A Simple Method to Determine Relative Potency (EC50) Using Octet® BLI Technology Application Guide

Authors: Stuart Knowling, PhD, Senior Scientist | Last updated: 25/August/2022


Octet® BLI Discovery and Analysis Software now contains a new module for quickly and easily assessing relative potency using the same assay format currently used for quantitation and kinetic assays, allowing you to measure EC50 similarity using a range of parameter logistic equations (PL), linear and semilog lines. This document introduces basic assay design and an introduction to the new software module.

  • Document type: Application Guide
  • Page count: 6
  • Read time: 10 minutes

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Key Takeaways

  • New EC50 and IC50 analysis capabilities to determine drug product activities using surrogate potency assays, reducing assay variability, timelines, and costs
  • Data bridge to support data transfer between Sartorius Octet® and Sartorius Ambr® bioreactor systems to accelerate cell line development workflows

This Resource is Designed for:

  • Biopharma
  • CLD
  • Academic
  • Biotech
  • Pharma QC

Applications Supported:

  • Biologics
  • Lot Release
  • Label-free kinetic
  • Affinity
  • Epitope binning
  • Activity
  • Concentration
  • Potency
  • Screening

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