Immune Cell Therapy Media
Xeno-Free and Animal Component-Free Immune Cell Media for Immunotherapy and Immunology Research
Human serum and fetal bovine serum have traditionally been used for the expansion of immune cells. However, the associated batch-to-batch variability, risk of contamination by adventitious agents, sourcing challenges, and regulatory complexity pose significant hurdles for cell therapy developers striving to bring their therapies to the clinic. To address these challenges, our immune cell media solutions are designed to eliminate the variability and risks linked to serum-derived components—enabling greater consistency, safety, and regulatory compliance. Explore our xeno-free and animal component-free media portfolio, specifically developed and optimized for the culture of PBMCs, T cells, CAR-T cells, Tregs, TILs, NK cells, and dendritic cells.
Our Newest Animal Component-Free Cell Media Portfolio
Our animal component-free media formulation eliminates the variability and safety concerns associated with human serum, making it ideal for transition into clinical manufacturing.
Animal Component-Free Cell Culture Media for T cells, CAR-Ts, TILs, and PBMCs
Our two chemically defined, ready-to-use media (4Cell® Nutri-T GMP Advanced and CellGenix® GMP Advanced TCM) provide excellent performance with both healthy and patient-derived donor cells. Test both media today to find the best fit for your process and cells.
In-house production of critical raw material—recombinant human albumin (Recombumin®)
Regulatory and scientific consultation and assistance
Scale-up and customization capabilities
Research Use Only, GMP available soon
4Cell® Nutri-T GMP Advanced and CellGenix® GMP Advanced TCM are offered in convenient single-use bags of different sizes, designed to support GMP-compliant manufacturing environments. These bags are available with a choice of tubing materials—thermoplastic elastomer (TPE) or polyvinyl chloride (PVC)—to accommodate different process requirements and compatibility preferences in cell and gene therapy workflows.
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Excellent Expansion and Viability with Healthy Donor-derived T Cells
4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM support superior T cells expansion from healthy donor-derived CD3+ T cells compared to alternative chemically defined media after 10-day culture. Viability of T cells is maintained above 90%.
Long Term High Transduction Efficiency and Robust CAR Expression
4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM support stable CAR expression and high CAR-T cell yield compared to alternative chemically defined media after 10-day culture.
Advanced T Cell Media Support Growth of Both CD4+ and CD8+ T Cells
4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM support the growth of both CD4+ and CD8+ T cells after 10-day culture. Donor-specific variability in CD4/CD8 ratio is observed.
Stem Cell-like Memory Phenotypes were Well Maintained
Cells cultured in 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM display mainly undifferentiated phenotypes in both CD4+ and CD8+ T cells. In contrast, medium supplemented with serum increased the frequencies of effector memory T cells re-expressing CD45RA (Temra).
Highly Functional & Effective CD19 CAR-T Cells
CAR-T cells cultured for 7 days in our 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM are highly cytotoxic and specifically kill CD19-expressing NALM6 cells.
Robust Expansion and High Viability in Patient-derived CAR-T Cells
Patient-derived CAR-T cells expanded better with high viability in 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM.
High Transduction Efficiency with Patient-derived CAR-T Cells
Stable CAR-expression was maintained and high frequency of CD8 T cells was observed in patient-derived CAR-T cells after 10-day culture in 4Cell® Nutri-T Advanced Medium and CellGenix® Advanced TCM.
Xeno-Free Immune Cell Media Portfolio with Proven Performance
Our serum-free, xeno-free media portfolio is ideal for early-stage research and process development.
Culture Medium for T cells, CAR-Ts, TILs, and PBMCs for Clinical Application
Serum-free, xeno-free, ready-to-use medium provides excellent performance with both healthy and patient-derived donor cells.
- Drug Master File is available
- Manufactured in accordance with ISO 13485 and with applicable cGMP guidelines
- Regulatory and scientific consultation and assistance
- Scale-up and customization capabilities
Culture Medium for T cells, CAR-Ts and Tregs
Serum-free and xeno-free medium provides exceptional performance with no need to add serum.
Promotes an early-differentiated phenotype
Manufactured in accordance with USP Chapter <1043>, ISO 20399:2022 and all applicable GMP guidelines
FDA Drug Master File (DMF) is available
Culture Medium for Hematopoietic Stem and Progenitor Cells, Natural Killer Cells, and Human Cytokine Induced Killer Cells
- Optimized, xeno-free, serum-free medium
- Serum-free expansion of low numbers of isolated human hematopoietic stem and progenitor cells (HSCs/CD34+ cells)
- Highly efficient for the expansion and cultivation of human natural killer cells (NK cells) and human cytokine induced killer cells (CIK cells)
- Manufactured in accordance with applicable GMP guidelines
- FDA & PMDA Drug Master Files (DMFs) are available
- Outstanding QC support (e.g. extensive stability data)
- Technical and regulatory support
Culture Medium for Dendritic Cells
- Optimized, xeno-free, serum-free medium
- Engineered for the generation of human dendritic cells (DCs)
- Promotes high yields of mature dendritic cells with the desired phenotype
- Manufactured in accordance with applicable GMP guidelines
- FDA Drug Master File (DMF) is available
- Outstanding QC support (e.g. extensive stability data)
- Technical and regulatory support
Explore All Raw Material Samples For Immune Cells
Excellent Performance and Flexibility
High Cytotoxic Activity
4Cell® Nutri-T medium promotes higher IFN-gamma secretion post-co-culturing with autologous patient’s melanoma cells compared to competitor medium + 5% human serum.
High Expansion Rate of PBMCs
4Cell® Nutri-T medium supports a higher expansion rate of healthy PBMCs compared to competitor media.
High Expansion Rate of CAR-T Cells
4Cell® Nutri-T medium supports a higher expansion rate of CAR-T cells compared to competitor media.
High Percentage of CD8+ Cells
4Cell® Nutri-T medium exhibits a high percentage of CD8+ cells in patient-derived cancer cells for TIL processes.
High T Cell Expansion
CellGenix® GMP TCM supports greater T cell expansion than other serum-free competitor media while maintaining a high level of viability.
High Proportion of CD19 CAR-T Cells
Ex vivo expansion of lentivirus-transduced T cells results in a high proportion of CD19 CAR-T cells in CellGenix® GMP TCM.
High Killing Capacity
CAR-T cells generated with CellGenix® GMP TCM specifically target and kill CD19+ NALM-6 cells with low donor-dependent variance.
Related Assets
App Note
Optimal Combinations of CellGenix® Reagents Boost Natural Killer Cell Expansion, Viability, and Purity
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Articles
Webinars
Meet the Media – Our Series on GMP Grade Media
In this series, we talk to our media experts about the challenges our customers face in finding suitable media, feeds, and raw materials for their cell culture projects.
FAQ - T-Cell & Immunotherapy Media
- Sample preparation: Using leukapheresis to prepare and concentrate whole blood components (e.g., patient’s PBMCs separated from red blood cells).
- Cell activation: Enriching, selecting, and activating T-Cells using anti-CD3 and anti-CD28 coated antibodies (typically bead-based) and growth factors (e.g., IL-2).
- Transduction: Genetically modifying endogenous T-Cells to express a specific receptor. After, the excess vector and other residuals are washed from the culture.
- Expansion: Expanding T-Cells initially in static conditions, and then performing further expansions using static means or GMP agitation bioreactor methods. Scientists continue expansion until enough cells reach clinical dose requirements. During the expansion stage, volume grows based on the number of cells. Both phases of expansion can also be done in an integrated closed system.
- Harvest: Harvesting engineered T-Cells using gravity settlement or counter-flow centrifugation. Scientists might use several wash steps before formulating the final media. The product is then transferred to an infusion medical device bag.
- Cryopreservation: Preserving cells in medium with 10% DMSO and salt with 5% DMSO and freezing for long-term storage.
