Xeno-Free and Serum-Free Stem Cell Media for Regenerative Medicine
The development of cell-based therapies is driving demand for high-quality stem cell media. Sartorius offers a full range of xeno-free and serum-free stem cell media, including cell culture media, stem cell differentiation media, attachment factors and cell dissociation solutions. Sartorius’ media meet superior quality standards, are extensively QC tested and functionally characterized to perform in relevant pluripotent or multipotent stem cells.
Xeno-Free, Serum-Free Stem Cell Media Portfolio
Find the Right Stem Cell Media and Reagent for Your Application
Sartorius‘ portfolio of stem cell culture media, reagents and freezing solutions provides support for culturing, reprogramming, characterizing, and differentiating stem cells. From identifying colonies and their lineages to monitoring changing morphology, these stem cell media products accelerate scientists’ progress in understanding disease processes and developing advanced stem cell therapies.
| PSC Culture | MSC Culture | HSPC Culture | |
|---|---|---|---|
| Media | NutriStem® hPSC XF Culture Media | MSC NutriStem® XF Culture Media | CellGenix® GMP SCGM |
| Attachment | — | NutriCoat™ | — |
| Dissociation | Recombinant Trypsin Solution | Recombinant Trypsin Solution | — |
| Cryopreservation | NutriFreez® Cryopreservation | NutriFreez® Cryopreservation | NutriFreez® Cryopreservation |
| Differentiation | NutriStem® hPSC GF-free | MSCgo™ Differentiation Media | — |
| Supplement | — | PLTGold® Platelet Lysate | — |
| View Products | View Products | View Products |
Human Pluripotent Stem Cell (hPSC) Culture
hPSC culture conditions significantly impact cellular characteristics, growth, and potential downstream use. For therapeutic stem cell applications, cultures must be scalable and free of xenogenic factors. NutriStem® hPSC media are derived from defined components and xeno-free.
- Support long-term culture of hPSCs
- Preserve pluripotency and hPSC normal karyotypes
Manufactured in accordance with applicable cGMP guidelines
- DMF available
Human Mesenchymal Stem/Stromal Cell (hMSC) Culture
hMSCs are promising tools for regenerative medicine and cellular therapies. When expanding hMSCs in vitro for translational use, a cGMP-compliant culture that generates large numbers of healthy, proliferative hMSCs is vital. MSC NutriStem® is xeno-free and serum-free.
- Exceptional hMSC proliferation and rapid expansion
- Used in multiple clinical trials worldwide
- Manufactured in accordance with applicable cGMP guidelines
- DMF available
Human Hematopoietic Stem and Progenitor Cell (hHSPC) Culture
hHSPCs are used for autologous and allogeneic cell therapies. CellGenix® GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers of human hematopoietic stem and progenitor cells.
- Serum-free cultivation of HSCs
- Expansion and differentiation of NK cells and CIK cells
- GMP manufactured
- FDA & PMDA DMF available
Cell Culture Supplements
PLT Supplement for Human Mesenchymal Stem/Stromal Cell Culture
PLTGold® Human Platelet Lysate is a xeno-free, animal serum-free product derived from human platelets.
Contains all the growth factors and proteins necessary for hMSC growth
Available in research and clinical grades
Manufactured in a cGMP-compliant facility
Does not require the addition of heparin
Drug Master File (DMF) available
Optimal Adherence of Human Mesenchymal Stem/Stromal Cells
Attachment factors are critical for adherence of hMSCs, particularly when grown in serum-free culture.
Xeno-free attachment solution
Facilitate attachment, cell spreading, growth, morphology, differentiation, and motility of hMSCs
Manufactured in accordance with applicable cGMP guidelines
Reliable Recombinant Trypsin for hMSC Dissociation
Recombinant Trypsin Solutions are animal component-free, defined cell dissociation enzymes that replace porcine trypsin and avoid animal-related variability and contamination.
Ideal for dissociating attachment-dependent cells, such as hMSCs, in both serum-containing and serum-free conditions
Can be directly substituted for trypsin without protocol changes
Optimized for sensitive cell types, a critical characteristic in preventing harsh effects for downstream processing
Efficient Differentiation of Human Mesenchymal Stem Cells
Whether for basic research, drug discovery, or therapeutic applications, stem cell differentiation requires standardized culture methods to ensure reproducible and reliable results. Sartorius offers a unique line of MSCgo™️ differentiation media.
Serum-free and xeno-free
Effectively differentiate hMSCs into adipocytes, chondrocytes, and osteoblasts
Suitable for hMSC from various sources (hMSC-AT, hMSC-BM, and hMSC-CT)
Contains all the growth factors and supplements
Test a Stem Cell Media Sample
Sample media for stem cell-based therapies.
Complete Your Process With Comprehensive Solutions
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FAQs - Stem Cell Media
Stem cells can differentiate to several cell types:
- Pluripotency stem cells are from embryonic cells or targeted cells that can be reprogrammed to iPSC. Both cell types have self-renewal properties, and can stay undifferentiated for at least 50 passages.
They can differentiate to each cell from the three lineages of the embryonic germ layer — endoderm, mesoderm, and ectoderm, and can be used to regenerate tissue in practice.
For these cells, scientists need a serum-free medium with controlled levels of growth factors to keep cells undifferentiated for as long as possible. As the serum is eliminated from the medium, scientists must add an attachment matrix or carrier (animal source or defined) to the culture to allow the cells to expand. - MSCs are multipotency cells from connective tissues of the body. They have the ability to stay undifferentiated for up to six passages, and can spontaneous differentiate to one of three cell types — chondrocytes, osteocytes, and adipocytes. The cells can be differentiated to other cell types by using specific growth factors. The media we offer keeps cells undifferentiated for at least six passages (depending on the cell source) and allows fast differentiation when needed. As serum is eliminated from the medium, you can add attachment factors or microcarriers.
Sartorius offers media for pluripotent stem cells (PSCs) and mesenchymal stromal cells (MSCs), as well as ancillary products for the culture process from isolation to expansion and differentiation finished with cryopreserved cells.
Media can keep PSCs undifferentiated for at least 50 passages and MSCs for at least 6 passages.
- PSCs: One ready-to-use bottle of NutriStem® hPSC XF Media.
- MSCs: Basal media with supplements. As the media is serum-free, it needs to be supplemented with attachment matrix. You can supplement PSCs with laminine and vitronectine, and MSCs with fibrinogen and fibronectin.
- Detachment: A peptide of recombinant trypsin with or without EDTA.
- Differentiation: Kits for MSC to chondrocytes, osteocytes and adipocytes.
- Cryopreservation: Medium based with 10% DMSO and salt based with 5% DMSO.
Once you have fully characterized the growth properties of your cells, a tailored media formulation can help optimize their growth and productivity.
You can get customized media formulations that add or remove elements, or create a complete custom cell culture media formulation to meet your specifications.
Totipotent stem cells: Totipotent (omnipotent) stem cells can differentiate into any of the 220 cell types found in an embryo as well as extra-embryonic cells (placenta).
Pluripotent stem cells: Pluripotent stem cells can differentiate into all cell types of the body (but not the placenta). Embryonic stem cells and induced pluripotent cells are under this potency.
Multipotent stem cells: Multipotent stem cells can develop into a limited number of cell types in a particular lineage. MSCs are under this potency.