Octet® Label-free Biomolecular Analysis for Cell Line Development
Measure Biomolecular Interactions in Real Time
The Octet® BLI Label-Free Detection System eliminates issues with detailed characterization of expressed proteins, thanks to optical biosensors that measure molecular interactions in real time (without detection agents) and advanced, fluidics-free direct detection of specific biomolecules. This high-throughput system rapidly determines product titer, screens for specific glycosylation content and performs off-rate analysis to help you select optimal clones using purified and unpurified samples.
The Octet® BLI Label-Free Detection System for CLD offers:
- High-throughput — Parallel processing of up to 96 samples
- Accelerated time to results — Protein quantitation in as little as two minutes
- Quick identification — Rapidly determine product titer and binding kinetics
- Save time — Monitor native states label-free and in real time
- Crude sample compatibility — Use purified and unpurified samples
- Economical — Recover sample plates after non-destructive measurement
Octet® System Features for Cell Line Development
Replace enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC) with Octet® Label-Free Detection Systems for quantitation of antibodies and recombinant therapeutic proteins. Accurate and fast assays in real time are easy to develop and transfer to quality control (QC) and process development.
Accelerate antibody discovery by combining titer data with sialic acid or mannose statistics for more informed decisions. Our glycosylation kits can screen and quantify proteins in crude and purified samples and reduce preparation time by up to three hours.
The Octet® BLI platform is an easy-to use, real-time biologics analytical tool that requires no labeling and enables rapid screening of critical quality attributes earlier in the cell line development workflow.
Frequently Asked Questions
What possible interferences from cell broth should I be aware of when measuring titer using the Octet® System?
Fortunately, for this assay you are mostly using diluted samples (about 10-20x), which reduces the impact of any matrix effects. Still, if you would like to assess potential impacts, we recommend performing standard addition studies to quantify.
Yes, you can. For example, you can also use Protein L-based sensors suitable for certain antibody fragments. Additionally, you can quantify certain glycan classes like mannosylated and sialylated species.