Stem Cell Media

Media and Reagents for Research, Clinical and Commercial Use

The development of cell-based therapies is driving demand for high-quality stem cell media. Sartorius offers a full range of xeno-free and animal component-free stem cell media, including cell culture media, stem cell freezing solutions, stem cell differentiation media, attachment factors and cell dissociation solutions. Sartorius’ media meet superior quality standards, are extensively QC tested and functionally characterized to perform in relevant pluripotent or multipotent stem cells. 

Stem Cell Media Portfolio

Find the Right Stem Cell Media and Reagent for Your Application

Sartorius‘ portfolio of stem cell culture media, reagents and freezing solutions provides support for culturing, reprogramming, characterizing, and differentiating stem cells. From identifying colonies and their lineages to monitoring changing morphology, these stem cell media products accelerate scientists’ progress in understanding disease processes and developing advanced stem cell therapies.


hPSC Culture

hMSC Culture

hHSPC Culture

Media

NutriStem® hPSC XF Culture Media

MSC NutriStem® XF Culture Media

CellGenix® GMP SCGM ​

Attachment

LaminStem™/Vitronectin

NutriCoat™/MSC Attachment Solution

 

Dissociation

Recombinant Trypsin Solution

Recombinant Trypsin Solution

 

Cryopreservation

NutriFreez® Cryopreservation

NutriFreez® Cryopreservation

NutriFreez® Cryopreservation

Differentiation

NutriStem® hPSC GF-free

MSCgo™ Differentiation Media

 

Supplement

Human Serum Albumin

PLTGold® Platelet Lysate

 

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Human Pluripotent Stem Cell (hPSC) Culture

hPSC culture conditions significantly impact cellular characteristics, growth, and potential downstream use. For therapeutic stem cell applications, cultures must be scalable and free of xenogenic factors. NutriStem® hPSC media are derived from defined components and xeno-free.

  • Support long-term culture of hPSCs
  • Preserve pluripotency and hPSC normal karyotypes
  • cGMP manufactured
  • DMF available

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Human Mesenchymal Stem/Stromal Cell (hMSC) Culture

hMSCs are promising tools for regenerative medicine and cellular therapies. When expanding hMSCs in vitro for translational use, a cGMP-compliant culture that generates large numbers of healthy, proliferative hMSCs is vital. MSC NutriStem® is xeno-free and serum-free.

  • Exceptional hMSC proliferation and rapid expansion
  • Used in multiple clinical trials worldwide
  • cGMP manufactured
  • DMF available

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Mesenchymal Stem Cell Differentiation

Stem cell differentiation for research, drug discovery or therapeutic applications must be reproducible and reliable. MSCgo™ Differentiation Media are serum-free and xeno-free for the differentiation of osteogenic, adipogenic, and chondrogenic lineages.

  • Ensure hMSC culture integrity
  • Complete and ready-to-use
  • Optimized differentiation protocols
  • Suitable for various sources of hMSCs

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Stem Cell Cryopreservation

Freezing stem cells while minimizing changes in cellular genetics is vital to reserving cells for future clinical and experimental use. NutriFreez® optimized freezing solutions are designed and validated for use with many cell types including hESCs, iPSCs, and hMSCs.

  • Chemically defined, animal component-free, protein-free
  • High recovery and viability post-thaw
  • cGMP manufactured
  • DMF available

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Human Hematopoietic Stem and Progenitor Cell (hHSPC) Culture

hHSPCs are used for autologous and allogeneic cell therapies. CellGenix® GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers of human hematopoietic stem and progenitor cells.​

  • ​Serum-free cultivation of HSCs​
  • Expansion and differentiation of NK cells and CIK cells​
  • GMP manufactured​
  • FDA & PMDA DMF available

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Facilitating Stem Cell Research and Advanced Cell-Based Therapies

Why Use Sartorius’ Stem Cell Media?

Sartorius’ innovative and reliable stem cell media and reagents enable high-quality cell maintenance and cell health, allowing smooth transfers to clinical applications.

  • Defined, serum-free, xeno-free culture systems for hMSCs or hiPSCs and hESCs 
  • Superior proliferation rate 
  • Supporting long-term growth and differentiation
  • Specially developed animal component-free solutions for attachment, dissociation, cryopreservation, and differentiation
  • Complete protocols, application data and scientific references available
  • FDA Drug Master Files (DMF) available
  • Manufactured in accordance with ISO 13408 and cGMP guidelines

FAQs - Stem Cell Media

Stem cells can differentiate to several cell types:

  1. Pluripotency stem cells are from embryonic cells or targeted cells that can be reprogrammed to iPSC. Both cell types have self-renewal properties, and can stay undifferentiated for at least 50 passages.
    They can differentiate to each cell from the three lineages of the embryonic germ layer — endoderm, mesoderm, and ectoderm, and  can be used to regenerate tissue in practice.
    For these cells, scientists need a serum-free medium with controlled levels of growth factors to keep cells undifferentiated for as long as possible. As the serum is eliminated from the medium, scientists must add an attachment matrix or carrier (animal source or defined) to the culture to allow the cells to expand.
  2. MSCs are multipotency cells from connective tissues of the body. They have the ability to stay undifferentiated for up to six passages, and can spontaneous differentiate to one of three cell types — chondrocytes, osteocytes, and adipocytes. The cells can be differentiated to other cell types by using specific growth factors. The media we offer keeps cells undifferentiated for at least six passages (depending on the cell source) and allows fast differentiation when needed. As serum is eliminated from the medium, you can add attachment factors or microcarriers.

Sartorius offers media for pluripotent stem cells (PSCs) and mesenchymal stromal cells (MSCs), as well as ancillary products for the culture process from isolation to expansion and differentiation finished with cryopreserved cells.

Media can keep PSCs undifferentiated for at least 50 passages and MSCs for at least 6 passages.

  • PSCs: One ready-to-use bottle of NutriStem® hPSC XF Media.
  • MSCs: Basal media with supplements. As the media is serum-free, it needs to be supplemented with attachment matrix. You can supplement PSCs with laminine and vitronectine, and MSCs with fibrinogen and fibronectin.
  • Detachment: A peptide of recombinant trypsin with or without EDTA.
  • Differentiation: Kits for MSC to chondrocytes, osteocytes and adipocytes.
  • Cryopreservation: Medium based with 10% DMSO and salt based with 5% DMSO.

Once you have fully characterized   the growth properties of your cells, a tailored media formulation can help optimize their growth and productivity.

You can get customized media formulations that add or remove elements, or create a complete custom cell culture media formulation to meet your specifications.

Totipotent stem cells: Totipotent (omnipotent) stem cells can differentiate into any of the 220 cell types found in an embryo as well as extra-embryonic cells (placenta).
Pluripotent stem cells: Pluripotent stem cells can differentiate into all cell types of the body (but not the placenta). Embryonic stem cells and induced pluripotent cells are under this potency.
Multipotent stem cells: Multipotent stem cells can develop into a limited number of cell types in a particular lineage. MSCs are under this potency.

Complete Your Process With Comprehensive Solutions

Cell Booster Bundle Makes Finding the Right Media Easy

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Mycoplasma Detection

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SX5 Live Cell Analysis Instrument

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Microcarriers

Six types of SoloHill® animal protein-coated and animal componentfree microcarriers are available to support adherent cell culture growth.

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Univessel® Glass

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Cytokines for Immune Cell Culture

Target immune cell growth with different combinations of cytokines such as IL-2, IL-7, IL-15 and IL-21

Explore Cytokines

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Media Services & Custom Manufacturing - Accelerating the Evolution of Cell Culture Manufacturing

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Optimization of a Scalable Single-Use Manufacturing Platform

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Consult Our Experts

Consult Our Experts

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