High-throughput Screening Tools to Improve and Accelerate Your Cell Line Development

Innovative Technologies to Select the Right Clone

Sartorius provides innovative, high-throughput and automated solutions for early development of dedicated cell lines. Instruments to identify top-performing cell lines with the optimal critical quality attributes (CQAs) can not only achieve high yields of recombinant proteins, but also smooth the transition to commercial manufacturing. Eliminate process bottlenecks by investing in the most advanced technologies for cell line selection, characterization, and cell bank preparation.

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Make Better Predictions Earlier

Early prediction of the best-performing clone means you can get it right, right from the start.

Expand Your In-house Capabilities

Advanced tools to enhance your cell line development.

Increase Efficiency

High-throughput systems reduce cost and shorten timelines for stable, scalable, productive cell lines.

Generate Clonal Cell Lines Faster

Streamline Cell Line Development with Automated, High-Throughput Instruments

For high yields of recombinant proteins for therapeutic applications, stable, scalable and high-titer cell lines are needed.  With advanced techniques and technologies from Sartorius, you can accelerate cell line selection and development, so you screen more clones faster and make decisions sooner.

The iQue® Advanced Flow Cytometry Platform provides cell count and viability data to quickly select the best pools.

iQue® for Deeper Insights Delivered Faster

The iQue® Advanced Flow Cytometry Platform with iQue® Human IgG Titer and Viability Kit provides greater insight into production clone ranking and selection, with rapid and simultaneous evaluation of IgG titer, specific productivity, and cell health.  

  • Microfluidics acquisition capability analyses samples as small as 10 μL in a 384-well format with zero dead volume. Cell detection at rates of thousands of cells per second 
  • Multiple readouts for every event 
  • Natural conformation of cells and targets

Learn More About iQue®

The Octet® BLI is an easy-to use, real-time analytical tool that rapidly screens critical quality attributes.

Octet® Label-Free Biomolecular Interaction Analysis 

Octet®️ Label-free Bio Layer Interferometry (BLI) technology uses optical biosensors to measure protein-protein interactions in parallel, without the use of detection agents. This robust and fluidics-free approach enables fast characterization of expressed proteins even in complex and unpurified samples in real-time. 

  • Rapidly determine product titer and binding kinetics 
  • Monitor native states label-free and in real-time 
  • Use both purified and unpurified samples 
  • Recover sample plates after non-destructive measurement

Explore Octet® BLI Label-Free Detection System

Ambr® 15 Cell Culture Generation 2: Automated Bioreactor System 

The leading microbioreactor system for cell line development and process optimization, allowing parallel operation of up to 48 single-use microbioreactors (10-15 mL), controlled by an automated workstation. 

  • Proven industry standard for high-throughput cell line development  
  • Enables rapid evaluation of multiple bioreactor cultures, increasing productivity and saving on materials and labor 
  • Provides predictive performance to larger-scale bioreactors 

Explore Ambr® 15


Automated cryovial filling system for cell banking and strain banking applications

Fill-It: Automated Cryovial Processing for Cell Banking Applications

The Fill-It system helps create cell banks for drug discovery, screening operations and GMP production. It rapidly dispenses cell suspensions, strains or reference standards from bulk stock into the screw-cap cryovials before recapping them.  

  • Fits in a standard biosafety cabinet  
  • Facilitates easy loading and operation 
  • De-caps racks of cryovials held in either 96-, 48- or 24-vial racks. 
  • Provides automated cell banking compatible with wide microtube range

Learn More About Fill-It


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Cell Line Development Webinar Series

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Featured Resources

Accelerating CLD with a Combined Platform Approach

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Optimize Your Cell Line Development Workflow

Accelerate Process Optimization by Combining Cell Culture with Titer Measurements.

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Webinar: Effective Cell Line Development

Reducing Risk, Decreasing Timelines, and Optimizing Outcomes

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Accelerating Process Optimization by Combining Cell Culture With Titer...

Cell line development (CLD) involves the screening of thousands of clones to find those that are stable, produce high yields of the bioproduct, and ha...

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Octet® R Series Glycan shadow visual

Mannose Glycans Content Screening for Human-mAb Samples

A novel approach for relative mannose content screening and ranking of human monoclonal antibody (mAb) samples using the Octet® GlyM Kit

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Application Note: iQue® Mouse IgG Type and Titer Assay Kit

A Novel Solution to Expedite Antibody Discovery

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Cell Line Development: Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform

Application Guide: Cell Line Development

Accelerating Antibody Discovery by Monitoring Titer and Glycosylation With the Octet® Platform

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Related Resources

Brochure

Ambr® 15 Cell Culture Generation 2

PDF | 3.8 MB
Brochure

Advanced, high-throughput flow cytometry solution to speed up your entire workflow 

PDF | 3.2 MB
Brochure

Automated cell banking system to create cell banks of higher quality, consistency and viability 

PDF | 1.4 MB
Brochure

Generation 2 automated bioreactor system to accelerate cell line development 

PDF | 5.2 MB
Brochure

Insightful Solutions for Biologics Drug Discovery with a focus on Monoclonal Antibodies (mAbs)

PDF | 3.8 MB
Application Note

Quantitatively Screen Bispecific Antibodies Using Protein A and Octet® His1K Biosensors

PDF | 737.4 KB
Application Note

A single, multiplexed assay that simultaneously quantifies IgG isotype, IgG quantity per isotype, total IgG secretion, cell count and cell health

PDF | 1.5 MB
Octet GlyM Kit datasheet
Datasheet

GlyM Kit Datasheet

PDF | 781.3 KB
Datasheet

Facilitated production of a cell bank using an automated cryovial dispenser 

PDF | 3.5 MB
Octet GlyM Kit Technical Note
Technical Note

GlyM Kit Technical Note

PDF | 2.2 MB

Frequently Asked Questions

No, Octet cannot separate charge variants of antibody from glycosylation. Octet Glycan screening kits (GlyM and GlyS) are lectin based and recognize specific sugar moieties, but not charge variation.

Yes, you can. This is a typical application in a process screening application and determine most sensitive factors and find suitable ranges. Often, this could be followed up on with more detailed studies using an Ambr® 250 for easier scale-up.

During ambr15 runs, daily cell sampling is necessary to track both cell count and viability. The iQue Cell Count and Viability kit is perfectly designed for the reproducible analysis of cell count and viability to screen and profile cells. The small sample size (10 uL) necessary allows for the preservation of precious sample and this coupled with the streamlined workflow allows for rapid determination of the health of each culture. 

The iQue Human IgG Titer and Viability kit  together with the iQue platform can be used to calculate titer. One of     the key benefits of this kit is that it provides such metrics as IgG quantitation per viable cell. The iQue Forecyt software is capable of automatically ranking this data to allow the user to make informed decisions on     clone selection.  The detection range of 0.6 ug/mL to 20000 ug/mL makes it more suitable for early-stage cell line development including clone selection. There is also a mouse version of this kit available too. 

Yes, you can. For example, you can also use Protein L-based sensors suitable for certain antibody fragments. In addition, you can quantify certain glycan classes like mannosylated and sialylated species.

Fortunately, for this assay you are mostly using diluted samples (about 10-20x), which reduces the impact of any matrix effects. Still, if you would like to assess any potential impacts, we recommend performing standard addition studies to quantify.

Critical quality attributes (CQAs) of cell lines include identity, microbiological sterility, genetic fidelity and stability, and viability. In terms of characterizing the biopharmaceutical product the CQAs are extended to potency, and biological activity. The later includes the glycosylation pattern, which has implications on the half-life, immunogenicity, and pharmacokinetics of the biologic, and must be closely monitored throughout drug development and manufacturing.

Are you tired of seeding thousands of cells in large stacks of cell culture plates only to find out they are either no single cells, viable or productive? Tired of screening hundreds of plates for the one clone that has it all and will fulfill your projects titer goal? Why leave your pharmaceutical cell line development to chance? With the CellCelector™ single cell and colony picking platform you can now effectively assess and verify your clones before deciding which ones to choose. Within under a week you will get from a pool of single cells to plates full of clones that have proven to be monoclonal, viable and productive. Instead of relying on large quantities of plates to produce a winner you can now use actual data reliably predicting the future of your clones. This saves consumable and media costs, incubator space but most of all valuable time to reach your project goals by avoiding missteps, a second cloning round and unnecessary procedures.

Automated monoclonality proof: robust single cell detection in nanowells

In a conventional single cell cloning workflow single cells are seeded in 96 well plates making reliable automated single cell detection in bright field difficult as cells are often settled at the very edge of the well. Thus, the monoclonality status of a given clone at day 0 is usually checked manually or retroactively once the clone has grown. But why search a single cell within a well area that is more than 100 times larger than the surface occupied by a cell? With the CellCelector™ HT-NIC approach the cells are separated and clearly visible within 200 µm large nanowells and can therefore be identified reliably and automatically by software. Even just after seeding or when they touch the nanowell border.

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