T Cell and Immunotherapy Media

Cell Activation and Expansion for Immunotherapy and Immunology Research

Elucidating the complex cellular interactions involved in immune responses is critical to developing novel and effective immunotherapies. This requires effective and consistent tools to standardize immunology research. Sartorius offers high-quality media, developed and optimized specifically for culturing PBMCs, T cells, CAR-Ts and TILs. With the serum-free, xeno-free 4Cell® Nutri-T Media, you can simplify your process, reduce the risks associated with serum, and ease your regulatory procedures.

Robust T Cell and Immunotherapy Culture

Cell-Based Immunotherapy Culture

  • Serum-free, xeno-free media
  • Optimized for T cell, TIL, CAR-T, Treg and PBMC applications
  • Excellent performance without the addition of human serum
  • Manufactured in accordance with applicable cGMP guidelines
  • Seamless transition from research to the clinic
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Test a T Cell Media Sample

Sample media for cell-based immunotherapy.

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Meet the Media – Our Series on GMP Grade Media

In this series, we talk to our media experts about the challenges our customers face in finding suitable media, feeds, and raw materials for their cell culture projects. 


4Cell® NutriT GMP Medium | Meet the Media Episode 1

In this episode, we talk to our media experts about the challenges in growing and activating T cells and how to overcome these challenges.

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CellGenix® GMP TCM | Meet the Media Episode 2

In this episode, we talk to our media experts about the applications and flexibility of this T-cell media.

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Media Purchasing Made Easy

Select your media type and benefit from our simplified shopping experience.

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Culture Medium for T cells, CAR-Ts, TILs, and PBMCs for Clinical Application

Serum-free, xeno-free, ready-to-use medium provides excellent performance with both healthy and patient-derived donor cells.

  • Drug Master File is available
  • Manufactured in accordance with ISO 13485 and with applicable cGMP guidelines
  • Regulatory and scientific consultation and assistance
  • Scale-up and customization capabilities

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CellGenix® GMP TCM

Culture Medium for T cells, CAR-Ts and Tregs

Serum-free and xeno-free medium provides exceptional performance with no need to add serum.​

  • Promotes an early-differentiated phenotype​

  • Manufactured in accordance with USP Chapter <1043>, ISO 20399:2022 and all applicable GMP guidelines​

  • FDA Drug Master File (DMF) coming soon

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Ideal for the Activation and Expansion of T Cells, NK and CIK Cells

  • Animal derived component-free (ADCF)
  • Provides high cell yield and desired phenotypes
  • Manufactured in accordance with cGMP guidelines
  • Preclinical and GMP versions available
  • High purity and extremely low endotoxin levels

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Cell Culture Technology

Webinar | Optimizing Perfusion Parameters for Intensified Production o...

Discover methods for growing and enhancing CAR-T cells in automated stirred-bioreactors. Use the best perfusion parameters for optimal results.

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Featured Assets


Expand Lymphocytes with 4Cell® Nutri-T Xeno- and Serum-Free Medium

4Cell® Nutri-T: Learn the benefits of a xeno- and serum-free medium for process control and selective expansion of cell subpopulations.

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CellGenix® GMP TCM and CellGenix® Cytokines in CAR-T Processes

Find out how CellGenix® GMP TCM cell culture media and CellGenix® Cytokines can be used to optimize CAR-T cell manufacturing. 

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Improved Car-T Cell Expansion in Ambr® 250 High-Throughput Bioreactor

Learn how optimizing a perfusion process with quality by design impacts CAR-T cell growth and phenotype.

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Excellent Performance and Flexibility

High Cytotoxic Activity

4Cell® Nutri-T medium promotes higher IFN-gamma secretion post-co-culturing with autologous patient’s melanoma cells compared to competitor medium + 5% human serum

High Expansion Rate of PBMCs

4Cell® Nutri-T medium supports a higher expansion rate of healthy PBMCs compared to competitor media

High Expansion Rate of CAR-T Cells

4Cell® Nutri-T medium supports a higher expansion rate of CAR-T cells compared to competitor media

High Percentage of CD8+ Cells

4Cell® Nutri-T medium exhibits a high percentage of CD8+ cells in patient-derived cancer cells for TIL processes

Learn More about 4Cell® Nutri-T Medium

Sartorius’ Cell Culture Media for Your Cell-Based Immunotherapy 

4Cell® Nutri-T is the ideal culture medium for the development and scale-up of T-cell-based therapeutic immunotherapy and cell therapy applications. It is the only medium that does not require the addition of serum, hence simplifying the process, and is more compliant with regulatory requirements.

  • Quickly generate high cell yields
  • Increase therapeutic potential
  • Enable seamless clinical transitions
  • Trust lot-to-lot consistency and extensive documentation

FAQ - T-Cell & Immunotherapy Media

  1. Sample preparation: Using leukapheresis to prepare and concentrate whole blood components (e.g., patient’s PBMCs separated from red blood cells).                                                                                        
  2. Cell activation: Enriching, selecting, and activating T-Cells using anti-CD3 and anti-CD28 coated antibodies (typically bead-based) and growth factors (e.g., IL-2).                                                 
  3. Transduction: Genetically modifying endogenous T-Cells to express a specific receptor. After, the excess vector and other residuals are washed from the culture.                       
  4. Expansion: Expanding T-Cells initially in static conditions, and then performing further expansions using static means or GMP agitation bioreactor methods. Scientists continue expansion until enough cells reach clinical dose requirements. During the expansion stage, volume grows based on the number of cells. Both phases of expansion can also be done in an integrated closed system.                                                                
  5. Harvest: Harvesting engineered T-Cells using gravity settlement or counter-flow centrifugation. Scientists might use several wash steps before formulating the final media. The product is then transferred to an infusion medical device bag.
  6. Cryopreservation: Preserving cells in medium with 10% DMSO and salt with 5% DMSO and freezing for long-term storage.       

Complete Your Process With Comprehensive Solutions

Cytokines for Immune Cell Culture

Target immune cell growth with different combinations of cytokines such as IL-2, IL-7, IL-15 and IL-21

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Cell Biopreservation Solutions

High-quality solutions designed and validated for the cryopreservation and cold storage of cells, including stem cells, T cells, and PBMCs.

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Bioreactors & Fermenters

Sartorius offers a complete scalable bioreactor platform from cell and strain line development to commercial manufacturing.

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scientist with pipette

Mycoplasma Detection

Explore Mycoplasma Detection
iQue Advanced Flow Cytometry Platform

Flow Cytometry

Combine cell immuno-phenotyping, cell health, and cytokine analysis to quantitate immune cell function

Explore iQue® Cytometer
Incucyte® Live Cell Analysis uses whole-well, label-free imaging to scan clones and verify monoclonality.

Incucyte® SX5 System

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Related Assets

Application Note

CAR-T Expansion Using the Sartorius T-Cell Expansion Solution

PDF | 897.4 KB

Development of a Target Binding SPR Assay for Therapeutic Antibodies and Fc Fusion Proteins

PDF | 685.7 KB
User Guide

Cryopreservation Guide - The basics of cellular cryopreservation for research & clinical use

PDF | 3.0 MB

Consult Our Experts

Consult Our Experts

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