Specialty Media for HEK293 Cell Lines

HEK293 Media & Feed Portfolio

High-Performing Specialty Media and Feed for HEK293 Cell Lines

Growth in gene therapies and viral vector vaccines has increased the demand for robust HEK293 cell lines. Sartorius offers four high-performing suspension HEK media that are designed for use with the high variability of HEK293 cell lines, the versatility of adeno-associated virus and the complexity of viral vector processes. The portfolio also includes a unique feed supplement to boost viral production for gene therapies and vaccines.

Unique HEK Media for Your Unique Cells

HEK Media & Feed Portfolio for Optimized Growth

  • High performance, scalability and quality, ensuring purity and safety of the final product
  • Optimized for transient transfection and infection or stable expression in suspension
  • 100% chemically defined, animal component-free, serum-free
  • Available in liquid and powder formats
  • Requires little or no adaptation from other serum-free media
  • Manufactured under ISO 9001 & ISO 13485 quality standards

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Free HEK293 Media Sample Kit

Sample media for gene therapy and viral vector vaccines

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Robust and Effective Base Medium for Infection and Stable Expression

  • Developed for high-performance infection of HEK293 and other human cell lines
  • Supports cell growth and production of recombinant proteins and antibodies
  • Contains a medium level of nutrients and growth factors
  • Can be used in research or in manufacturing applications
  • All steps, including thawing, expanding, pre-complexing DNA, transfecting and producing can be performed in the same medium regardless of the production scale

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A Well-Established Transfection Medium Designed for HEK293 Cell Lines in Suspension Mode

  • Developed for cultivating HEK293 cell lines, with a focus on transfection applications and virus production in suspension, making it ideal for gene therapy applications
  • Well suited for transient transfection with polycationic transfection reagents
  • Contains a medium level of nutrients and growth factors
  • Can be used in research or in manufacturing applications
  • All steps including thawing, expanding, pre-complexing DNA, transfecting and producing can be performed in the same medium

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Leanest Transfection Medium for HEK293 Cell Line in Suspension

  • Developed for cultivation of HEK293 cell lines, with a focus on transfection applications and virus production in suspension — a perfect choice for gene therapy applications
  • Well suited for transient transfection with polycationic transfection reagents
  • Contains essential levels of nutrients and no growth factors
  • Used in research or manufacturing applications
  • All steps — thawing, expanding, pre-complexing DNA, transfecting and producing — can be performed in the same medium

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Highest Nutrient Level for HEK293 Cell Lines in Suspension

  • Developed for cultivation of HEK293 and other mammalian cell lines, with a focus on transfection applications and optimized for virus/viral vector production, making it a perfect choice for gene therapy applications
  • Contains high levels of nutrients and no growth factors
  • Can be used in research or in manufacturing applications
  • All steps — thawing, expanding, pre-complexing DNA, transfecting and producing — can be performed in the same medium regardless of the production scale

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Unique to the Market — Concentrated Nutrients for HEK Cell Production

HEK FS contains highly concentrated nutrients to increase the productivity of HEK293 and other human cells

  • Chemically defined, animal component-free cell culture supplement
  • Developed to feed HEK media portfolio
  • Contains no L-glutamine, serum, hydrolysates, lipids or growth factors
  • Supports superior production of recombinant proteins and antibodies in suspension culture by maintaining and extending the production ability of HEK cultures

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Featured Assets

eBook

A Comprehensive Guide to Finding the Right Cell Culture Media

In this eBook, our experts reveal valuable insights to help you develop an optimal cell culture media strategy for your bioprocess.

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Application Note

Adaptation of Mammalian Cell Lines to Serum-Free Suspension Culture

PDF 315.3 KB
Application Note

The Tested Protocol to Scale Up AAV Production With HEK293 Host Cells

Efficient AAV production starts with scalable upstream manufacturing. Learn how to scale up AAV production using HEK293 host cells.

Download Application Note

Request the HEK Media Sample Kit

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Complete Your Process With a Comprehensive Upstream Equipment Suite

Bioreactors & Fermenters

Sartorius offers a complete scalable bioreactor platform from cell and strain line development to commercial manufacturing.

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Liquid Storage & Shipping

Secure your product’s stability and integrity during long-term storage and shipping

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BLI Systems

Perform label-free, rapid viral quantitation for gene therapy applications.

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FAQs - HEK 293 Media

Viruses are biological entities that replicate only within living cells. Most viruses can be grown in cultured cells such as MDCK, Vero, HEK, or insect cells. Historically embryonated hen's eggs were used to produce viruses before the industry moved to cell culture models.

The cell culture medium used to culture a virus greatly depends on the requirements of the host cell line. Specialty media provide the optimum quantity of nutrients to allow for cell growth and support virus infection and production. Among the human cell lines, HEK293 is widely utilized in viral vector production due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture.

HEK293 cells are easy to grow and maintain in culture. They are also highly amenable to transfection, delivering excellent transfection efficiencies. Thus, HEK293 cells are a preferred cell line for transfection experiments to produce gene therapies or gene-modified cell therapies.

Specialty media designed for HEK293 cells provides the optimal quantities of necessary nutrients to allow maximum cell growth, enable transfection, and support the production of high viral titers. Ideally, cells should be cultured in chemically-defined, serum-free media to limit variability between batches and simplify regulatory approvals.

Related Assets

Flyer

HEK293 Media Sample Kit

PDF | 347.4 KB

Consult Our Experts

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