Application Note: Analysis of Fc-gamma Receptor-IgG Interactions on the Octet® Platform
Authors: Renee Tobias, Danfeng Yao, and Sriram Kumaraswamy | Sartorius, Fremont, California
Overview
A major mechanism of action for therapeutic monoclonal antibodies is the initiation of effector function via binding to Fc gamma receptors (FcγRs) expressed on the surface of immune effector cells. The ability of therapeutic monoclonal antibodies to bind FcγRs can greatly impact their safety and efficacy, and as such, efforts to analyze and enhance Fc interactions with FcγRs are an integral part of biotherapeutic development processes.
The Octet® BLI platform offers a high-throughput, rapid, flexible, and sensitive approach for the accurate and reliable measurement of the binding affinities of Fc gamma receptors to monoclonal antibodies. Octet® BLI systems utilizes Bio-Layer Interferometry (BLI), a label-free technology that measures molecular interactions in real-time for the purpose of detection, quantitation, and kinetic analysis.
- Document type: Application Note
- Page count: 18
- Read time: 40 minutes
Key Takeaways:
- Rational supporting the Octet® BLI platform as the technology of choice for analyzing Fc gamma receptor-IgG binding interactions
- Advantages for detailed characterization of Fc receptor/IgG interactions in terms of throughput, ease of use, specificity, and flexibility
- Best practices regarding biosensor selection, ligand loading, and analyte concentration/association
- Key considerations for assay optimization, data acquisition, curve fitting and analysis of results
- Yielding accurate, reproducible data and reliable kinetic and affinity constants
Workflows Supported
Measuring accurate and reliable kinetics of interactions between Fc gamma receptors and monoclonal antibodies can be challenging, but is a critical application in many stages of biopharmaceutical development including:
- Antibody Engineering
- Comparability Studies
- Lot Release Assays
- Quality Control
This resource is designed for researchers working in the areas of:
- Manufacturing
- Quality Control
