High Throughput Platform for Biologics Development

From IgG titer to epitope binning, the Octet® RH16 system offers label-free biomolecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.

High detection sensitivity, throughput, and 384-microwell plate compatibility of the Octet® RH16 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding campaigns. The Octet® RH16 instrument also supports additional automation for extending walk-away times and reducing operator input.  Target ligands can be batch immobilized onto biosensors and returned to the biosensor tray for re-use in future experiments, thus extending the number of uses for each biosensor, preserving valuable ligand, and maximizing flexibility in experimental design.
 

  • Analyze up to 16 samples simultaneously
  • Streamline workflows with automation - Automation ready and robot compatible for microplate and biosensor tray loading.
  • GxP packages available - Available as complete packages with 21 CFR Part 11 software and IQOQ/PQ kits, protocols and services for regulatory compliance.

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Boost your research and commercialization goals by combining BLI and SPR at an attractive promotional price. 

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Discover the Octet® RH16 & RH96 Systems

Features of the Octet® RH16

Supports 96-well and 384-well microplate formats

Arrange assay samples in 96- or 384-well plates depending on workflows and sample volumes

Two microplate locations to maximize throughput and improve workflow

Supports larger sample and reagent capacities increasing walk-away times

Microfluidics-free Dip and Read format reduces assay time and maintenance cost

The Octet® RH16 system utilizes Dip and Read technology where biosensors dip into samples housed in microplates. With no fluidics involved, Octet® systems have more uptime and lower operational costs

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Applications

ELISA Conversion

ELISAs are known for having variability in results, susceptibility to human error, and slow times-to-results. High throughput Octet® systems are designed to overcome those limitations.  Convert your ELISA assay into an Octet® assay.

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Epitope Binning

The epitope binning process identifies and arranges antibody candidates that bind similar epitope regions to an antigen into bins. Octet® epitope binning assays rely on testing the sequential binding ability of two antibodies to an antigen. Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet® platforms ideal for analyzing large antibody libraries. 

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protein contaminant diagram

Protein Contaminant Testing

Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits  provide biologics developers with a fast and easy-to-use assay for contaminant testing.

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Featured Publications

Webinar

Integrating Octet® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

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Application Guide

Ligand Binding Assays That Meet Compliance with Octet® BLI Systems

Octet® GxP packages support the GMP compliant implementation, including 21 CFR Part 11 software, validation packages, and biosensor validation support...

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Application Guide

Accurate Measurements on the Octet® Platform

Without Added Sample Purification Steps

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Label-Free Detection technologies, key considerations and applications cover
eBook

Label-Free Detection Technologies: Key Considerations and Applications

Technologies, Key Considerations, and Applications

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Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered

Data presentation

Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts

Automation

Robot compatible microplate and biosensor tray loading

Orbital flow capacity

Static or 100–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (ka)

101 to 107 M-1 s-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM to 10 pM

Baseline noise (RMS)

≤ 4 pm (RMS)

Baseline drift

< 0.1 nm/hour

Quantitation range

8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm

Quantitation precision

CV <10%

Weight

150 lb (68.2 Kg)

Dimensions (H x W x D)

30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well and 384-well, black, tilted bottom microplates

Molecular weight detection

>150 Da (8–16 biosensors)


Octet® RH16 System Datasheet
Datasheet

Octet® RH16 System Datasheet

PDF | 1.6 MB
Octet®️ Systems Platform Brochure
Brochure

Octet®️ Systems Platform Brochure

PDF | 1.3 MB
Octet® 384-Well Tilted-Bottom Microplate Datasheet
Datasheet

Octet® 384-Well Tilted-Bottom Microplate Datasheet

PDF | 352.5 KB
datasheet thumbnail
Datasheet

Octet® CFR Software V12.X and Software Validation Package Datasheet

PDF | 197.7 KB
Datasheet: Octet Data Analysis HT Software Version 13
Datasheet

Datasheet: Octet® Software Version 13

PDF | 409.1 KB
datasheet thumbnail
Datasheet

Octet®️ BLI Discovery and Octet®️ Analysis Studio Software

PDF | 1.7 MB
Datasheet

Octet System Qualification Products and Services

PDF | 99.8 KB
Octet® AS Offline Biosensor Immobilization Station Datasheet
Datasheet

Octet® AS Offline Biosensor Immobilization Station

PDF | 183.1 KB
selection guide
Flyer

Octet® BLI Biosensor Selection Guide

PDF | 404.8 KB
app note cover
Application Note

Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics

PDF | 372.5 KB
Octet RH16 with Lab Tech
Application Note

Converting ELISA Assay Into Octet® Quantitation Assay

PDF | 207.5 KB

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