Sartorius Cell Culture Reagents & Supplements

Optimize Cell Growth and Propagation in Animal Component-free or Xeno-free Conditions

Consistent cell growth is an important factor in meeting bioprocessing milestones and can be affected by many conditions. Sartorius reagents and supplements can optimize your cell line’s growth. Every cell culture system is designed to meet its nutritional and metabolic niche requirements after a basal medium is chosen. To realize maximum yields, the cell culture must have a large reserve of both basic nutrients and the essential requirements to enhance cell growth and performance. Sartorius’ reagents are widely used in the field of cell and gene therapy as well as regenerative medicine.

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Growth Factors and Cytokines

High-performance growth factors and cytokines promote cell culture, expansion, and differentiation for cell-based therapies.

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Freezing Media and Solutions

High-quality freezing solutions designed and validated for the cryopreservation of cells including stem cells, T cells and PBMCs.

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Cell Culture Reagents and Supplements — Complete Portfolio From Sartorius

Support Cell Growth and Health With Sartorius Solutions

Sartorius offers a broad portfolio of high-quality cell growth supplements, dissociation solutions, attachment and matrix factors to support cell health, and cryopreservation solutions to protect cells during freezing and thawing.

A variety of Sartorius growth factors and cytokines are available to support ex vivo cell culture. These raw and ancillary materials are widely used in cell and gene therapy and regenerative medicine.

  • Xeno-free and ADCF
  • Purity ≥ 95%
  • Transferable to GMP

T Cells

Mesenchymal Stem Cells (MSC)

Pluripotent Stem Cells (PSC)

Hematopoietic Stem Cells (HSC)

Natural Killer Cells (NK Cells)

Dendritic Cells (DC)

Cytokines

IL-2 IL-7 IL-15 IL-21 TGF-β1

EGF
FGF-2
PDGF-BB
TGF-β1

EGF
FGF-2
Activin A
OSM
HGF
TGF-β1

SCF
TPO
Flt-3L
IL-3
IL-6

IL-2
IL-15
IL-21

GM-CSF
IL-4
IL-1β
TNF-a
IL-6
IL-10
IFN-γ

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NutriFreez® formulations are optimized to protect a wide range of human and animal-derived cells, including highly sensitive cell types such as primary cells, lymphocytes, and stem cells, in ultra-low temperatures of -196°C while assuring enhanced viability and recovery rates.

NutriFreez® D10

NutriFreez® D5

Formulation

Medium-based

Salt-based

DMSO
Concentration

10%

5%

Origin

Animal component-free

Animal component-free

Protein

Protein-free

Protein-free

Phenol Red

Optional

Without

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Sartorius offers a variety of attachment and matrix factors to facilitate the attachment, spreading, growth, morphology, differentiation, and motility of your cells.

NutriCoat™

MSC Attachment Solution

LaminStemTM

Vitronectin ACF

Cell type

MSC

MSC

PSC

PSC

Origin

Xeno-free

Xeno-free

Animal component-free

Animal component-free

Attachment protein

Fibrinogen

Fibronectin

Laminin

Vitronectin

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Recombinant Trypsin Solutions are animal component-free defined cell dissociation enzymes for the detachment of adherent cells.

Recombinant Trypsin Solution

Recombinant Trypsin EDTA Solution

Origin

Animal component-free

Animal component-free

EDTA

With

Without

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Sartorius offers PLTGold® Human Platelet Lysate, a rich growth factor supplement that is a superior alternative to FBS.

  • Available in research and clinical grades
  • Xeno-free
  • Heparin-free

PLTGold® Human Platelet Lysate (Research Grade)

PLTGold® Human Platelet Lysate (Clinical Grade)PLTGold® Human Platelet Lysate (Gamma Irradiated)

DMF

 

 

 

Application

Research

Clinical

Clinical

Gamma irradiated

No

No

Yes

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An effective supplement for maintaining human cell health, expansion and growth

Bio-Pure HSA

HSA

HSA Sterile

HSA
Concentration

10%

20%

20%

Origin

Xeno-free

Xeno-free

Xeno-free

Treated

Yes

Yes

No

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Promoting and Protecting Cell Health

Growth Factors and Cytokines

Growth factors and cytokines are widely used in cell and gene therapy for immune and stem cell applications, including activation, expansion, and differentiation.

  • High lot-to-lot consistency — save time and costs on revalidations
  • Performance reliability
  • High purity to enhance the safety of your ATMP
  • Extremely low endotoxin levels improve reproducibility

Browse Cytokines by Cell Type

NutriFreez® Cryopreservation Solutions

  • Optimized to protect a wide range of human and animal-derived cell types such as primary cells, lymphocytes and stem cells 
  • Enhanced cell viability and recovery
  • Animal component-free formulations
  • Widely referenced
  • cGMP Manufactured
  • Suitable for clinical applications
  • DMF available

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Matrices and Substrates | Attachment Solutions

Sartorius offers a variety of attachment and matrix factors to facilitate the attachment, spreading, growth, morphology, differentiation, and motility of your cells in a controlled xeno-free or animal component-free environment.

  • Specialty, defined solutions for human mesenchymal and pluripotent stem cells
  • Promote cellular survival and expansion
  • Easy to use
  • cGMP manufactured

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Cell Dissociation Solutions

Sartorius offers recombinant trypsin solutions for detaching adherent cells from a culture surface.

  • Replaces porcine trypsin and avoids animal-related variability and contamination
  • No protocol changes necessary
  • Optimized for sensitive cell types, a critical characteristic in preventing harsh effects for downstream processing
  • Defined, animal component-free
  • Ready-to-use
  • cGMP manufactured

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Quality Cell Culture Supplements

PLTGold® Human Platelet Lysate

Human platelet lysate is an effective, high-protein supplement for human cell culture and expansion used to minimize the risk of xenogenic immune reactions or prion/zoonotic disease transmission.

  • Has all the growth factors and proteins needed for cell growth
  • Xeno-free, animal serum-free
  • Supports excellent cell growth and proliferation
  • Controlled lot-to-lot variability
  • DMF available

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Human Serum Albumin (HSA)

Human serum albumin is a multifunctional agent with various biological properties. Sartorius offers raw HSA as a supplement for cell cultures, as well as specially treated HSA for human stem cell culture in 10% and 20% concentrations

  • Xeno-free
  • Supports cell health and expansion
  • Maintains cell membrane stability

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Learn More About Cell Culture Reagents

Related Topics

T Cell & Immunotherapy Cell Culture Media

Xeno-free, serum-free media for cultivating various types of immune cells and immunotherapy applications.

Explore 4Cell® Nutri-T Media

Stem Cell Media

Unique stem cell media and reagents promote cell maintenance and cell health, allowing smooth transfers to clinical applications.

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Cell Cryopreservation Solutions

High-quality freezing solutions designed and validated for the cryopreservation of cells including stem cells, T cells, and PBMCs.

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Complete Your Application

Stem Cell Culture Media and Reagents

High-quality serum-free stem cell culture media to support the reproducible generations of quality-assured and clinically acceptable stem cells.

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Cell Booster Bundle

Build your ideal bundle for cell culture with our selection of media, growth factors, cytokines, and microcarriers.

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Ultroser™ Serum Substitute

Ultroser™ G can successfully replace FCS (fetal calf serum) in cell culture medium. It is designed for the in vitro culture of anchorage-dependent cel...

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Related Assets

Application Note

Single Cell Passaging With NutriStem® hPSC XF on Laminin-521 Without the Need for ROCK Inhibitor

PDF | 1.8 MB
Application Note

Passaging MSC Using NutriStem® XF Medium and Human Platelet Lysate

PDF | 230.1 KB

Consult Our Experts

Consult Our Experts

Related Topics

T Cell & Immunotherapy Cell Culture Media

Xeno-free, serum-free media for cultivating various types of immune cells and immunotherapy applications.

Explore 4Cell® Nutri-T Media

Stem Cell Media

Unique stem cell media and reagents promote cell maintenance and cell health, allowing smooth transfers to clinical applications.

Learn More

Cell Cryopreservation Solutions

High-quality freezing solutions designed and validated for the cryopreservation of cells including stem cells, T cells, and PBMCs.

Learn More