High Throughput System for Biologics Discovery, Development, QC, and Manufacturing

The Octet® R8 label-free protein analysis system provides fast, high throughput, and accurate characterization of biomolecules and is compatible with longer experimental run times and seamless integration into GxP-compliant labs during biologics development. 

The 8-channel Octet® R8 system performs quantitation and kinetic analysis of up to 96 samples in 30 minutes to 2.5 hours, depending on the specific assay. An optional microplate evaporation cover minimizes losses in sample volume, allowing post-analysis sample recovery even after an experiment.

The Octet® R8 system can be used for a wide range of analyses including kinetic analysis, titer of IgG and other proteins, reagent qualification, immunoassay development, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, small molecule analysis, elucidating cell signaling mechanisms, and infectious disease monitoring. Analysis can be done using a single channel or up to eight channels, enabling flexibility in sample throughput.


  • Unmatched Flexibility and Versatility -  Eight parallel, independent channels provide maximum speed, sensitivity, and flexibility
  • Unattended Run Time – An optional evaporation cover facilitates up to 12 hours of unattended run time, while minimizing losses in sample volume and allowing post-analysis sample recovery even after an experiment
  • Upgrade to GxP -  The Octet® R8 GxP package is available with 21 CFR Part 11 software and IQOQPQ kits, protocols, and services for regulatory compliance. Choose when to upgrade a non-GxP system to a GxP system for regulatory compliance.

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The Octet® R8 Protein Analysis System Features

Octet R8 high throughput label free detection instrument
High-Quality Kinetic Screening and Affinity Characterization

The Octet® R8 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and affinity constants (KD).

Versatility to Detect a Wide Range of Molecules

The high sensitivity Octet® R8 system detects a diverse range of biomolecules from small molecules to proteins to cells.

Eight Parallel, Independent Channels for Maximum Speed and Flexibility

The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.

Perfectly Suited to Operate in GxP-Regulated Environments

Optional 21 CFR Part 11 software for Octet® R8 system available along with installation and qualification (IQOQ) and performance qualification (PQ) kits.

Sample Plate Cooling for Temperature-Sensitive Proteins

The sample plate temperature can be controlled from 15–40°C, enabling reliable kinetic determinations at different temperatures for temperature-sensitive proteins. Additional advantages of sample cooling include the ability to rapidly determine binding rate constants at multiple temperatures to extrapolate thermodynamic measurements. The Octet® R8 system’s eight channels can be used independently to measure samples for screening purposes or in tandem, pairing the sample read with a dedicated reference for high-quality kinetic characterization.

Fluidics-Free Dip and Read Format Reduces Assay Time and Maintenance Cost

The Octet® platform provides faster time to results and process economy when compared to other technologies due to its fluidics-free design. Additionally, crude sample compatibility saves valuable time by eliminating the need for sample pre-treatment, and non-destructive testing conserves precious samples for other assays.

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Applications

Kinetic and Affinity Characterization chart

Kinetic and Affinity Characterization

The superior sensitivity of the Octet® R8 system enables measurement of both small and large molecules and kinetic constants over a broad range. The system accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics.

With ready to use biosensors for most proteins, the unique Dip and Read approach streamlines workflows and expedites assay development. It allows for direct measurement of crude samples with minimal instrument maintenance.

Protein Quantitation in Bioprocessing chart

Protein Quantitation in Bioprocessing

Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample or 32 minutes for a whole 96-well plate using a simple, one-step assay.

High sensitivity in quantitation can be easily achieved by increasing read time and shake speed, and even higher sensitivity can be achieved to sub-ng/mL levels with 2-step or 3-step assay formats, allowing automated measurement of contaminants such as host cell proteins and residual protein A, faster and more precisely than ELISA. Process economics can be improved further by regenerating and reusing the biosensors.

Quality Control (QC) and Manufacturing chart

Quality Control (QC) and Manufacturing

The Octet® R8 system has been developed to operate reliably in regulated quality control (QC) environments. The Octet® is aptly used in method development and validation for lot release and in-process testing for ligand binding, concentration, and impurity analysis in both upstream and downstream processes.

The ease of use and simplicity in design together with 21 CFR Part 11 software and a full line of GxP products and services makes the Octet® R8 system an ideal analytical instrument for compliant labs.

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Literature references covering a large variety of research areas and applications

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Application Note

Biomolecular Binding Kinetics Assays on the Octet® Platform

Introducing label-free platforms, the technology, capabilities, and advantages of label-free binding assessment

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Application Guide

Ligand Binding Assays That Meet Compliance with Octet® BLI Systems

Octet® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...

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White Paper

Writing Grant Proposals for Biomolecular Interactions Research

Tips collected over the years from customers successful with their equipment grant proposals.

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Brochure

Octet® R Series Systems

Accelerate your research with an upgradeable, label-free protein analysis platform

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Webinar

Comparability Studies: Minimize Time, Maintain Quality

Developing Methods for Comparability Studies of Therapeutic Monoclonal Antibodies

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

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Technical Note

Octet® Kinetics Assay Method Development Guideline

Developing kinetics binding assays to establish specificity, accuracy, precision, linearity and range as stipulated by USP 1032

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Application Guide

A Simple Method to Determine Relative Potency (EC50)

Using Octet® BLI Technology

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Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Product Resources

Datasheet

Octet® R8 System - Unmatched Flexibility and Versatility for Biologics Discovery, Development and Quality Control

PDF | 905.0 KB
Octet®️ Systems Platform Brochure
Brochure

Octet®️ Systems Platform Brochure

PDF | 1.3 MB
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Datasheet

Octet®️ BLI Discovery and Octet®️ Analysis Studio Software

PDF | 1.7 MB
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Datasheet

Octet® CFR Software V12.X and Software Validation Package Datasheet

PDF | 197.7 KB
Datasheet: Octet Data Analysis HT Software Version 13
Datasheet

Datasheet: Octet® Software Version 13

PDF | 409.1 KB
Datasheet

Octet System Qualification Products and Services

PDF | 99.8 KB
Octet® AS Offline Biosensor Immobilization Station Datasheet
Datasheet

Octet® AS Offline Biosensor Immobilization Station

PDF | 183.1 KB
Flyer

New Octet®️ R Series of Systems Flyer

PDF | 523.8 KB
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Flyer

Octet® BLI Biosensor Selection Guide

PDF | 404.8 KB
Octet Instrument Comparability Kinetics Precision Assessment
White Paper

Instrument Comparability Assessment: Kinetics Precision Assessment in Ligand Binding Assays on the GxP-Compliant Octet® RED96e and Octet® R8 Instruments

PDF | 1.3 MB
Detection Technology

Bio-Layer Interferometry (BLI)

Biosensor TypeDisposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the biosensor tray

Data Presentation

Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits
Concentration data analysis including calibration curves and output of tabulated concentration data
Tabulated kinetic data
Epitope binning and cross-blocking matrices and trace overlays

Sample Type

Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and VLPs in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates

Number of Spectrometers

8

Maximum Simultaneous Reads

8

Data Collection Rate

2, 5 or 10 Hz

Sample Position and Format

1 standard 96-well, black, flat-bottom microplate

Sample Volume

180–220 µL/well
Nondestructive testing

Orbital Flow Capacity

Static or 100–1500 rpm

Analysis Temperature Range

15°C to 40°C, in 1°C increments

Quantitation and Kinetics

Throughput

Up to 8 assays in parallel

Molecular Weight Detection

>150 Da

Analysis Time per Sample

Real-time kinetic binding experiments from 5 minutes to 4 hours, or up to 12 hours at 25°C with the evaporation cover
Association Rate Constant (ka)101 to 107 M-1 s-1
Dissociation Rate Constant (kd)

10-6 to 10-1 s-1

Affinity Constant (KD)

1 mM – 10 pM

Baseline Noise (RMS)

<3.0 pm

Baseline Drift

< 0.12 nm/hour

Quantitation

Workflow

Up to 8 assays in parallel

Analysis Time per Sample

Human IgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples

DirectQquantitation Range for Human IgG with Protein A Biosensor

0.05 –2000 μg/mL

Instrument

Weight

72 lb (32.7 Kg)

Dimensions (H x W x D)

19.5 in x 22 in x 18.2 in
(49 cm x 56 cm x 46 cm)


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