers high-quality media, developed and optimized specifically for culturing stem cells, PBMCs, T cells, CARTs and TILs. With the serum-free, xeno-free NutriStem® family, 4Cell® Nutri-T Media, and CellGenix® family, you can simplify your process, reduce the risks associated with serum, and ease your regulatory procedures.
Specialty Media for T Cells and Immunotherapy Applications
4Cell® Nutri-T media are ideal for the development and scale-up of immune cell-based therapeutic applications.
- Optimized for T cell, TIL, CAR-T and PBMC applications
- Serum-free, xeno-free media
- Excellent performance without the addition of human serum
Manufactured in accordance with applicable cGMP guidelines
- DMF available
- Seamless transfer from research to the clinic
Specialized Stem Cell Media
NutriStem® xeno-free culture media support high-quality cell maintenance and cell health, allowing smooth transfers to clinical applications.
- Optimize for either hMSC or hiPSC, and hESC
- High proliferation rate, long-term growth and differentiation ability
- Complete protocols, application data and scientific references
- DMF available
Manufactured in accordance with applicable cGMP guidelines
Freezing Media and Cryopreservation Solutions
NutriFreez® cryopreservation solutions are optimized to protect a wide range of cell types such as primary cells, lymphocytes, and stem cells, while assuring enhanced viability and high recovery rates.
- Serum-free, animal component-free, protein-free, and fully defined formulations
- Widely referenced
- Manufactured in accordance with applicable cGMP guidelines
- Suitable for clinical applications
- DMF available
CellGenix® GMP Dendritic Cell Medium
DCs are used for cancer immunotherapy. CellGenix® GMP DC is an optimized, serum-free medium used for the generation of human dendritic cells (DCs).
- Optimized formulation for the generation of DCs
- GMP manufactured
- FDA DMF available
Specialty Media for NK Cells
CellGenix® GMP SCGM is an optimized, xeno-free medium for the expansion and cultivation of human natural killer cells (NK cells).
- Expansion and differentiation of NK cells and CIK cells
- Serum-free cultivation of HSCs
- GMP manufactured
- FDA & PMDA DMF available
Learn More About Specialty Media
FAQ - Specialty Media
- Serum containing media — basal media supplemented with undefined materials as serum, cells extracts, and more
- Serum-free, animal component-containing medium — basal medium supplemented with defined proteins, growth factors, cytokines, and more from animal sources
- Serum-free, xeno-free basal medium supplemented with defined proteins, growth factors, cytokines, and more from human sources only
- Animal component-free, chemically defined medium — basal medium from chemically defined sources
