High Performance, Label-free Kinetics Characterization 

Octet® R2 Protein Analysis System: Grows With Your Biomolecule Characterization Needs

The Octet® R2 System makes high-quality interaction analysis affordable and flexible. Priced for startup academic and biotech labs and low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The 2-channel Octet® R2 system offers an advanced, fluidics-free approach to interaction analysis, with a wide variety of off-the-shelf Dip and Read biosensors for binding kinetics and quantitation analysis. Octet® R2 provides a low-cost entry point for labs with lower throughput needs, while providing the flexibility to field-upgrade to 4 or 8 channels anytime the need arises.

The Octet® R2 System characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multipurpose system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates. With sensitivity for small molecule analysis, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates.

  • Budget-Friendly - Designed for academic and startup labs. Low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system
  • Flexible second channel for use as a reference or more samples - Unique 2-channel design has dedicated spectrometers for simultaneous referencing or added capacity.
  • Grows With You – Decide later to upgrade to a 4-channel or 8-channel Octet® R- Series system for higher throughput. Upgrades are performed within a single visit by a field service engineer, without the hassle of trading in the old model 

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Key Features of the Octet R2 BLI System

The Benefits of the Octet® R2 Protein Analysis System

Octet R2 label-free kinetics characterization instrument
Kinetic Analysis

The Octet® R2 System monitors binding events in real- time measuring binding association  (ka) and dissociation (kd) kinetics, and affinity constants (KD). The system’s two channels can be used independently for screening purposes, or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization. 

Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. Pre-defined templates in Octet® BLI Discovery software streamline setup prior to running an assay and minimizes training needs. The software also offers users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.

Quantitative Measurements

The 2-channel Octet® R2 system directly detects the presence of specific proteins in solution with minimal interference from complex matrices. Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample using a simple, one-step assay.

Sample Plate Cooling for Temperature-Sensitive Proteins

The sample plate temperature can be controlled from 15–40°C, which enables reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic parameters.

Biosensor Re-racking

The Octet® R2 system provides the ability to re-rack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.

Easy to Use

Robust technology with simple, integrated user interface designed for biologists. Spend less time learning instrumentation and more time deriving insights.

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Applications for the Octet® R2

Large Molecule Characterization: Binding Rate Graph

Large Molecule Characterization

With pre-defined templates to follow in the Octet® BLI Discovery software, the Octet® R2 system streamlines setup prior to running an assay and minimizes training needs. Octet® Analysis Studio then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface.

Small Molecule Characterization chart

Small Molecule Characterization

With sensitivity to screen small molecule fragment libraries, the Octet® R2 instrument enables the measurement of interactions between small molecules down to 150 Da in size to determine accurate kinetics rates.

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Featured Octet® BLI Resources

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Educational Resources

7000+ Octet publications New

Octet® Publication Database

Literature references covering a large variety of research areas and applications

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Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

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White Paper

Writing Grant Proposals for Biomolecular Interactions Research

Tips collected over the years from customers successful with their equipment grant proposals.

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

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Technical Note

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

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Application Guide

A Simple Method to Determine Relative Potency (EC50)

Using Octet® BLI Technology

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Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Case Study

Bispecific Antibodies: From Research to Clinical Application

Learn how Octet BLI transforms our approach to drug discovery and development

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Product Resources

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Octet® R2 System Datasheet

PDF | 485.0 KB
Octet®️ Systems Platform Brochure

Octet®️ Systems Platform Brochure

PDF | 1.3 MB
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Octet®️ BLI Discovery and Octet®️ Analysis Studio Software

PDF | 1.7 MB
Datasheet: Octet Data Analysis HT Software Version 13

Datasheet: Octet® Software Version 13

PDF | 409.1 KB

Octet System Qualification Products and Services

PDF | 99.8 KB

New Octet®️ R Series of Systems Flyer

PDF | 523.8 KB
selection guide

Octet® BLI Biosensor Selection Guide

PDF | 404.8 KB
Detection Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Biosensor Type

Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray

Data Presentation

  • Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits 
  • Concentration data analysis including calibration curves and output of tabulated concentration data
  • Epitope binning and cross-blocking matrices and trace overlays
  • Tabulated kinetic data

Sample Type

Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and virus-like particles in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants and crude cell lysates

Number of Spectrometers


Maximum Simultaneous Reads


Data Collection Rate

2, 5 or 10 Hz

Sample Position and Format

1 standard 96-well, black, flat-bottom microplate

Sample Volume

180–220 µL/well
Nondestructive testing

Orbital Flow Capacity

Static or 400–1500 rpm

Analysis Temperature Range

15°C to 40°C, in 1°C increments

Quantitation and Kinetics


Up to 2 assays in parallel

Molecular Weight Detection

>150 Da

Analysis Time per Sample

hIgG quantitation in 2 minutes for 2 samples
Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
Association Rate Constant (ka)101 to 107 M-1 s-1
Dissociation Rate Constant (kd)

10-6 to 10-1 s-1

Affinity Constant (KD)

1 mM – 10 pM

Quantitation Range

0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor) 
Protein - and assay-dependent

Baseline Noise (RMS)

<3.0 pm

Baseline Drift

< 0.1 nm/hour

Quantitation Precision

CV <10%



72 lb (32.7 Kg)

Dimensions (H x W x D)

19.5 in x 22 in x 18.2 in
(49 cm x 56 cm x 46 cm)

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