Cell Freezing Media and Salt-Based Solutions
High-Performance Protection for Cell Freezing and Storage
NutriFreez® cell freezing solutions are designed and validated for the cryopreservation of cells including stem cells, T cells, and PBMCs. They support animal component-free conditions during cryopreservation, essential to maintaining consistency when culturing cells in xeno-free and animal component-free systems. With proven reliability and consistency, NutriFreez® provides a high-performance protective environment for cells during the freezing, storage, and thawing processes.
Find Your Cryopreservation Solution
NutriFreez® Cryopreservation Solutions
Optimized to protect a wide range of human and animal-derived cells including primary cells, lymphocytes and stem cells in temperatures as low as -196°C.
Enhanced cell viability and recovery
Unique, serum-free, fully defined formulations
Widely referenced
GMP manufactured
Suitable for clinical applications — salt-based and reduced DMSO concentration solution is available
DMF available
Choose the Optimal Freezing Media for Your Cells
NutriFreez® D10 Cryopreservation Media effectively maintain cell viability, adhesion, and bioactivity in various cell types including stem cells, T cells, PBMCs, CHO, and Vero cells.
Contains 10% DMSO
Animal component-free
Protein-free
Ready-to-use solution, simple protocol
GMP manufactured
Drug Master File (DMF) available
NutriStem® D10 Performance Data
Maximum hMSC Proliferation with Normal Morphology
hMSCs in NutriFreez® D10 Cryopreservation Medium exhibit normal morphology and exceptional proliferation, compared to other commercial serum-free solutions, three days post-thaw.
High hMSC Viability Rate After Long-Term Freezing
NutriFreez® D10 Cryopreservation Medium yields greater than 91% viability after long-term cryopreservation
High Recovery of hPSCs
Single-cell recovery, morphology, and attachment of human induced pluripotent stem cells post cryopreservation in NutriFreez® D10 Cryopreservation Medium
High Viability of PBMCs
PBMCs cryopreserved in NutriFreez® D10 show ≥ 91% viability when compared to cells cryopreserved in homebrew freezing solutions
Choose the Optimal Freezing Solution for Cell Therapies
NutriFreez® D5 Salt-Based Cryopreservation is the optimal solution for the freezing and thawing of cells intended for cell therapies and clinical applications.
Contains 5% DMSO
Animal component-free
Protein-free
Ready-to-use solution, simple protocol
GMP manufactured
High viability, recovery rates, and performance, even for extremely sensitive cells
NutriStem® D5 Performance Data
High Viability of hMSCs from Various Sources
hMSCs derived from a variety of sources were frozen in NutriFreez® D5 Solution and thawed after one week.
High Proliferation and Normal Morphology of hMSCs
hMSCs derived from varied sources were frozen in NutriFreez® D5 Solution. Representative images (X100) taken 3 days post seeding show the proliferation results. Numbers represent viable cell counts.
Maintenance of hMSC Classical Marker Profile
hMSCs maintain classical profile of MSC markers with low percentage of hematopoietic contamination after being frozen in NutriFreez® D5 Salt-Based Cryopreservation Solution
Maintenance of hMSC Multipotency
AT-MSCs cells were cryopreserved in NutriFreez® D5 Salt Based Solution, thawed at P2 and, for passage P3/P4, seeded and grown in adipogenic, osteogenic and chondrogenic MSCgo™ Differentiation Media.
Featured Assets
Learn More About NutriFreez® Cryopreservation Solutions
Choosing Cell Freezing Media for Research and Cell Therapy
- Optimized for maximum cell viability and recovery, NutriFreez® protects a wide range of cells, including highly sensitive cell types such as primary cells, lymphocytes, T cells, and stem cells (hMSCs and hPSCs).
- Unique, high-quality, serum-free, animal component-free, protein-free, fully defined freezing formulations are ideal for research, cell banking, and therapeutic applications.
- Salt-based solution with only 5% DMSO concentration is available and appropriate for cell therapy applications.
- Manufactured in compliance with cGMP guidelines.
- DMF available.
- Referenced by more than 300 publications.
- Customization and scale-up support is available.
