iQue® Advanced Flow Cytometry for Cell Line Development
Rapid Clone Evaluation and Better Clone Selection
The iQue® Advanced Flow Cytometry Platform with iQue® Human IgG Titer and Viability Kit is a high-throughput suspension cell and bead-based analysis platform for clone screening. It provides greater insight into production clone ranking and selection with rapid and simultaneous evaluation of IgG titer, specific productivity and cell health.
The iQue® Platform for Cell Line Development features:
- Microfluidics acquisition capability analyzes samples as small as 10 μL in a 384-well format with zero dead volume
- Screen your clones in days, not months with cell detection rates of thousands of cells per second
- Multiple readouts for every event
- Screens cells in suspension and targets in their natural conformation
iQue® Advanced Flow Cytometry Reagents
iQue® Platform for Cell Line Development Allows You To:
Enjoy a simple, scalable, multi-user environment with walkaway automation, comprehensive analysis and visualization tools and other features not found in traditional solutions.
Relieve clone selection bottlenecks with rapid and simultaneous evaluation of IgG titer, specific productivity and cell health.
Significantly speed up decision making with novel visualizations that facilitate the fastest path to actionable results, even from complex data sets.
Frequently Asked Questions
The iQue Human IgG Titer and Viability kit - together with the iQue® platform - can be used to calculate titer. One of the key benefits of this kit is that it provides such metrics as IgG quantitation per viable cell. iQue® Forecyt software is capable of automatically ranking this data to allow users to make informed decisions on clone selection. The detection range of 0.6 ug/mL to 20000 ug/mL makes it suitable for early-stage cell line development, including clone selection. There is also a mouse version of this kit available.
During ambr®15 runs, daily cell sampling is necessary to track cell count and viability. The iQue® Cell Count and Viability kit is perfectly designed for reproducible analysis of cell count and viability to screen and profile cells. The small necessary sample size (10 uL) allows for preservation of precious sample. This, coupled with the streamlined workflow, allows for rapid determination of the health of each culture.