Drop. Read. Done.
Is UV-Vis spectrophotometry enough for your research? Discover label-free, real-time quantification and characterization of proteins in a 4 µl miniature drop.
Simple. Rapid. Accurate.
The Octet® N1 System provides label-free protein analysis in microvolumes at an affordable price, making it accessible to all. This personal assay system enables protein quantitation and kinetic experiments with sample microvolumes of just 4 µL—right at your lab bench. With specific biosensors, it delivers precise target protein quantification, even in the presence of other proteins and biomolecules.
- Simple and rapid – The Octet® N1 System enables the detection of specific monoclonal antibodies (mAbs), antibody drug conjugates (ADC), biosimilars and other proteins, even in unpurified samples, in as little as 30 seconds.
- Save sample – Measure simple concentration analysis of active analytes or fully characterize binding kinetics and affinity right at the bench using a nanodrop of 4 µL sample.
- Specificity and sensitivity – Take advantage of low concentration measurements for specific target proteins, especially useful in complex mixtures.
Practical Applications:
- Biopharmaceutical Development: Quantification of therapeutic proteins, antibodies, and other biologics during development and production.
- Quality Control: Ensures the accurate concentration of target proteins in manufacturing processes.
- Research and Development: Studying specific protein levels in various experimental conditions, such as in cell lysates, serum, or other biological samples.
Benefits of the Octet® N1 System
Detect the presence or absence of active proteins in a 4 µL drop of sample and easily identify specific proteins in complex solutions. The Octet® N1 System surpasses existing methods like UV-Vis spectrophotometry, ELISAS, blots and gels, and its real-time binding curves provides instant visual confirmation.
Measure protein concentration without labeling or secondary detection reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the Octet® N1 System provides accurate quantitation in seconds, enabling informed decisions directly within the biomanufacturing process.
Identifying and quantifying specific proteins with HPLC or immunoassay-based techniques like ELISA adds many hours to the process and delays results. Proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples. Additionally, its real-time assays generate binding curves for immediate visual confirmation.
Get rate and affinity constants for binding interactions in minutes using a nanodrop of sample. The compact and affordable Octet® N1 System serves as your personal label-free kinetics solution. Ready to use straight out of the box, its simple workflows allow you to run assays easily at your lab bench.
The Octet® N1 System enables real-time monitoring of binding interactions under changing conditions, allowing you to develop better immunoassays in just minutes. Find the best antibody pairs, identify the best assay parameters, and even build and optimize an ELISA step by step.
Applications
Easily Determine Interactions Kinetics
Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4 µL of sample. With both a small price and a small size, the Octet® N1 system can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.
Instantly Detect Presence of Specific Protein
Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the Octet® N1 system complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.
Rapidly Quantify your Protein with Confidence
Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the Octet® N1 system gives you accurate quantitation in seconds compared to traditional methods that can often take hours.
Conclusion
Nanophotometers measure total protein concentration and purity ratios but cannot differentiate between proteins in a sample. In contrast, the Octet® N1 excels in applications requiring specific quantification of target proteins in complex mixtures, offering high sensitivity and specificity. This makes it ideal for biopharmaceutical development, quality control, and research applications where precise protein measurement is critical.
Featured Resources
Power | 100–240 V AC, 0.2–0.1 A, 50/60 Hz, 8 W (18 W peak) |
Technology | Bio-Layer Interferometry (BLI) |
Sample Volume | |
| Weight | 7.2 lb (3.3 kg) |
| Dimensions | 6.8” H x 6.0” W x 8.7” D (17.4 cm H x 15.3 cm W x 22.2 cm D) |
