HEK293 Media & Feed Portfolio
High-Performing Specialty Media and Feed for HEK293 Cell Lines
Growth in gene therapies and viral vector vaccines has increased the demand for robust HEK293 cell lines. Sartorius offers four high-performing suspension HEK media that are designed for use with the high variability of HEK293 cell lines, the versatility of adeno-associated virus and the complexity of viral vector processes. The portfolio also includes a unique feed supplement to boost viral production for gene therapies and vaccines.
Unique HEK Media for Your Unique Cells
HEK Media & Feed Portfolio for Optimized Growth
- High performance, scalability and quality, ensuring purity and safety of the final product
- Optimized for transient transfection and infection or stable expression in suspension
- 100% chemically defined, animal component-free, serum-free
- Available in liquid and powder formats
- Requires little or no adaptation from other serum-free media
- Manufactured under ISO 9001 & ISO 13485 quality standards
Sample media for gene therapy and viral vector vaccines.
HEK GM
Robust and Effective Base Medium for Infection and Stable Expression
- Developed for high-performance infection of HEK293 and other human cell lines
- Supports cell growth and production of recombinant proteins and antibodies
- Contains a medium level of nutrients and growth factors
- Can be used in research or in manufacturing applications
- All steps, including thawing, expanding, pre-complexing DNA, transfecting and producing can be performed in the same medium regardless of the production scale
HEK TF
A Well-Established Transfection Medium Designed for HEK293 Cell Lines in Suspension Mode
- Developed for cultivating HEK293 cell lines, with a focus on transfection applications and virus production in suspension, making it ideal for gene therapy applications
- Well suited for transient transfection with polycationic transfection reagents
- Contains a medium level of nutrients and growth factors
- Can be used in research or in manufacturing applications
- All steps including thawing, expanding, pre-complexing DNA, transfecting and producing can be performed in the same medium
HEK ViP NB
Leanest Transfection Medium for HEK293 Cell Line in Suspension
- Developed for cultivation of HEK293 cell lines, with a focus on transfection applications and virus production in suspension — a perfect choice for gene therapy applications
- Well suited for transient transfection with polycationic transfection reagents
- Contains essential levels of nutrients and no growth factors
- Used in research or manufacturing applications
- All steps — thawing, expanding, pre-complexing DNA, transfecting and producing — can be performed in the same medium
HEK ViP NX
Highest Nutrient Level for HEK293 Cell Lines in Suspension
- Developed for cultivation of HEK293 and other mammalian cell lines, with a focus on transfection applications and optimized for virus/viral vector production, making it a perfect choice for gene therapy applications
- Contains high levels of nutrients and no growth factors
- Can be used in research or in manufacturing applications
- All steps — thawing, expanding, pre-complexing DNA, transfecting and producing — can be performed in the same medium regardless of the production scale
HEK FS
Unique to the Market - Concentrated Nutrients for HEK Cell Production
HEK-FS_2 is designed as a feeding solution in combination with Sartorius HEK293 media. The feed contains highly concentrated nutrients to increase the productivity of HEK293 and other human cells.
Chemically defined, animal component-free cell culture supplement
Developed to feed HEK media portfolio
Contains no L-glutamine, serum, hydrolysates or growth factors
Supports superior production of AAV or LV in suspension cultures by maintaining and extending the production ability of HEK cultures
To be used in a pH-controlled environment, must be added 6 – 24 h after transfection, at a minimum.
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FAQs - HEK 293 Media
Viruses are biological entities that replicate only within living cells. Most viruses can be grown in cultured cells such as MDCK, Vero, HEK, or insect cells. Historically embryonated hen's eggs were used to produce viruses before the industry moved to cell culture models.
The cell culture medium used to culture a virus greatly depends on the requirements of the host cell line. Specialty media provide the optimum quantity of nutrients to allow for cell growth and support virus infection and production. Among the human cell lines, HEK293 is widely utilized in viral vector production due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture.
HEK293 cells are easy to grow and maintain in culture. They are also highly amenable to transfection, delivering excellent transfection efficiencies. Thus, HEK293 cells are a preferred cell line for transfection experiments to produce gene therapies or gene-modified cell therapies.
Specialty media designed for HEK293 cells provides the optimal quantities of necessary nutrients to allow maximum cell growth, enable transfection, and support the production of high viral titers. Ideally, cells should be cultured in chemically-defined, serum-free media to limit variability between batches and simplify regulatory approvals.
Related Assets
The Tested Protocol to Scale Up AAV Production With HEK293 Host Cells
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Alluvial Filtration for Efficient Clarification of Suspension HEK293 Process Harvest in AAV Production
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