Classical Media Manufactured to Meet the Highest Quality Standards

Sartorius media are designed to support the growth and maintenance of a variety of cells and cell lines. Each lot is manufactured under a strictly controlled process according to a Product Master Record to provide lot-to-lot consistency. Sartorius offers ready-to-use media in liquid formulations with various packaging options. Sartorius‘ excellence and expertise provide you with the safest, most reliable and most consistent media products.

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Ultroser® G Serum Substitute Replaces Fetal Calf Serum (FCS)

Ultroser® G, a semi-defined serum substitute for FCS, is designed for the in vitro culture of anchorage-dependent cells.

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Classical Media From Sartorius

Find the Right Classical Cell Culture Medium for Your Application

Classical media are produced using well-known formulations, following cGMP guidelines, and manufactured with the highest-grade raw materials, sourced from qualified and approved suppliers.

  • Ready-to-use media in concentrated liquid formulations
  • Varied packaging options



RPMI 1640


Liquid 100ml – 500ml

Liquid 100ml – 500ml

Liquid 100ml – 500ml


Available in different options:

  • 1X, 2X and 5X
  • High/low glucose
  • With or without glutamine

Available in different options:

  • 1X and 10X
  • With or without sodium bicarbonate

Available in different options:

  • With or without phenol red
  • With or without glutamine
  • With or without glucose
  • With or without sodium bicarbonate or HEPES

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Dulbecco's Modified Eagle Medium (DMEM)

DMEM is a widely used basal medium for supporting the growth of many mammalian cells, including primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells. Sartorius offers a variety of DMEM modifications for a range of cell culture applications.

  • Available in high and low glucose formulations

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MEM-Eagle — A Modified Minimum Essential Medium (MEM)

MEM is one of the most used cell culture media and can be used with a variety of suspension and adherent mammalian cells, including HeLa, fibroblasts and primary rat astrocytes. Sartorius offers a variety of MEM modifications for a wide range of cell culture applications.

  • Available with both Hank’s and Earle’s balanced salt solutions for mammalian cells

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Roswell Park Memorial Institute (RPMI) 1640 Medium

RPMI 1640 Medium was specifically developed for the long-term culture of blood cells and the culture of normal and abnormal human leukocytes.

  • Used as a general medium (with serum) for hybridoma cultures
  • Suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas
  • Modifications available for various applications
  • Available in multiple formulations

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Additional Classical Media

Use the following multi-purpose cell culture media alone or with other classical media formulations:

  • Basal Medium Eagle
  • Ham’s F10 Nutrient Solution
  • Ham’s F12 Nutrient Solution
  • Minimum Essential Medium Alpha
  • MCDB 153 (modified)
  • Iscove’s Modified Dulbecco’s Medium
  • Waymouth's MB 752/1 Medium

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Classical Media Manufactured Following cGMP Guidelines Under ISO:9001

All products are manufactured under the ISO 9001 and 13485 Quality Management Systems (QMS) and in compliance with applicable cGMP guidelines.

FAQs - Classical Media

Classical media are the first generation of cell culture media and have been used in cell culture research for decades. They are a mixture of inorganic salts, amino acids, sugars, vitamins, and other components needed for optimal growth. They are typically used with serum and are multi-purpose, meaning they can be used to grow different cell lines.

Complete Your Process With Comprehensive Solutions

Incucyte® Live Cell Analysis uses whole-well, label-free imaging to scan clones and verify monoclonality.

Live Cell Imaging & Analysis

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scientist with pipette

Mycoplasma Detection

Explore Mycoplasma Detection

Cell Health & Proliferation

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Mycap® Single-Use Aseptic Transfer System for Bottles

One-piece, ready-to-use, and single-use aseptic fluid transfer system for bottles.

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Customized Media and Buffers

Robust and reliable production of customized cell culture media and buffers based on existing formulations or improvement of recipes.

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