Protect Your Cells Through Freeze and Thaw

Cell Freezing Media and Salt-Based Solutions

High-Performance Protection for Cell Freezing and Storage

NutriFreez® cell freezing solutions are designed and validated for the cryopreservation of cells including stem cells, T cells, and PBMCs. They support animal component-free conditions during cryopreservation, essential to maintaining consistency when culturing cells in xeno-free and animal component-free systems. With proven reliability and consistency, NutriFreez® provides a high-performance protective environment for cells during the freezing, storage, and thawing processes. 

Find Your Cryopreservation Solution

NutriFreez® Cryopreservation Solutions 

  • Optimized to protect a wide range of human and animal-derived cells including primary cells, lymphocytes and stem cells in temperatures as low as -196°C. 

  • Enhanced cell viability and recovery 

  • Unique, serum-free, fully defined formulations 

  • Widely referenced 

  • GMP manufactured  

  • Suitable for clinical applications — salt-based and reduced DMSO concentration solution is available 

  • DMF available 

View Products

Media Purchasing Made Easy

Select your media type and benefit from our simplified shopping experience.

Shop NutriFreez® Medium Now

Choose the Optimal Freezing Media for Your Cells

NutriFreez® D10 Cryopreservation Media effectively maintain cell viability, adhesion, and bioactivity in various cell types including stem cells, T cells, PBMCs, CHO, and Vero cells. 

  • Contains 10% DMSO 

  • Animal component-free 

  • Protein-free 

  • Ready-to-use solution, simple protocol 

  • GMP manufactured 

  • Drug Master File (DMF) available 

View Products

NutriStem® D10 Performance Data

Maximum hMSC Proliferation with Normal Morphology

hMSCs in NutriFreez® D10 Cryopreservation Medium exhibit normal morphology and exceptional proliferation, compared to other commercial serum-free solutions, three days post-thaw. 

High hMSC Viability Rate After Long-Term Freezing

NutriFreez® D10 Cryopreservation Medium yields greater than 91% viability after long-term cryopreservation

High Recovery of hPSCs

Single-cell recovery, morphology, and attachment of human induced pluripotent stem cells post cryopreservation in NutriFreez® D10 Cryopreservation Medium 

High Viability of PBMCs

PBMCs cryopreserved in NutriFreez® D10 show ≥ 91% viability when compared to cells cryopreserved in homebrew freezing solutions

Choose the Optimal Freezing Solution for Cell Therapies 

NutriFreez® D5 Salt-Based Cryopreservation is the optimal solution for the freezing and thawing of cells intended for cell therapies and clinical applications. 

  • Contains 5% DMSO 

  • Animal component-free 

  • Protein-free 

  • Ready-to-use solution, simple protocol 

  • GMP manufactured 

  • High viability, recovery rates, and performance, even for extremely sensitive cells 

View Products

NutriStem® D5 Performance Data

High Viability of hMSCs from Various Sources

hMSCs derived from a variety of sources were frozen in NutriFreez® D5 Solution and thawed after one week.

High Proliferation and Normal Morphology of hMSCs

hMSCs derived from varied sources were frozen in NutriFreez® D5 Solution. Representative images (X100) taken 3 days post seeding show the proliferation results. Numbers represent viable cell counts.

Maintenance of hMSC Classical Marker Profile

hMSCs maintain classical profile of MSC markers with low percentage of hematopoietic contamination after being frozen in NutriFreez® D5 Salt-Based Cryopreservation Solution

Maintenance of hMSC Multipotency

AT-MSCs cells were cryopreserved in NutriFreez® D5 Salt Based Solution, thawed at P2 and, for passage P3/P4, seeded and grown in adipogenic, osteogenic and chondrogenic MSCgo™ Differentiation Media.

Featured Assets

Webinar

Optimized and Efficient Cell Cryopreservation

Watch the webinar to learn how to master cryopreservation.

Watch Webinar
Lecture Poster

Evaluation of Different Cryopreservation Agents for hMSC

Determining the optimal solution for preserving the viability of MSCs.

Read Now

Learn More About NutriFreez® Cryopreservation Solutions

Choosing Cell Freezing Media for Research and Cell Therapy

  • Optimized for maximum cell viability and recovery, NutriFreez® protects a wide range of cells, including highly sensitive cell types such as primary cells, lymphocytes, T cells, and stem cells (hMSCs and hPSCs).
  • Unique, high-quality, serum-free, animal component-free, protein-free, fully defined freezing formulations are ideal for research, cell banking, and therapeutic applications.
  • Salt-based solution with only 5% DMSO concentration is available and appropriate for cell therapy applications.
  • Manufactured in compliance with cGMP guidelines.
  • DMF available.
  • Referenced by more than 300 publications.
  • Customization and scale-up support is available.

Complete Your Application

Stem Cell Culture Media and Reagents

High-quality serum-free stem cell culture media to support the reproducible generations of quality-assured and clinically acceptable stem cells.

Learn More

T Cell and Immunotherapy Media

Xeno-free, serum-free media for the cultivation of immune cells offering superior performance and reliability from research to clinic

Learn More

Cell Booster Bundle

Build your ideal bundle for cell culture with our selection of media, growth factors, cytokines, and microcarriers.

Explore Cell Booster

Related Assets

User Guide

Cellular Cryopreservation Basics for Research and Clinical Use

PDF | 2.2 MB
Poster

Evaluation of Different Cryopreservation Agents for Mesenchymal Stem Cell as Final Study Product

PDF | 490.8 KB

Consult Our Experts

Consult Our Experts

Related Topics

Cell and Gene Therapy

Learn more about Sartorius' contribution.

Learn more

Celsius® CFT Platform

Celsius® CFT technology is a controlled-rate freezing and thawing system designed to minimize cryoconcentration. 

Explore More

Cell Banking

Learn More